Comment: 0-22 hr AEL
CAGTGTGCATGTGTTTGTTTTTTTTCCGTTTGCAACTCAAGTAACTTCAAACTAATAATGGCTTAAACAATAATTGGCTT ATTTTCAGTACAAATACAAAGCACAAAAAAACAAAAAATCACAAAAAAATAAAACGAAACGAGAGCATATAACATAAAAA CCCAAAACAAAAACGATCGGCTCAGCAGAGCCAACGAAAAGCAAGCAGCGTAGCAGCAGGAGCAGAGGATGCACCACTGA TCCCGGAGAGCTCGAGACATGAGAAGCGAAACACCGAGCAGCCACCAAGCATCGACTTATGTAGCGGTCCATGACGAGCA TTAAGACCGAGATGCCGCCCCTGCACGCGGCAGAGGCGCTCGCCTCCAGCAGTGCTACAGACAGTGGTGGGGGAGGAGCA GGAGGAGGAGGAGGAGGAGGTGGAGGAGGATCGGGCGGACCAGGAGCGGGAGGAACGGGAGGCGTAGGCAGTGCGCCCGC CACACCCAATGCCACAATTAGCGCCGCCGCCGACTCCAGTGATAATCAACCCGGCACGCCGCAGCCCACGCAACAGCAGC AATCGACGCAGCAACAGTTGCAGCAGCCACAATCGCAACAGCAGCAGCAGCAGGCCATGGGTGGAGGAGATCCCCAGCAG CAGCAACAGCAGCAGCAGGTAACGGGTATCACCCACCAGCCGTACGCCACCCACCACATGTACTCCGCATCTGGCGGACA GCAGCAACAACAGCAGCAGATCTACGGCGGACTCTACGGCGGGGACATGCAGCAGCAGCAGGGTTACGCCAGCAGCTACA TCAACAGCTACGAGCAGTTCTACCAGCAGCAACAGCAGCAGCAGCAGGGCACTGATTACGCCTTTGGCACCGTGGGCGTT GACTATGGCAAGAGCGCCGGTGTTCGCTATCATCCCTACCTGCAGACGCCAACCTCCGGACTGGGAGGCATTCCAACGGC CAGCTCGGCACAGGAGGAGGCGGGCAGTGCGCCCAGCGCGGTCAGCACCACCACAGCGGTGGCCATGAGTCCGCGTGTGG TTAGCAGCAGCAGTCCAACGTCGACGTCCTCGCACCTGCAGCTGGGCAGCTCCAGTGGCCAGACACCAGGCTCACCAGGC GGAGCCGCCGGAAGTGCCGGATGCAAGTTGCAGTGCAAGAAGTGCGGCATCCTCACCACCAACGAGTCGGAGCTACAGGA GCACATCGCCAACGTGCACGGAGAGTCGCCGTACGGCAGCAGCGGTTACGCCAGTTCACCGTACATCAAGGAGGAGATGC CCACACCACAGCCACCCGGCGTTGGATCCGCAACGGCCAATCCCGGCGAATTACTCGACCTAGATTCCCAGAAGATGGTC TATCACCAGCAGCTGCTCCAGCAGCAACAACAGCAACACGACGTGGTCGCTGGACTGCCGCTGGGCGCTCTGCCGGATCC GCTGCACTCGATGCAATGTATGCAGCAGCGCGCACTCCACAGCTGGGAGCAGCAGCCACAGCAGACGGTGGCCTCCGTGG AGGGCCTGCCACCCTACATGCAGCAGGGATTGGGCGTGGGGCTGGGAGTCGGTGTGGACAAGTCGCCCTACTACTCACCA AAGCAGTCACCCTATCATCAGTCAACCGGCGTTGGCGGAGCCACGCTGATCAAGCAGGAGTACGGCGGCCACGGACTGAT CAAGTCCGAGTATCCCGATTCGCAGCATTACGTGGACAAGTCCTTCGATCCCACAGCTGGCGGAGCTGGTGGCGCGGAAC TGTGCGCCAGCGTGGCCACCAGTCCGGCGGAGTTCCCCAGCACCACTACGGGCGGACCAGGGCAGGAGGGAGCGGCGGGT GCGGCGCCAGGAGGTGGATACCGCGGTTTCGAGCCACCCAGCTCGAGCTCCGTGCTGCCGGCCAACAGCCTGACGGCCAA GGCGGCCACCTGGAAGTCGAACGAGGCGCGCCGTCCGAAAACGTACAACTGCACGGCCTGCAACAAGTGGTTCACCAGCT CGGGCCATCTTAAGCGCCACTACAATACGACGCTGCACAAGAATGCGGTTAAGTCGAGCGGCCAGCCGGATCCAGCCACC CTGCCCATCTCGGCGCACCACCATCCCGCCCGCGACCCGGTGACGAAGCCCAGTCGCAGGGGCAATGCCGCAGCCGCTGC GGCCGCCGCTGCAGCTGCCGCCTCGGCCAGCGGTCAGGGTCAGCAGCAACAGCCGCCGATACCGCCGCCGCCGGCCAATG TGCCACCCCCGGAGCCGCCTAGAAGTCCGCCCGATTATGGCGGAGGAGGCGGCTTGGGCGTGGGAGCGATGGGCGGCGCT GCCATGTCCCAGTACTCGGCCTCACCCTCGCCCACACAGCAGCAGCAGCACCACCTCAACCACCATCAGCAGCAGGCCAA CGGCTATGCTAATGGCACCGCCAACGGCTATGGCTACATGCAGCAACAAGTGCAATCCACGACAAACGCTTCACCACAGC ACGCTTCCAACAACAACAGCAACAACCAGCAGCAGCAGCAACAACAGCAGCAGCACCATCAGCAGCAGATGCCGCAGCAC CACAACTCCGTTTTGAACGGTCACCCAAACGGGCTAGCAGGTCCCTCCGCCCCACACAACAACAACACCACCCAAATGCC GTCCTCCCAAATGAGGGGCCTGCTGAACGAAACAACAACCACGCCGCCACCAACAACAACAACCCGAGCACCACAAATCA CAACAACAGCAACAACAACAACAACGGCGGCCACCACGGTAGCTATAAAGAGCGAGCAAATGGAGGACAGCAACCACACT CACACCCACACACACACGCATCCCAATCACAGTCACAGCCAGGACAGGAGCCACAGCAGCAGCAGCAGCAGCTCAATGGC CACGGAGGAGGCGGAGGAGCAGGAGCTGCGGGATCAGGAGCAGGCGGACGATCACCTGCATCAGCATCAACAGGCGTCGC AGCAGTATCTGCTGTCGGCGCGTCATTACCACAGCAGCACGCCCAACACGCTCAGCAGCAGCAACACCAATCCCAGCACG CCCAGCAGCAACTCGCCCCATACTATCTACCGGCAGGAGCAGCAGGGAACGGATTTCTCGCGCACCACCCCGCCGCCGCA GCCGCTGCCGCCTATGGGAATGCTGCCGCCTATGGCAATGGACTACAACATGCTGGCTTTGGATATGCCCATGCCCATGC CCACGCTCATGCACAGCAATATGCTGCAGTGCAGCAGCACCAGCACCACGCCGCTAGCTACTACCATCACCACCAGTATG CCGGACACTATGCAGCCGCCGCAGCAGCAGCTGGTGCACCACTACCAGGCGGTGCTCCACCCGCTCCATCAGCAGCTGGG GGAGCAGCACCAGCGCCAGGAGGCGGATCATCATCAGCAGCAGCGGGAGCTTCACCAACTGGATCAGCAGCAGCAGCAGC AGCAGGCGTTAATCCTGGCGGACAGTTTGCCGCACAGCAGCAGTTCGCCCACCAGCAGCTCCCCACCGCCCACCATGCCC ATGCCGCTCACCACCATCACAGCGCCGCAGCTGCTGCCGCTGCAGCCGCCGCCGCCGCACATCACCAGCACCATGCCCAT GCCGCCCACCATGCACATGCCCATCATGCCGCCGCCGCCGCAATGCTACCAGCAGCTGCAGCCGCTGGACCCCACAATGA GCTATCACACTATTATTGGTAGTGGCCCGGAGGCGCATACCGGAACGGCCGGCGGTGGCTATAGCAATCAGATCACCACC AGCGATGGCCAGATCCTGCAGCTGATGCCAACATCCCTGTTCGCGCCATATGCACCGCTCTCGCCGTACTCGGTGGCCGC CCAGCGATCGCCGCAGGAGGGCGATCTGCCGCCGGTTCACACACTCACGACGGCCCTGCATGCCCATCAGCAGGGCGGGC AGCAGGAGGCGCAGACGCCAACGCTGACTGTGCTCTCCACGCCCTACTCGCCCACAGTGAGCAGCTCGCGGGCGACTCCC GCACTGGAGATGGATATGGCCACGCTGATGCAGCACCATCAGGACTATGAGATGGAGCAGTACCAGATGCAGCACCAGCA GTTGGATCAGATGCAGCAGCACCAGCAGCAACTGGACCACCAGCAGCAGCAACAGATCCTGGCGGATCAGACCCAGACGA TGGCCCAACAGCCGCTGGCCAAGAAGCGGCGTGGCGGCAACGCAACACCATCGACGACGAAGCGACGGCGGAACAGCAGC GTCGGATCGACGTCGCCGCACTCGACCACCCTGCCATCGGGACGGATCAAGTGTCTGGAGTGCGACAAGGAGTTCACCAA GAACTGCTACCTCACGCAGCACAACAAGAGCTTCCACTCCGGTGAGTACCCATTTCGCTGCCAGAAGTGCGGCAAGCGCT TTCAGAGCGAGGATGTCTACACCACGCACCTGGGTCGCCACCGCACCCAGGACAAGCCGCATAAGTGCGAGCTGTGCCCC AAGCAGTTCCATCACAAGACGGATCTGCGCCGACACGTGGAGGCCATCCATACGGGGCTGAAGCAGCACATGTGCGACAT CTGTGAGAAGGGCTTCTGTCGCAAGGATCATCTGCGCAAGCACCTCGAGACGCACAATCGGCCGCGGGTGGTGGGCAAGA AGTCCGCCGCGGCAGCAGCCGCCGCCGCAGCAGCAGCAGCAGTCACTGCCACGGCGGGATTGGGAGGTGCACCAGCCGCC GGTTCCATTAAGGCGGCATTTGCCCGCTCGCTAACGGTGACGGCTGTGAGCAGTGAGCCAGGCGCAGGAGTTGCAATACC CTCAGCTCCTGCCACTGCCACATGTGCTCCTGTATCTGTATCAGCGTCAGCTCTTGTCCAGGCCGTCAATCCCAGTCTGA CTTCCGGCCTCAGTCTGAAGCGGCACATCGACGATGTGGCCGTTGACGATGACAGCCTGTTGGAGGAGGACGAGGATGAG ATGGAGATGGAGATGGAGATGGATATGTTGGAGGAGGAAGATGAGGAGGAGGAGGAGCTGGGCGATCACCACATGATAAT CAAGAGCGAGTACGTGCAGGAGGAGTTTCAGATGATCGAGAAGAGCATAGAGCTCTACTGAATGAGTGGGCAGGCCACTG GGTTCTGGGTTTTGAAATGCTCCTAGGCTTTGAGCTTGTCTGGGGGATCTGGGGAACGGAGGATCAGCGGTTTAGCGGTT TAGCGGATCACGGGATTATCGGCGGGCAACTGAGCGCGGCTTGGGCTGCGGGCGCAGCTGGAGCGGCTCTGCAGTTGAGT GTCCTGTTCGGGTCAGCTTGGGGTTGTTCCGATTCAGTTTTAACGAGTTTCGCCCGTGCTCGTTAAGAAAAACACACTTT GTTGCTTGGCAGGCAATAATTCGCTGTTTTTGCCGTTTCCCTTGTTAAATTTATTTGTTTTTTTTTTTGTATTTTTTTTT TTATTGTATTCTCTGGGGGGGGATAAACACAAAAGTACTTAGAATTGATCGCAAAGCCGCAGCATTTCAATAACTTAGAC AGCAGGCCGGCCACTCCTTGTCTCGTATCATCATATCATCCATCGTATCATTGTAAGATCGCAACGAAATGCCGAACTGC CAATGTGCCACCCACCGCGCCACAATCCCAATTAATCCATTCATTCCTGTAATTTGTAGTGAGTTTGCCATATGTATGTG TGAAACGATTGAAAGACAGCCAGAATTCCTAATTGTTGTAGCAGCAAATTATTTAATTTTATTTGTATGTTAAAATTGTA TTATTATTATTTGTACTATTTGCGCATAACGCTCCTTATTTGTAAGGCCTTATGATTACGTGTAAACCTAAACCATCAAG TATACCTCAGCAAACAGCAGTCCACGAAACGCTTTAAATCAGGGCGCATATGATTATACATATATAGAGATATATAGATA TTTATACATATTCCATTTTACATGTGTAAACACTAGTTTTAGCACATTTTTTTTTGCTGTATTTTAATTTGTAACCTGTT TTAACTATTATTTGATTATTGATTATGTTTTGTAATCGGGAGGAACGAACAACACACGAATGCCATATACACGATATGTA AACTAAAAATCAGCAACTATATGCCAATTGATATATATATATATACATATATATATATATATATATACTATATCTAGTTA CTAGACCAACGCATTGCATCACGCATATAATATATATGTTAGCCTTAAAAACGCCAAATTGTCTGTCATAGCAAAAGGAA GCAAAAGCAAAAGCAAAAGCAAAAGCAAAAGCAAAAGCAGAAGCAGAAGCAAATGCAAATGGAAAAACCAAGCAAGAAAA TGCGAAATCAGGGATATTATTATGTTTCTAAGCCACAAACGCACGCACCTGTTCGCGTATTATTATACCTACATATATAT GTATTTATTACGTTTAGTTGCCCCTAACTTATGTTCCACCAATAATAAAACCAATTGTAATTATTTAACATTTTCATTTG ATACCCATTTCTAGACTCCCAGCATCTTGTTTGTAATTATTATTATTTACCTTATTTGTTAACCAATTCGATGCTTATCC TCCTTGGCACGCCCACAATTTTTTTTCAATCACAAATATAAAACAAAACCAAAAAAAAAAAAAAAAAA
Cloned cDNAs prepared from polyadenylated RNA isolated from 0-22-hour embryos.
Embryos at 0-22 hr AEL were collected from an isogenic y; cn bw sp (iso-1) strain.
RNA was poly(A)+ selected twice. cDNA made using Stratagene ZAP-cDNA synthesis kit; oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. For clones LD21101 and higher, cDNA directionally cloned into EcoRI/XhoI-digested pOT2 plasmid.
