Comment: 0-22 hr AEL
ACTTCTGATGACGTTTTTTCGTATTTTGAAGGGAATGTAAATGTGGAAAAACTGAGAGATTTGCAACATGCAAACATCCA CGACACGCTAAAAACTCACCGAGTGCCGCCACACGACAATCCAACCGAGAGGAGCACTCCGCACAGCTGGCGGAGCACCC AGAAGGCAGCAGGCGAAAGTTGCGTGGCTGTTGTTTCCGGAAGCGGTGGGCAGAGGGAAATCCGAAGCAGTGGTAGTAGG GAAAGAGGCATCGAAACCCACCCAGCGAAGGCGACCTTGGGAGTGGCAGCGGCCCAATATGCACAACAACTCCACAAAAA CCAAGGAAATGTTCATCGTGCGTGCTCTAGAAAAGATCCTTGCCGATAAGGACATACGGCGCTCCCATCACTCGCAGCTG AAGAAGTCCTGCGATTCGGCGCTGGAGCAGATTAAGGCGGAGCTAATCAGTGCCGGCCAGATCGCAGAGGGCAATGAGCT GCCCTGTGCCGCACTCCCGCTGCCCAAGAATGATGCAGCGAGCATCATAAATGCGGAGACCTACTTTCTCCCCTTCGAGC TTGCCTGCAAGAGCCGCTCGCCCAGGATCGTGGTCACCGCACTGGACTGCCTGCAGAAACTCATTGCCTATGGCCATTTG ACAGGATCCATTCAGGACTCGGCCAATCCGGGTCACCTGCTCATCGACCGTATCGTTGTGACCATATATGGCTGCTTCAG TGGTCCCCAGACGGACGAGGCCGTCCAACTGCAGATAATAAAGGCTCTGCTCACGGTGGTCACCTCGCAGCATGTGGAAA TCCATGAATTCACACTGCTGCAAGCTGTGCGCACCTGCTACGACATCTATTTGTCCAGCAAGAACCTGGTCAATCAGACC ACAGCACGCGCTACGCTCACCCAAATGTTGAACGTGATATTTGCCCGCATGGAGAATCAAGTGTACGAGCTACCACCTCC CAATTCCAATCCCACCAACGGCAGCATCCACTCGGAGGATTGCAATGGCTCGGGAGAGGAGTCGCTGCGGGATTCCGACG AAGTAATTGCCTCGGAACTGCTGGCGGAGATCATATCAGCTGCCTACAATGAGGCGATGAAGGATCAGGAATCGGTCGGT GAGCCAGAGCCAACACTTAATGGAAACGACTACTCCTCGCACTCGGATCACGACAGTGTGGAGCTGCACAGCGAAAACGA TGCGGTTGTAACGGCTAAGTTTACGCACATCCTGCAGAAAGATGCTTTTCTCGTGTTCCGGGCACTGTGCAAGCTATCGA TGAAGCCTTTGCCGGATGGACATCCAGATCCGAAATCGCACGAGCTGCGTTCCAAGGTGCTGTCATTGCATCTGCTGCTG CTCATCCTCCAGAATGCCGGGCCCGTCTTCCGCTCCAACGAGATGTTCATCATGGCCATTAAGCAGTACCTGTGCGTGGC CTTGTCAAACAACGGAGTCAGTCTGGTGCCGGAGGTCTTCGAGCTGTCGCTTTCAATCTTCGTTGCCCTACTCTCGAACT TCAAGGTGCATCTTAAGCGGCAGATAGAGGTGTTCTTCAAGGAAATCTTCCTAAACATTCTTGAGGCGAACTCAAGCAGC TTCGAGCACAAATGGATGGTAATCCAAGCGCTGACACGTATTTGTGCTGACGCCCAGTCCGTGGTGGATATCTATGTTAA TTACGATTGCGACTTTTCGGCTGCAAACCTTTTTGAGAGACTGGTCAACGATCTTTCGAAAATTGCCCAGGGTCGTCAGG CTCTCGAACTGGGCGCCAATCCGATGCAAGAGAAATCGATGCGCATTCGCGGCCTGGAGTGTCTTGTCTCCATTCTTAAG TGCATGGTAGAGTGGAGTAAGGACTTGTATGTTAATCCAAACATGCCGGTTCCACCTATGCAAGTCCAATCGCCGACAAG CACTGAGCAGGATCAGGCGGACACAACTATCCAAACGATGCACAGTGGTTCCAGTCATAGTTTGAACTCCAATCAGGAGC AACTACAGGATCTTCCCGAGGCATTGGAGGAGCGCAAGATGCGCAAGGAAGTGATGGAAACAGGCATTGAGTTATTCAAT CGTAAGCCTCAGAAAGGAGTGCAATTCCTGCAGGAGAAGCAGTTGCTGGGTGCCACATGCGGGGACATTGCGCGCTGGCT GCACGAGGACGAACGACTGGACAAGACAGTGATCGGAAACTACATTGGCGAGAATGACGACCACTCCAAGGAAGTGATGT GCGCTTACATCGATGCCTTTGACTTTCGCCAAATGGAGGTGGTGGCCGCCTTGAGATTTCTTCTCGAGGGGTTCCGCCTG CCAGGAGAAGCACAAAAAATCGATCGGCTGATGGAGAAGTTCGCCAGTAGATACTGCGAATGCAATCCGAAGAACCAGCT ATTCCAAAGCGCAGACACCGTCTACGTGCTGGCATTCAGCATCATTATGCTGACCACGGATCTTCATTCGCCGCAGGTCA AGCACAAGATGACCAAGGAGCAGTACATTAAAATGAACCGCGGCATCAGCGACAGCAAGTCCGATTTGCCCGAGGAGTAC TTGTCGTCCATCTACGACGAGATTTCTGAACACGAAATTAAGATGAAGAACAACTCCGGTATGCTTCAACAGGCGAAACC CACTGGAAAGCAGGCCTTCATAACGGAGAAACGCAGAAAGCTGTTGTGGAACATGGAGATGGAGGTCATCTCGCTGACGG CCACCAATCTAATGCAGTCAGTTTCGCACGTCAAGTCACCCTTCACCTCAGCGAAACACTTGGAGCATGTCCGGCCCATG TTCAAAATGGCTTGGACACCATTTCTGGCCGCTTTCTCTGTGGGTCTCCAGGACTGCGACGATCCTGAGATTGCTACACT CTGCTTGGATGGTATACGTTGTGCTATTCGAATCGCATGCATCTTCCACATGTCCCTGGAGCGAGATGCCTATGTACAAG CCCTGGCCAGGTTTACTCTCCTGAATGCTAACTCGCCCATCAACGAAATGAAGGCCAAGAATATCGATACCATCAAGACG CTTATAATGGTAGCCCACACGGATGGCAATTATCTGGGCAGCAGCTGGCTGGATATAGTGAAGTGCATTAGCCAGTTGGA GCTGGCCCAACTGATCGGCACTGGGGTGCGGCCCCAGTTTCTTTCTGGAGCGCAGACAACGCTCAAGGACTCGCTTAATC CCAGCGTGAAAGAACACATCGGCGAGACGAGCAGCCAGAGCGTGGTGGTCGCAGTCGATCGTATTTTCACCGGCTCAATG CGACTGGATGGCGATGCTATCGTGGACTTCGTGAAGGCCCTGTGCCAGGTGTCTGTGGATGAGCTTCAGCAGCAGCAACC GAGGATGTTCTCCTTGCAAAAGATAGTGGAAATTAGTTACTACAACATGGAGCGTATTCGTCTGCAGTGGTCACGCATTT GGCAAGTTTTGGGTGAGCACTTTAACGCGGTCGGATGCAATAGCAACGAGGAGATCTCATTTTTCGCCCTGGACTCACTG CGTCAGTTGTCGATGAAGTTCATGGAGAAGGGCGAGTTCAGTAATTTCCGCTTCCAGAAGGATTTCCTGCGTCCCTTTGA GCATATCATGAAGAAAAACGCATCGCCGGCAATACGAGATATGGTGGTGCGCTGCATTGCCCAGATGGTAAACTCACAGG CGCATAACATCCGTTCCGGCTGGAAGAATATCTTTAGCATTTTCCACCTGGCAGCGGGAGACAACGAAGAGCCAATTGTG GAGCTGGCCTTCCAAACCACGGGCAAAATCATCGGTGATCTGTACAAGCGTCAGTTCGCCATTATGGTGGACTCGTTCCA GGATGCGGTCAAGTGCCTGTCAGAGTTCGCCACCGCCAGATTCCCCGATACCAGCATGGAAGCCATACGTCTGGTCCGTA CCTGCGCGCAGTGCGTCCACGAGGCACCACAACTGTTTGCGGAGCATGCCGGCATGGAGAACGACGCCTCGGTGGCCGAG GAGGATCGAGTCTGGGTGCGCGGCTGGTTTCCGATGCTATTCTCGCTTTCCTGCGTGGTCAATCGCTGCAAATTGGATGT GCGTACTCGCGCCTTAACCGTGCTTTTTGAGATTGTGAAGACGTATGGTGAGAGCTTCAAGCCCCATTGGTGGAAGGATC TCTTCAATGTGATCTTCCGTATCTTCGACAACATGAAATTGCCGGAGCACGTCACCGAGAAGTCCGAATGGATGACGACC ACATGCAACCACGCCTTGTACGCTATTATTGATGTCTTCACGCAGTATTTCGATGTTCTTGGTCATCTGCTGCTGGAGGA GCTCTTCGCCCAGCTGCATTGGTGTGTTCAGCAGAGTAACGAGCAGTTGGCGCGATCTGGCACCAATTGCCTGGAGAACC TCGTCATTTCGAATGGATTCAAGTTCAACGAGTCCACCTGGGACAAGACGTGCCAGTGCATCCTGGACATCTTCAACGCC ACTTTGCCGCAGGATCTCCTCAGTTGGCGGCCGAAAGCACATTCCAGTAACAATATACCCCAGGAGCACAACCACTTTGA GGCGCTGCATATCCGCTGCGTAGTCCAGCTGGAACTGATACAGACCATGGATAACATTGTCTTTTTCCCGGCCACGTCGC GCAAGGAGGATGCCGAAACGCTGGCCCAGGCGGCGGCAGACTTAACAGGCGGCAGGAGCGGTTCGCAGTCGCAGCTGCTG GAGTGCCAGCGGGAGGAGCAGGGAATGTACGGCTATCTGAGAACCCGCCAGCTGCTCACCCTGGCCGACTGTCTGATGCA GTCGCACCGTTTTGCCAAGCGCTTCAACGCCGATCACGACCAACGCAGCCTGCTTTGGCGGGCGGGATTCAAGGGATCTG TTAAACCGAATCTGCTGAAGCAGGAGACCTCGTCGCTGGCCTGCGTCCTGCGCATTTTCTTCAAGATGTACGGCGACGAG AATAGACGCAGCGATTGGCCCGGCATCGAGCAGGAACTGGTGCAGGTCTGCAAGGAGGCACTGGGCTACTATTTGAGTTT GCAGAGCGAGGCACACCGAGATGCGTGGACATCGCTGCTGCTGCTCATCCTGACGCGCCTGCTCAAGATGTCCGATGCCA GGTTCGCCACCCACGTTTCCAACTACTACAGCCTGCTGTGCGAGATGATGTGCTTCGACCTCAAGCCCGAACTGAGAAGT GTCCTTAGGCGTGTGTTCATGCGCATCGGTCCAGTATTCAATATAATGAGCGTTAAATAGTCACGGAGGAGCCGCAGCCA CCTGGCTAGCTGACTCGGCCATGTTAATAGATCGATTCCCGATCCACCCGAAATCCCCTCAAATCGTAGTTCATAGTGGG CCCTCTGGTTCCCTCGGCTTTGTTGTTATGTTGGCACTTAATCATTCATACAAGTATATATATTTTTATTATATTTATAA ACAATACATAGTCAGAATGTAATGTTTACTAGGTTTAACGAGAAATGTTGTTGTTTTTATATATATATATAAATATATAT ATATATATATATGTATATGTATATGCATATGTACATACCCCTGGGTATGCTATGTAAGGCTTTAACGTGTGCGTGTGTAT AGTTTCCTTCAGTTACGAATTTGGATTTTGTTTCCCCGGTGTAACATTCACCCACTATCGATTAGTGTGTAGCGTAGATT AATGGATTAAACCAGCATTGTGCGCCGCCTGTATATTGCTAGTGTTAATTTAAATCAAAATCTATATATAAATATATATA TTTCAAATATACAATATAAATCTATTACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Cloned cDNAs prepared from polyadenylated RNA isolated from 0-22-hour embryos.
Embryos at 0-22 hr AEL were collected from an isogenic y; cn bw sp (iso-1) strain.
RNA was poly(A)+ selected twice. cDNA made using Stratagene ZAP-cDNA synthesis kit; oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. For clones LD21101 and higher, cDNA directionally cloned into EcoRI/XhoI-digested pOT2 plasmid.
