Comment: 0-22 hr AEL
ATCGAGTGTCACACGGGAAGAAGAAGCTGCGAAAAGAATTTTAGAAGAAAGCAACTGCAATTTTGTCATAATCGGAGCTG CAATTCGCAATGAATAATGGAAACTAGACAACCCAGTTCCGGTGATGATCTAAAGCCAGAGCTGTACTTCCTCATTTCCA AGTTCCTGGCCGCGGGACCGCTGGAGGAGACGGCGAAGGTCTTGATCCGGGAACTCGAAGAGAAGAAGGTTTTGCCGCGT CGTCTCGATTGGCAGGGAAACGAGCATGAGCAGACCTTTGAGGAATTGGAACGAAAGTACAAGCACATTGGCGCCAATCA CTTGCTTGAAATATGCAGTCGTCTGGGTCCACTGGTGGATCGCGAGCTTCCGCCCAGTGTTCCGGGCATCAACTCGCTGT TGGGTACTGGGCGCCAGAACCTCCTGCGCACCAAGGAAACCGTCTACTGCCATCGCTCCTTAAGGGACTACTGCACCCGC CTGAATGGAGTCTCGCTGCCGGATTCGGTCCTTACAAAGCCGACCCACAACTTAGATCGCGTTCTAACTGGACGGGAACA CGGCGGGGAAGTCCGTCGCAAGCTCCTCGTGCCCACGGACCTCTACCGCCGAACAAAATTGCTCCGTCGCACTGTGGGCC ACCTGTCCTCAGTGTACTGTGTTCTGTTTGATCGCACAGGAAGATACATCATCACCGGAGCCGATGATCTACTTATTAAG ATATGGTCAGCGGCAGATGGCCGCCTGCTGGCCACTCTGCGAGGCGCCTCATCCGAGATTACAGACATAGCCATTAACTT GGACAATACGATGCTGGCTGCCGGGTCATTGGATCACATACTAAGAGTGTGGGACATGCAAACCACATCACCCATCGCCG TACTGTCTGCTCACACGGGCATGATAACTTCGGTTAACTTTTGTCCGTCGCCACGTAGCGATCTGAAATATCTGGTGACT ACCAGCACAGATGGATCGATCGCCTTCTGGCAATACTCAACGCCGCGCGGGCAAAAGATCACGTTTGCCCCGAAGCCAAC GCAGTATCATGAGAAACTGCGGCCCGGACAAGCTCAGATGATGTGTACCACCTTTTCGCCAGGAGGCATTTTTCTGGCCG CTGGGTCTGCCGATCACCATGTGCGCGTCTATATGATGGGCGAGGACGGTCCGAAGCGAATATTGGAGACGGAAGCTTAC ACAGATGCCGTGGACTCGGTGCAGTGGTCGCATCGTGGACTGAGATTTATATCGGGGAGCAAGGACGGCACAGCACACAT CTGGACGTTTGAGTCGCAGCAGTGGAAAAGTTCCAAACTATGCATGACGGAGCGTCTGGCCAGCTGCCCGGAACCAGAGG AGGGAAAGAGGCTTAAGGTAACCATGGTGGCTTGGGATGCCTCGGACCGTTATGTCATCACCGCCGTAAACGATTTTACT ATAAAAATCTGGGACTCGAAGTCAGCCAAGCTGCATCGTGTTCTGCGTGGGCACAAGGATGAGCTTTATGTGCTTGAGTC GAACCCAAGAGATGAGCATGTCCTGCTTTCCGCCGGGCACGATGGCCAGGTATTTCTGTGGGACATCGAGCAGGGGCGTT TGTGTGGCCAACTTCCTTAACGACATCGATGGCCAAGGCCATGGCAGTGTCTTTGATGCAAAGTGGTCACCGGATGGCAC AATGATAGCTGCCACCGATTCGCATGGACACATCCTGATTTTTGGTTTGGGCGTGTGCATTGAAAAGTACAAAATGCTTC CCACGGAGCTGTTCTTTCACACGGATTTTCGACCTTTATTGAGAGATGCGCAGCACCATGTGGTGGACGAGCAGACGCAG ATAATGCCGCACCTAATGCCCCCGCCTTTCCTGGTCGATGCAGATGGCAACCCGCATCCATCAAGGTTTCAACGATTTGT GCCTGGTCGTGAGAGCTGTAGCCTAGACCAATTGATTCCAAATCTTACGGTAGGCGTGGATGGCGTAGTAATCGACGATG GAGGTGCAGGTGGAGATGCTGTTGTGGCCGCTGCACCCGCTGAAGCTCCGCCAGCCGCATTGCCCCCTGCTGCTGCTGGA GGACCACAGGGAGCTAACTACTCCCATATCGACCGCATGATTGCAGCCTTGGCCAATCGGCAGTCGGTTAATGTAAATGC CAATGATGCCAATCCGACCAACGGCAACCAGTCCTTCGAACGTTTGACCAATCGTCAAATGCCGGACTCCAATGGCAGGC AATCGTCACGTGGCAATGTCCTGGGTAGTCGTTTGAGCACGCCTCGCCAAGGATGGGGTGCCGCAGCTGCCGCGCCAGTT GAGGATCAAGCAGGGCCGTCTCAACCTCCGGCAGAACCTGCAGCCGAACCTGGCCAGGCACAAGTCCAACAGGCGCTGCC TCTGAAATTCATCCGTCGCACTTATGTGCGTCCCATGAAGTATCCCCAGTTGCAAAACCTTAAGCAGACTATTTACTCTG CCGGTCAGTTTGAAAAGCAGGAGTACAAACGCGAAATGCGACGCCGACCCATCATGATAAACACTGCCAGTGCAGCATCT TCGCAGCAGCCAGGAAGCGTTGGCAGGCCACGAAACACACGTGGCAATGGCGGCGGCGCAAGAGCTGGACGAAGACGTGG CCCCCAGCCAGCTGGCGGACAGCCACAACCCGCTTATCGCACCCGCGCTGTTCGGGATCAGGAGCCGGAGCACTACGATG AGGTAGCGCCGCCACCCGAGGAAGAGGAGGAAGATGTGTCCAGCAACTCGTCCGGCGACACGAGCTACTCGAATGTGGAA GAAAACCTAGAGGACAGCAGCGATGAGTCGGAAACGGACAGTTCCGATTACTCTGACTGGGTGGCGGATACTCCTGGCCC GAATCTGGAACCACCAAAACGATCCAAGCGTAAGCCACTCTCACGGCGACCCCGGAGTAGTGATGACAGCAGCGATGATG AGCGAGCGGGCAGATCCAGAGGTGGGCAGGTTACGGCCCGGAAGGTTGGACGTAAAACTGTTTTGTTTCCGCCCACAGAT GCAAATGGAGAGATACCGGAGCTTTATCGTCCCGCTGAGTGGCTATCTGAGGTGATTCCACGTAAGGCACCCTATTACCC CCAGATGGGTGATGAGGTAGTCTACTTCCGGCAAGGACACGCAAAGTACTTGGATGCAGTCCGCCTGAAAAAGGTCTACA AGCTCTCGCACAGCTCGGAGCCGTGGAACTTTCACACACTGCGCGACCACGAGCTGGTGCGTGTGATTGGAATCAAGTAC GAAATCCGCCCACCCCGATTATGTTGCTTGAAGTTGGCCATCATCGATGAGGAGGGCAACATGACGGGGACAACGTTCAA GATCAAGTACCATGACATGCCCGACGTTTTGGACTTTTTAGTGCTGCGGCAGACCTTCGATCTTGCAGTGCAGCGCAACT GGAGCATTGGTGACCGGTTTCGCTGCATGATTGTCGATGGCTGGTGGATGGGTCAGATTGAATCGCGCCACGCCCTCTCT GCTGACTTCCCCGACTCGTCCTTCATGTGTTTCCGCGTGCGCTGGGATAATGGAGAGTATGAGTTTATGAGTCCCTGGGA CATGGAACCCATCGATCAGCAAAGAGTACCGGACGAGGTGGGAGGTGCTGTTCCAGTTCTACCGGAGGAGATTCGCGCCA CTCTATACCAGCCCAAGTCCGAGGAATGGCACAGAGGGGATCGCGATGGCACCTGCAGGCGAATTATCAATGGATTGGAG CAGGTTATGCGTCTCTCTATCGCAGAGCATTTCCTGGCCCCCGTGGACCTTAACATATATCCAGACTACGCTTATTTGAT CGAATATCCCATTGACCTGACCACAATTAAATCTCGCTTTGAGAATCACTTCTACCGCAGAATAACCTCAGCCCAGTTCG ATGTACGCTACCTAGCCACCAATGCGGAGCAGTACAATCGCCGACACGCAATTATCGTCAAGCATTCGCGAATTGTGACA GATCTTTGTTTGAGGATCATCAGGGAACCGGATGAGATAGATGTGGCTGCTGTTTACCATCAGCTTGCGGATGTGTACCA TTCGTCCGAGTCGGAGAACGAGGTAGATTCGGATGTAGTGCCATCGACCAGCACAGGGCCAACTACTTCAGCAGCTGCAG CAGCAGCTAGGCAAAGAGTATCCTCAGCCAGGCGATCCACTCGAATTCACTCGGAAGGAGACTGGCGTGCCGATTGTCGT CAGCTGCTTGATCTTATGTGGGCGAGGACAGATTCGGCGCCGTTCCGCGAGCCCGTTGACACCATCGACTTCCCTGACTA CCTGGAGATCATAGCTACTCCAATGGATCTGCGCACTGTAAAGGAGGATCTGCTGGGCGGCAACTACGATGATCCGTTGG ACTTTGCCAAGGATGTGCGGCTGATCTTCCAAAACTCCAAGAACTACAACACCAACAAGCGCTCACGGATTTATGCCATG ACCTTGCGACTCAGTGCGCTATTTGAGTCGCATATCAAGACAGTGGTCACCAGCTGGAAGGCGGCATTAAGGCGCGCTAA TAAAAACAAGTCCGGCAGCAGTGGAGGAGGACGCGGTGGTTCCAGCAGCAGTCCCAACAAGCGAGTCAAAAAGGAGCGAT CCACGCGCCGTAATCAAAAGCCAACAGCAGCGGCCAGAAGATTGCCAGTCGCCAACTCCAGTGAAGATGAGGACGAGGAG GATGATGATGATGACGACGATGATGATGATGAGGAAGAGGCATCAACGAGGGGGCGCTCTCGTCGGCGAAACGGTGTGGC CGTAAGTGCTAGTTCCTCTGGCTCCAATCGCGTGACTTCTTCATCCGCCGCCCAACAGCGTCGAGGCAAAAGCAGCAGTA GCGAGGAGGAAGAGGAGGAGGAAACCAGTGCGCAGGCCACGTCGGATGCCAGCAGCGATGAAGAGGAAAGAGCCCAATCC AATGACAGTTCGCCCCAGCAGACACGCACGCGCAGGGCAAGGCAGCGGCAACGGCTGAGCGATGACTCCAATATCAACGA TTCCGAGGATAGTTACAACCCCAACGAGGGACGCGGCAGTCGGCGACGTCCAGGTGCCGCACGCCCGTCCAGCAATAGGC AGACAAATGGCCACTCCAGCAAGAGGCGACGTTTGAACAAAAGTCCAGCCAATGCTGCACCTGGCCCTAGTTTGCCACGT CGGACACGTCGCATAGCAGGCAGCTCCGCAGAAGAGGCTGCATCTCACTCACAACACGATGAGAGCACACAGGATAGCCA GATAGGACGTCGAAAGGGACGCTCGGCAAGCAGCAGGTCAATTGGCAATGGACCAAGTACCAGTGCCGCAGCTGCCGCCA ACTCCAGCCTTGAAAGTCCGTCACGGAATACTCGAGCCAACGGAACCAGGACCTCTTCGCATCTTGGAGCCGAAAATGAT CACAGTTACCACCTGCCAGTGCGCAACGGGCGCATTATCGAGTCCGATACGGACTCTCCTGTCATCAGACCCACGCGACA GAGCGCCAAGAGGGCGCTAATGGACTGGGGTCGTGGAAGCGATATTGAAGAGGAGGAGGAGGAAGAGGAAGAAGACAGCG AGGAAGCCGAGGAGATACTGCCAACACCTACAAAGGACGACTTGCCCTCTACCAGCCGAGCCGCTTTGGCTCAGGCGGCT GCTGGTCCTTCTCGCCAGCTACGCACGAACCGCAACACCGTCGTTCGTCCAGCCCAGAGTGACGACGATGACGACGACGA CAGTGACAACGAGCCGCTGGCCAACAATCAACAAAAAAATTATCAATCACCTGCAAAATCTAGCTCAACCGCAACAGTGG CCCCTCATCCACTGAGCCTGCGCCGTCGATTAGCCTCGCCGCCAGAACGGAATACCCATATGACCCGTTCCCATGCCACC TCCACAACGTCCTCGGCCGCAGCATCACGGGCAGAACAACAACCAGCGGTTTCCACCCACGATCACAACTACCTAGGCAA CGTGAATTCATCGAGGGCACCGCGCCGCATTCTTTCACGCCACCAACGAAATGCCGACGAGTTGGATACCAGTCTAGATG TCCTGGAAAACTCGCGTCTGCTCTCAGCCATAGCCAATCACCGGCGACCCACCTCTACCACGAACAGCAACAGTGCGAGC AACGTGGTTACAAGGCGTCTGCGTGGTCGCGCTAGCCAGGCGAATTCGCACGACGAGGATCATGAGGAAGAGGAGGCTGC TGCTTCCGATGAGGGCACTGATGGAGGCTCTAGCTCGCAGAGCTACGAAGATGAGGATGAAGACTCCGACTCGGAAGACA ATCAGCCGCTGACCAGCTACGTATCGCCCAGCAATGGTCGTCCGAGGCGAAAGGCAAAAACCTCCTCCTCCTCCGCAACA ACTCAGGAGCGTCCGCTGCGACGCACACGTCGCCCGCCATCCAATAACTCCATCGCGAACGACAACGATGACGATGATGA CTATGTTGTGCCGCGGAGTCGTCGCGCCAACCGGGGTGGAAACCAGCGAGCCGGGCGCAACCAAAAGAGACCGCGCTACA ATGAGCAGTCCGAGGAGGAAGAGGCGGCGGCTGGATATTCCCATCGGAGTAAACGAATCCGTAACGGCGACGCTCATGCC AGCTCTCAGGAGCGATCACAGGCCTCCACCGAGCGGGACCAGGAGGAAGAAGAGGACGGTACTTCGGTGGACTCGGAAGA ACAGCAGGTGAACGTTAGCAGCAGAGGCAGGGTGCGCAAGCCGTCTGCCAAGGCGCGCGGTATCTTCAAGGAGTGACCGC AGACTGTTTTATGTATAAATTAAATCTAATTGCTAAATCAACAAACCTTTGTAACATTTGCTAGAGTCTTATTTCTTATT ATTGATTAATATGTAAGAGTAAATCACGTCACTTCTCATTGTAATGTCAGTGTCACCGCTACGGTCATTGTCCCACACCC AACCCACCCCGATCCGTCAAATCCGTAACCCCAACCGTGAAGTGTTAGCCCATATCCATCATACACCGTGGCAGAATATG TTCAATTAATGTTCAAGTCTTAAGTTGAATATTGGCATCAGTCCTACATTCTATGTGTAGGGGGCATGGCCAAAGCAACG TTTTATATGTATTTCTATAATATATAGGTACTATCATACATATATATTTAAAGGTAGCACTCGGAATCACACTTCATTTA AAATAGTTTTAGAATAAAGTATCATGAAAAAAAAAAAAAAAAAA
Cloned cDNAs prepared from polyadenylated RNA isolated from 0-22-hour embryos.
Embryos at 0-22 hr AEL were collected from an isogenic y; cn bw sp (iso-1) strain.
RNA was poly(A)+ selected twice. cDNA made using Stratagene ZAP-cDNA synthesis kit; oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. For clones LD21101 and higher, cDNA directionally cloned into EcoRI/XhoI-digested pOT2 plasmid.
