Comment: 0-22 hr AEL
CGGAGCGTTCAGTCGACCGGACGCATGGTCGCTGATACAAGATACAAGATACACGATATAGATACCCGATACCCAGATAC TATATATGTGGTGCAGTGTGTTAGTGTTTTGGTGCGAGCAACTGTAAATTCGCAGCTGACCAAAGAAAGTGCGGTCGTCG CAATAAAACCGAATTAAATTCAATCGAAATCGGAATCGGATCCACACAGTTACAACTCAATTCGCGTTCGTCGCCTAAGT CTTTCGTTCAGCGCGCTTCGCTGCCACGCTGTTTTCGCACTCACGCCCGATTTTCGGTGGCAATTTCGAAACAAGCAGGG CGCATATCACTATCACAACGAGCTGCCAGACGGTCTAGGCCAAGAACTCCCCGCAGGTGTCCGGCAGTCCAGCAGCAAAC ATGTTTAAGTGGGTTACGCCGGCCTCAACGGCGACGTTGTCCCGCTGCACACTGCCAGCAACAACAGCAGCCACAACAAC AACAACGGCGATGGCAGCAACGAGAACAGCAACGACAACGACGAGAACAACAAGACCACAACTACTTTCCATCGCTTTAA CAAGTTTAATAATAATAGTCGCATCATTTGTGCCGACAACAAGTGGATTTAGATCAATTGAAACGAATGGCGGTGGTCGC AAGCTCTTTGGGGGCTATCGCATCACACCGAAGCACTGCCGTGCCACCAAGACGCTGCCCAGCTCGGATCCACGGGCCAA TGGACCCACCATCTGCATGTTTAACCACGAGTGCGCACAGCGGGGTGGCGAGGTGGTGGGCGCCTGCATGGACGGCTTCC TCTTCGGCGCCTGCTGCCAGATCCCGCCCACCCACGAGTTGGCCAGCACGCTGATTAACGAGGCGCAGAACGCCTACTTC CAGCAGCACCAGCAGCAGACGAAGCTCCAGCAGTCAGCAGCCCAATCCTCGTTCGAAAGCTACGGCGAACAGCAACAGTC ATTGAGCGAGGAGCAGGTGGCACAGCAGCCGTCGCAGAACATCTACGATCAGCAGAACCTGGACAAGGTGTACCAGCAAC TGGACAGCAGCAGTAGCATCAGTCCACCCAACGGAGCCTACGGCGATGAGCCGCAGCAGCAGGAGTATCAGTCGGAGTCG GAGCAGCCCGTAAGGGATGAGAATGCGTATCCTACCAGCAGTTCATCCACCGAGGCCACTCAGTCGCAGTCCTCCTCCGC CAGTGTGGAATTTGAGCAGGAGCCCTCCCAACCAGCAGATGCCTCCAACGATCAGACCACTCAGAAGATCAACAAACAGC CCGTCCAGCCACCCAACTTCCACGTCCACAAGCACTCCGTAACCATCAACTCACCCTCTTCGCCGCCTCAAAACGATGAC TTCGTAATGCAGGTGCTAAGCACCTTGCCACCTGAGCATGCGGATGACCATCATATTGTCTTTACCACCGAGGTGCCCAC CAAGATCACCAGTGGATTGCAGGATCAAACGAGCTCTGAGTCCAACTCCTTCGAAGAGGTGTCTTCGACGCCAGCAGCCA CCCAAAAACCGAAACCCAAGCCAACTCAAATGCCCACTCAGAAGACCACTCAGAAGGCCACGCAGAAGCCCACTCCAAAG CCCACACAGAAAGCCAAGCCGAAACCAGTTCCCCAACTGGCGGAGAGCATGAAGCGACCCATCCAACAGAAGCCACAGCA GGTGGCGAAACCTAAGCCAAGCCCTAAGCCTGCCCAGTCCACAAACAACCACCACCACAACCACTTGATCCTGGACGGTG GCGAGTTCACGCACAGTGACATCACCCACCCCGGAGCAGATGCCGATCTCGTGGAGGATCTGCAATTCTCCACTGGCTAT GGCCCCCAACCTGTGTACGCAGAACCACCGAAGCAGCAACAGCAACAGCAACCTGCTGAGCAGAGCTACATTTCCTCCAG CACTAGTGCGAAACGTCCAACGACAGGTCATAATAGTCCCACCACTGTTTCGTCAATTACCACCCACGTAGACTCTATCG AGTCCATCATCCTGCAGCTAAACAATACCAGTCATGGTCCCAGCTATAATGTTGTGAGCCAGCAGACCCCATCATATGGA TATCCTGGAGCAGCAGTGGTTCAGACTGAGCCAGCAGCCCAAAATCCGACCTTCTACCAGGAGAACGAGTCGGAGAAGGT CCAGGAGTCGGACTCGCAGTCTGATTACGGTTACACCACCACAGTTAACTACGAGTCTTTCTACGACAAGGTTTCAGATG AGCAGGATGCCTCCGCTGCTGTCAGTCAGTCTGCTGAGATGCCCACCGCACGTCCTGGATACGGAGAAGATGTTTCCGCT GTGCTGGAGGACCATACGATGCCGGCCAATGGTTACCACGATGCCGAGGCTCCAGTTGCTCCACAGACTTCGGAGTTTAA CAAGATGCCAGTGATGGGCATTGCCTATCCAGTGGATATGTCTTATATGGAGGAGGAGGGAAATCTTCCGGCCACTGCAG CTGGTTATGGCCAGATGAGCAGTGACTCCTACGAGGCGAGCACTGAATCCACCTACCAGAAGTTGTCAACGGTCCAAACG GAGGAGCCGCAGCCAACTTACGTGCGCCCCACAACCAATGCCAATAAGCAGAATCGCCCAGTGGCCTCCTACATTGGAAT GGTGACAATGCAGCACTATAACCCGCAGCCCGGAAATGGTGACTACCAGGCTCAAGTGCCCCCCGAAGTATCCGTGTCCT CGCACACCACCAAAGTCCAGGAGCAGATGGATGAGACCTCGAATGGCTATCAGCAATCGGAGACCACTTCTGGCTATGTT TCGCCACCAACTGCTGTTCCTGCGCCAGCACAGCGGCCTCAGTATGACGCTGTCCAGGGTGATGCTTCCTCGGAGCGACC TGTCCTGGTGACGGCCAGTCCCAGGCCCCGACCCAAGCCCAGTACCAAGCGACCTGCCGTGAAGAGACCCATCAGCGGCG AGAGCACCAAGAAGAAACCTCAGCCACAGCCAAGTGCCGGTGCCTACAACCAAGAGAAGATCAGCGAACATAGCACAAGA AAGCCAGTGTCCAATGGATACGATAAGGTACCCGAGTCGCCCATCACACACATTCAGATTAAGAAGCCATCGGCAACTCA ACACAAGGAACAAGAGCAGACGGGATACCCCAGACCTGCCAGCCCTGCGGGATACGAACAGACAACTGCCGCAGCTCCTG CGCCAGCTGCTCCTTCTCTCAACTACGACAAGCCGGATGCTCCTCCCAGCCAGTACGACCAGCCCTCGGCTCCATCCGCA AGTTACGATCAGTTGGCGCCGATGCCGTCTCTCAACTACAACGAGCAGCACGCTAGTTCACCAGGAAGGAAGCCGTCAAC GGCCAAGCCCATTTCCACCAGCTATGTGACCGGACCCAGTACCCCACGACCCCCAGCCACCGTTGATTACCACTATGACA ATGTGCCACCACTGTTCATGGCGGACGACAAGCTGGATGCCTTCATCCAGAGCACTGCGGAGAACATTGTCGGATCAACC CCGGGCAACTATCAGCCTCCGCTGGTGGCTACCGCATCGACGCCCGCTTACGCCCATAGACCAACCAGCAGTGGTAGCTA TGGCCATAAGAAGCCTGGCTTCGTCCAGATCAATGGAACACCCAAACCTCCCAGACCGACGGTGCTCATTACGCCCAAGC CCACTGCCATAAATCTTGTGACCTACAGCTCTTTGAGCGACGATAGCAACAAGCTGGCCTCCAGCACAAGTTCTTATGTG ACCGGAAGACCGGGAGTACAGGGAGTAAGTTCCAACGACTTCAAGGATCCCGGATACTTTGGCAGTAGCCCCGTCCATGT GGCATTCACGCAATCCACCACAGAGGCCGTCTACGCAGTGCCCTCCGATGACAAGCCAGCATTCCCAGGATACTTCGGGC CAACGCCCTCGTATCCCGCCTTCTCAGTTCCCGGCGAGAAAGTGGGCCAGAATGTTATGGAGGAAACCTACACGTCGCCC AATGATTTCGTCAACTTCCCGCCGGTAAGGAACCCCAATCTGAACATGTCGGCTGCCTCCAGCGCAGTGACCAGCGATCT CGACCTCTCCACACCCGCCTTTGTGGAGGATGTGGTGCTCAAGGACAAGATGCACACCCTGGTTCACAAATTGGTAGCCT CGCTGCAGGGTAACTTTGAGGCCCTGGCCGATATGATCGAAGAGCCGGGAAGCAACAAGACCGTGGCCACCTACCAAGCC GGAGCAGGAGGAACTGCCAAGCCTGTGAGAGTAGTAACCACACGGAAGCCTGTGAGGACAGCCACCACAACCAGGCCCAA GGTAACCACAAAGAAACCAGTCACCCGGGTCACGACAAAGGCTCCCAACAAGAAGACCTCCGCTGTCAGCACCACCACTC GCAAGCCCGCTACGCGTCGCACCACCGTTGCCGCAAAGGTAACCACCACCACTCGGAGACCGGCGACCAAGAAGCCAACT CGCAGGGTAAGCAGTACCGTGAAGACCACGACCGTTAGTTCGGCTAGACCGGCGGACGACGAGATCGTGGACGAGGAGGA CGAAGAGGACGTTAACCCGAATCCCAGCGACAACGAGATTGACCAAGGAGCTACTTTGAGCTCGTACGGCGGAGCCAACG GTCGGAAGATTCAGTGCGGTGTACGGCCCCATGTCAAGTCCGGCCGCATTGTCGGAGGCAAGGGCTCCACCTTCGGCGCC TACCCCTGGCAGGTCCTCGTCCGGGAGTCCACCTGGCTGGGCCTATTCACCAAGAACAAGTGCGGCGGCGTCCTAATCAC CAGCCGCTACGTCATCACCGCCGCCCATTGTCAGCCTGGATTCCTGGCCTCTCTGGTGGCTGTTATGGGCGAGTTTGACA TTTCCGGCGACCTGGAGAGCAAGCGTTCAGTGACCAAGAATGTCAAGCGCGTCATTGTCCACCGGCAGTACGATCCAGCC ACGTTTGAAAACGATCTGGCCCTGCTGGAACTGGACAGTCCAGTGCAATTCGATACCCATATAGTGCCCATTTGCATGCC CAATGATGTGGCGGACTTCACTGGACGCATGGCGACAGTGACGGGATGGGGACGCCTTAAGTACGGAGGTGGAGTTCCCT CCGTTCTCCAGGAGGTGCAGGTGCCGATTATCGAGAACAGCGTTTGCCAGGAGATGTTCCACACCGCTGGACACAACAAG AAGATTCTCACCTCCTTCCTGTGCGCCGGCTATGCCAACGGACAAAAGGACTCGTGCGAGGGCGACAGTGGCGGTCCGCT GGTGCTGCAACGACCGGATGGACGCTACGAGTTGGCCGGAACCGTTTCCCACGGCATCAAGTGTGCGGCGCCGTATCTGC CAGGTGTCTATATGCGCACCACCTTCTACAAGCCGTGGCTGCGCAGCATAACGGGCGTGAAATAGGTAGTCGCCTCCACA GTTTTCAAGTTTCTCCCCATCCCAGAGATGGCCAGCGCCAACTAACTGCTGAGGCAGGAGCACCAAGTGTAAATAGAACG TAGAGTTTTAAGAGTCGCGGCTAGAAGCGTGGGAAAGTTGGCTCATCCTTTGCGACGTCGCATCTTCGGACCCCTTCCTC GAACCCCCTCCATGCTACCATCATTTCATTAGCTTAATAGAAAATCAACTGCAGCATTTCAAACGACATTCGCCGGGTAT TTCCACATTCCGAGTTAGCGACATTTCGCTTGAGGATGACTGCAGATTTCGTTTTTTTGAGTACGCGTGCCTAACACTTG TAGAGTCTGAATGAGAATTACGCTGATTGCAAAACGTAGGGATAAGACATATTTATTCTAGTTCTAAGCCTATTTATTTG ACTATTTATTTATTTATGTGACGTGACGTGAGGAAGCGCGACGATTTGTGCGTTTGATTCAACTAAAAAAATTGTTATCT TCATAGGCAAATTGTTGTTATGTTCAAATGAGAGCGAGAGCAAATGTTTTTAGTTCAATTGAATTTACTAATTTATCGAT TTATATTATTAATTCTTTTATCTAAATATGTTAATTGTTAAGTGAACGAACCAATCGAAAAAAAATCGAAATAAACGACA TACATTTTAGTAAAAACAAAAAAAAAAAAAAAAAAAAAA
Cloned cDNAs prepared from polyadenylated RNA isolated from 0-22-hour embryos.
Embryos at 0-22 hr AEL were collected from an isogenic y; cn bw sp (iso-1) strain.
RNA was poly(A)+ selected twice. cDNA made using Stratagene ZAP-cDNA synthesis kit; oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. For clones LD21101 and higher, cDNA directionally cloned into EcoRI/XhoI-digested pOT2 plasmid.
