Comment: 0-22 hr AEL
AAACGGAACACTACAATTCGACAACAGTTCGGCTTTTCTTTTTGCACTATTTCGGATAAATATGGTTTAGGCCAGACGGA GCAATAACTAAGATGGAATACGAATCTTCCGAAATCAGTGATATAACCACAGGAAGCTGCAAGAAAAGGCTCACATTTCT GAAATGTATTCTAAGCGACACCACAAGCGATCAAAAATGGGCGGCCGAGTTCGGCGAGGACACGGAAGGACATCAGTTTT CGTTGGACTATCTGCTGGACACCTTCATCGTTCTCTATGACGAGTGCAGCAACTCCTCCCTGCGCAGGGAGAAGGGCGTC TCTGATTTCCTCAAACTATCCAAACCCTTCGTGCACATAGTGCGAAAGCTTCGACTAAGCCGAGATGACTTTGACATACT CAAGATCATCGGACGCGGTGCCTTTGGAGAAGTGTGCGTGGTTCAGATGATATCCACCGAAAAGGTGTATGCCATGAAGA TTCTGAACAAGTGGGAGATGCTGAAGAGGGCCGAGACGGCCTGCTTCCGCGAGGAGCGGGATGTGTTGGTTTTCGGCGAC CGGCAGTGGATCACCAACCTTCACTATGCCTTTCAAGACAATATTAACCTGTATCTGGTGATGGACTATTACTGCGGCGG AGACCTGCTTACATTGCTCAGCAAATTCGAGGACAAGCTGCCCGAGGACATGGCCAAGTTCTACATCACAGAGATGATCC TGGCAATCAATAGTATTCACCAGATTAGGTACGTTCACAGGGACATCAAGCCGGATAATGTACTGCTCGATAAGCGCGGT CACGTGCGCCTGGCTGACTTTGGATCCTGCCTGCGCCTGGACAAGGATGGCACCGTACAGTCCAACGTGGCCGTGGGCAC ACCCGACTACATTTCCCCGGAAATTTTGAGGGCCATGGAGGACGGAAAGGGACGCTACGGCACGGAGTGTGATTGGTGGT CACTCGGAGTGTGCATGTACGAGATGCTCTACGGAGAGACGCCCTTCTACGCAGAAAGTTTGGTGGAAACCTATGGCAAG ATCATGAACCACCAAAACTGTTTCAACTTACCGTCACAAGAAACCCTAAATTACAAGGTATCGGAGACGTCTCAGGACTT GCTCTGCAAACTGATATGTATTCCCGAGAACCGACTTGGTCAGAACGGCATCCAAGACTTCATGGACCACCCCTGGTTTG TGGGCATCGACTGGAAGAACATAAGGCAGGGACCAGCACCGTATGTGCCGGAAGTGTCCAGTCCAACAGATACCTCCAAC TTCGATGTGGACGACAATGACGTCCGGTTAACGGACTCCATTCCGCCGTCAGCCAATCCTGCCTTCTCTGGATTTCACTT GCCCTTCATCGGGTTTACCTTCTCGCTGACCAGCAGCTCCACCTTGGACTCGAAGAAGAACCAGAGCTCGGGCTTCGGAG ATGATACCTTGGACACCATAAGCAGTCCCCAGTTGGCCATTCTGCCGAGCAACAACTCGGAGACACCGGTGGACTCCGTT CAGCTGAAGGCACTAAACGATCAGCTTGCGGCTTTGAAGCAGGAAAAGGCAGAGTTGTCAAAGCAACACAACGAGGTTTT CGAACGGCTCAAAACTCAAGACTCTGAACTTCAGGACGCCATCTCTCAACGAAACATCGCCATGATGGAGTACTCGGAGG TCACTGAGAAACTCTCGGAGCTGCGAAATCAGAAGCAGAAGCTCTCCCGCCAAGTCAGGGACAAGGAGGAGGAGCTTGAT GGTGCCATGCAGAAGAACGACAGCCTGCGGAACGAACTGAGAAAGTCGGACAAAACGCGAAGGGAATTGGAGCTTCACAT TGAGGATGCTGTCATCGAGGCTGCCAAAGAAAAGAAGCTGCGGGAGCATGCGGAGGACTGCTGCAGGCAGCTGCAGATGG AGCTGCGGAAGGGCTCCTCGAGTGTGGAGACAACTATGCCACTCAGCATTTCCTCCGAGATGTCGTCATACGAGATCGAA CGCCTTGAACTCCAGTTCTCGGAAAAGCTAAGTCATCAGCAGACGCGCCACAATATGGAGTTGGAAGCACTGCGCGAGCA GTTCAGCGAGCTAGAGAACGCGAATTTGGCACTCACCAAGGAGTTGCAACAAACACAGGAAAGGCTGAAGTACACTCAAA TGGAGTCGATTACCGATTCCGCCGAAACGCTTTTGGAATTGAAGAAGCAGCATGATTTGGAGAAAAGCTCATGGTTCGAG GAAAAGCAGAGATTAAGCTCCGAGGTGAATCTCAAGTCAAAGAGTCTTAAAGAACTTCAAGCGGAGGACGACGAGATCTT CAAGGAGTTGCGAATGAAACGGGAGGCCATCACCTTGTGGGAGCGGCAAATGGCGGAGATTATTCAATGGGTGTCCGATG AGAAAGACGCGCGAGGCTACTTGCAGGCCTTGGCCACCAAAATGACGGAGGAGTTGGAGTATCTGAAGCATGTGGGTACC TTCAACAACAACGGTGTGGACAATAAGAACTGGAGGAACCGGCGCTCTCAGAAGCTGGACAAGATGGAGCTGCTCAACCT GCAGAGCGCGCTGCAAAGGGAGATTCAGGCCAAGAACATGATATCGGATGAGCTGAGTCAAACCAGATCGGACCTCATAT CCACACAGAAAGAGGTTCGAGACTACAAAAAGCGGTACGACTCCATACTGCACGATTTCCAGAAGAAGGAAACCGAACTG CGGGACTTGCAGAAGGGAGGCTTGGAATACTCCGAGTCGTTCCTCAACAAATCAACGCATCATGGGCTAAGCAGCGCCTT CTTCCGCGACATGTCAAAGAACTCCGAGATTATAGATTCTGCCGAGAGCTTTGGCAACGAATCCGGCGACAATTTCACTC CCAACTTCTTCCAGTCCGGCAATTCGGGCATGCTCTTCAACTACGAGCCAAAGTATGCGGGTAAGAACAACAAGGATCAT TCGTCCATGAAAGAAGCCTCGGTCAGCGATTTGTCCCGCGAGGAGAGTGACCAGCTGGTCAAGGAATCTCAGAAGAAAGT GCCCGGCAACACGGCGATCCATCAGTTCCTGGTGCGCACATTCAGTAGTCCCACAAAATGCAATCACTGCACATCGCTGA TGGTGGGGCTCACACGACAAGGCGTTGTGTGCGAGATCTGCGGATTCGCCTGTCACACGATCTGCTGCCAAAAGGTGCCC ACCACGTGCCCCGTGCCCATGGATCAGACGAAACGGCCGCTGGGAATCGATCCCACCAGGGGAATCGGCACGGCATACGA GGGCTACGTAAAGGTCCCCAAGTCGGGGGTGATTAAGCGAGGATGGATTCGGCAGTTCGTAGTTGTTTGCGACTTTAAGT TGTTCCTCTATGACATTTCGCCGGATCGGTGTGCATTGCCCAGCGTGAGCGTGTCCCAAGTGCTGGATATGAGGGATCCC GAGTTCTCGGTGGGTAGTGTGCGCGAAAGCGATGTCATCCATGCCGCCAAGAAAGATGTGCCATGTATTTTCAAGATAAA AACCGCCCTTATCGATGGCGGTCTTTCGCTGAACACCCTTATGCTCGCCGACAACGAGTCGGAGAAGTCCAAGTGGGTCA TTGCCCTGGGAGAACTACATCGAATATTGAAACGAAACAGCTTACCAAATACTGCTATATTTAAAGTTAACGAGATTCTG GACAATACCCTGTCTTTAATAAGAAACGCATTGTGTTCTGTCATCATATATCCAAATCAAATACTCTTGGGCACCGAAGA CGGCCTGTTCTACATTAATCTGGACCAGTACGAGATCGCTCGTATTGGCGAAAGCAAGAAGATTCTTCAGCTATGGTACA TTGAAGAGGAGCAGATCCTCGTCATTCTCTGCGGAAAGCAGCGCAACTTGCGTTTATTACCTATAAGGGCATTAGAGGCT AGTGATGTCGAGTGGATCAAGGTGGTCGAATCGAAGAACTGCATATCTGCTTGCACTGGAATAATCCGCCGCTTCCCCAA TATCGTTTACTCATTTATCATCGCTCTAAAGCGGCCGAATAACCACACACAAATCGTAGTTTACGAAATCAATAGAACGC GCACAAGACACCAGAAGACTTGCGAATTCACAATTGGCTACATGGCGCAGCACCTGCAGATTCTGTCCGACATGCGATTG GTTGTGGCTCACCAAAGCGGATTTACCGCATACTTTCTGCGCGGAGAAGCAACTGCAATGTCTTTGGTTCATCCGGAGAA CCAGTTATGTGCATTTTTGAACTACTCCGGAGTGGATGCGGTCAGGGTCATTGAAATTCTGTGCCCCAGCGGCGGCAATT TCGGGGAGTACTTACTGGTGTTCCAGACTCTGGCCATATACGTAGACCTGCAAGGGCGAAAGTCTCGGGATAGGGAAATT ATGTATCCAGCCTTCCCTACGTATATAACCTTTTGTGATGGTCACTTGCTGGTGTTTTCGGATACACATTTGGATATATT CAATACGCAGACTGCCGAATGGGTGCAGTCGATTGGATTGAAGCAATCACTTCCCTTAAACAATTTGGGAAACGTGGTGC TGTCCTCAGTCAATGACACTCCGCTGATTGTTTACCTATCCAATATTCACACAAAGGGCCTTTTGCAATACCGTGACGGA AATCGAAAGGGACTTCCGAGCATAAAGCGGAGATTCTCTATCAGGGAGATAAACAAGACCATCAAAAGTGATCGTAGATC AAAGATGATATCAGCACCGACTAATTTCAACCACATTTCTCACATGGGGCCAGGGGATGGCATTCAAAATCAACGACTCC TAGACTTACCAACCACGCTCGAAACAGCCGATCAAGCCTGCAGTCCAATAATACATTCGCTGAGTTGCATACCCCAGAGT AGAAAATCCAATTTTCTAGAACAAGTGGATGCAAACTCCGATGACTATGGCAATGACAACATTATCTCCAGAACGCCCAG TCCAATGGCATCGTCCTTCATGGACGGCTTAAGCAATAATGATTAACTTCAGTCAATCTCTACACGTGTAATTTAATCGA TTTTTTAAGATAACTGTTTGTAATAAACATATTTAATTTCTCGAATATCAAAAAAAAAAAAAAAAAAAAAAAAA
Cloned cDNAs prepared from polyadenylated RNA isolated from 0-22-hour embryos.
Embryos at 0-22 hr AEL were collected from an isogenic y; cn bw sp (iso-1) strain.
RNA was poly(A)+ selected twice. cDNA made using Stratagene ZAP-cDNA synthesis kit; oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. For clones LD21101 and higher, cDNA directionally cloned into EcoRI/XhoI-digested pOT2 plasmid.
