Comment: 0-22 hr AEL
TTTTTGCAACGCAACGGGCTGGCGAATCGCTGCTGAAATTAAGCTAAAAACCGTGTAATAAACCGCAGGCCGTGGCAAAA CCAGCTCACCGATGCCGAGACGCAGCAAAATGTGATGTTTGCCGGGTGCTGCAAACGCTCGGCGACCGCAAACGAAGTGC AAGACCTAAAACCTCTGACCCCAAGCGCGTCACCCAGCAGAAGGATAGAGCGAGACGGACAGATAGGAGTGACTGAGGCA GGGAGAAAAAACTAGCACTGGCCCCAAGACGTTGGAATTTTGTGAATTGTTGTTGCTGCAGCCCAGCAATAACGGTACAG AGGACAACAGTATCAGCAACAAATACAACAAAAAGGAATGACAAAGTGAAACCGACTGCGCTGCCCCACAAACTACGACA ACATTAATAACAATAATAACAAAACGAAATAGGAAGAGCAAAACTGTGATCTCTGCTTAACTTTTTTTATTTTTGGGGCA ATTGTTCAATTTGGCTGTGCTCAAAAGTAAATTAAGTCAACTCGTTACGCGTATTTGCGGTGTTTGGCAACGCTTTTTCC AACCGACGACTGGAAAATCAATTCTTCGGATTGCCAAAGGGGAACAACAACTAGCAGTTGTTGAAGTTTTCCTTTATATT TTTTGCGGCCCAGCCCAAAACAAAAAGCAAAGTAAAAGAAGAAGAGAAAGAGGAAGAAGAAGAAGCAGCGCAGCCGGATT TGCAAATAGTTGCCGCAGAACGAAGGACGAATAGACATTGCAACGATGGCCTATCGGAAGCCCAGCGACCTGGATGGCTT TATCCAGCAAATGCCCAAGGCGGACATGCGTGTGAAGGTACAGCTCGCCGAGGATCTGGTGACATTCCTTAGCGACGACA CAAACTCAATTGTCTGCACGGACATGGGATTCCTTATTGACGGTTTGATGCCATGGCTGACGGGCAGCCACTTTAAGATT GCACAAAAGTCCCTGGAGGCGTTCTCGGAGCTAATAAAGCGATTGGGCAGCGATTTTAATGCATACACGGCTACCGTTCT GCCACATGTGATCGATCGGCTGGGAGACAGCAGGGACACAGTCCGCGAGAAGGCGCAACTTCTGCTGCGCGACCTCATGG AGCACAGAGTGCTTCCGCCCCAGGCGCTGATCGACAAGCTGGCCACTAGCTGCTTCAAGCACAAGAACGCCAAGGTGCGC GAGGAGTTCCTTCAGACGATTGTGAACGCTCTTCATGAGTACGGCACCCAGCAGCTTAGTGTTCGCGTCTATATACCACC AGTTTGTGCACTTCTCGGAGATCCCACAGTTAATGTGAGGGAGGCGGCCATCCAAACGCTAGTGGAAATCTACAAGCATG TAGGGGATCGATTGCGCCCAGACCTCCGTCGCATGGACGATGTTCCTGCCTCGAAATTGGCTATGTTGGAGCAAAAGTTC GACCAGGTCAAACAGGAGGGTCTACTGCTACCTTCAGCCCTTAAAAACACGAATGGCAATGGAGTGGGCTTGGACGAGGC CGACAATATTGGGTTGAGGGAGCGACCCACCAGGATGATTAAGCGGCCACTACACTCGGCCGTTTCGTCATCACTGCGCC CAAAACCCAATGTGAACGATGTGACCGGTGATGCCGGCGCCGTAACCATGGAATCTTTCGAATCTAGCTTTGAGGTGGTC CCGCAATTGAACATCTTCCACGCTAAGGACATGGACGATATCTACAAGCAAGTACTAGTGATCATCAGTGATAAAAACGC AGACTGGGAGAAACGTGTGGATGCTCTCAAGAAGATCAGGGCATTGCTCATTCTCAGCTATCACACTCAGCCGCAGTTTG TCGCTGTACAGCTAAAGGAATTGTCGTTAAGCTTCGTGGACATCCTCAAGGAGGAACTACGATCACAGGTGATCCGCGAG GCGTGCATCACCATCGCCTACATGTCTAAGACGCTGAGAAATAAACTAGATGCCTTCTGCTGGAGCATTTTGGAGCACCT GATTAATTTAATACAGAACAGCGCGAAGGTCATTGCATCCGCTTCCACAATAGCTCTGAAGTATATCATTAAGTATACAC ATGCACCAAAGCTGCTCAAGATCTACACAGACACTCTGAATCAATCAAAGTCGAAGGACATAAGGTCCACACTGTGTGAG CTGATGGTGCTGCTCTTCGAGGAGTGGCAGACGAAGGCGTTGGAAAGGAATGCCACCGTACTAAGGGACACCTTAAAAAA ATCCATTGGCGATGCAGACTGCGATGCACGCCGCCATTCCAGATACGCCTATTGGGCTTTCAGGCGTCACTTTCCAGAGC TGGCGGATCAAATATATGGAACATTAGACATAGCTGCCCAGCGCGCATTAGAAAGGGAACGAGAGGGCGGCGGAGGAGGA GGAACTGGTACTGGGACTGGGACTGCACCTGAAACGAGACGCACTGTATCCCGCATTGGCCGAACACCTGGAACCCTGCA AAAGCCCACGCCTAGTATGAGATCCATTTCAGCGGTGGACACTGCGGCTGCTCAACGAGCCAAAGTTAGAGCGCAATATA CACTATATTCCAGGCAAAGGAAACCTTTAGGACCTAATAATTCCAACCAGGCATCGATGACAGGAGCAGCAGCCAGTGGA TCACTGCCCAGACCTCGCCTGAATTCCAATAGCGGTGGCACACCAGCTACAACGCCGGGATCGGTTACACCACGGCCCCG GGGACGGGCAGGAGTATCTCAGTCACAGCCAGGATCGAGATCCACCTCGCCAAGCACAAAACTGAGGGATCAGTACGGTG GTATTGGTAATTACTATCGTGGGGCCACTGGCGCCATACCCAAAAAGGCCTCCGGAATACCCCGAAGCACAGCCAGCTCT AGGGAAACGAGTCCAACTAGGTCAGGTGGTGGCTTGATGAAACGCAGTATGTACTCTACAGGTGCGGGGTCTCGACGTAC GCCCGAGAGGAACAACCCAGTAAGACCATCGGCGGCGGCACGACTGCTGGCGCAATCCCGTGAAGCAGAACATACATTAG GCGTCGGAGATGATGGACAGCCAGACTATGTTTCCGGGGACTACATGCGCAGCGGCGGAATGCGGATGGGTAGGAAGCTC ATGGGACGTGATGAGTCTGATGACATCGACTCCGAGGCCAGTTCTGTGTGTTCAGAACGATCTTTCGACTCCAGCTACAC TAGAGGTAATAAATCGAACTACTCACTTAGCGGGAGCCACACCCGCTTGGACTGGAGCACGCAGCGGGCGCCATTTGACG ACATCGAGACGATTATTCAGTTCTGCGCATCGACGCATTGGTCTGAAAGGAAGGATGGCCTGATCAGCCTCACACAGTAT CTGGCCGATGGAAAGGAGCTCACTCAGCAGCAACTTAAATGCGTTCTGGACATGTTCCGCAAGATGTTCATGGATACACA CACCAAGGTGTACTCACTGTTTTTGGACACGGTCACCGAACTGATTCTGGTTCATGCGAACGAACTTCACGAATGGCTCT TCATCTTGTTGACGCGGTTGTTCAACAAACTGGGCACCGATCTACTCAATTCGATGCACAGCAAGATTTGGAAGACGCTA CAGGTGGTCCACGAATATTTCCCCACGCAGTTGCAGCTTAAAGAACTATTTAGAATCATATCGGATTCTACTCAAACGCC AACTACCAAGACGCGCATCGCCATTCTGCGCTTCCTTACGGATCTGGCCAATACATACTGCAAAAGCAGCGACTTTCCCA GCGACCAGAGCCAGGCCTGCGAGCGGACGGTCCTAAAGCTGGCCCAGCTGGCGGCGGATCAGAAGTCGATGGAGCTGCGC TCCCAGGCCAGGAGCTGCCTAGTGGCCCTGTATAACCTGAATACCCCGCAAATGACCCTTTTACTGGCCGACCTGCCAAA GGTATATCAGGACTCTGCCCGATCCTGCATCCATTCGCACATGAGGCGGCAAAGCCAAAGTTGCAATTCGGGTGCCAATT CGCCTAGTAGCTCTCCATTGAGCAGTAGCAGTCCCAAGCCTTTGCAAAGTCCCTCTGTGGGTCCATTTGCCTCGCTTCAG AGCCACCACCACCAACTTAGCATCAGCTCTACTAGTCCACGCTCCCGGCAGTCTTCCGTGGAGCAGGAGCTGCTCTTTTC CTCGGAGCTGGACATTCAGCACAACATTCAGAAGACGTCGGAGGAGATCCGGCACTGCTTCGGCGGTCAGTACCAGACGG CGCTGGCGCCCAATGGCTTCAATGGACACTTGCAGTATCACGATCAGGGCCAACAGGATTCGTGTGCATCCCTGTCTTCC AACTCCAAGACGCAATCGTCGGCCAACACTACCCAGTCAAATACACCTGAGTCAGCAACAATGAGGCTGGATAATCTGGA GCGGGAAAGGACCACTCAGAACGCCAAGTCACCAACTGACGATGCCAAGGTGATCACGGTCTCGATAAATATGGCTGAAA ATGGAGAGCTGATACTGGCCAGCAACCTGATGGAGAGCGAAGTGGTGCGTGTGGCCTTGACGCTAACAAAGGATCAGCCC GTCGAGTTGCTTCAGACGTCACTTACTAACCTGGGGATTTGCATCAAGGGCGGAAACTGTGAGCTGCCCAATAAGCACTT TAGATCGATCATGCGAATGCTGCTTAACATTCTGGAGGCGGAGCATACGGACGTGGTCATCGCTGGCCTGCACGTGCTCA GTAAGATTATGAGGAGCAACAAAATGCGTCACAACTGGATGCACTTTCTAGAGCTGATTTTGCTGAAGATCATCCAGTGC TATCAACACAGCAAGGAGGCTTTGCGGGATATCGACTCGATGATACCAAGGATAGCACCATCCTTGCCTCTGGATCTGTC CATCAACATTGTCAATCCGGTAATTGCGACGGGTGAGTTTCCCACAAATTTGTGCGCCATCAAGATCCTGCTGGAGGTGA CCGAACACCATGGTTCGGAGATCACAGACGCCCACTTGGACATTGTGTTCCCCAATCTGGCGCGATCGGCGGACGACACG CAATCGATGGTTCGCAAAGCGGCGGTCTTCTGCATCGTAAAGCTGTACTTTGTTCTGGGCGAGGAGAAGGTCAAGCCGAA GCTGTCAGTGCTAAATCCCAGCAAGGTTAGGCTCCTCAACGTGTACATCGAGAAGCAGCGGAACTGCATCAGTGGGGGAG GAAGCTCTACCAAGAACTCCTCCGCGGCATCGTCGTCATGATTGCGGGAGCCCTTAATAGGATTCCTGCATCAGCACCAC CACAAACAAGACACAGACGCGGTTGCTTCCCTCGGCCTGAGAAGGAAGTGAGGAGCGGCGGACATTAAATAATATATTAT TACATTAACTAATTATTTATAACTAATTATTACCGATCATCCGTTACTTGTGTAACTGCTCGATGCATATATTCAGCAGA TGCAAATGCGGCCCGAAAGAAAGTCAAGGGCCATCGCCCATCTAATGTGAGCAGAAAAACTATTTATACATAAACGGGAA CTAAAGCGAGTAAATCCGCAAAGTGTACTAAAATTGTAGCCAACTCCGCAATCCTCACTTCTCACATCAGTTGTACGTCT TTTTACCAGCTCCTAACTATTAACGCTGATTCTGTTTAATTTGTAAGCCTATATACCGCTCTTTATGGAAACTCAGACGT GTGCTTTTCTACCTTTGTTTCAGGCGACTTTGTATCCTTATTTCGTTTCCGCTCAACTAAATTCTAATTACTATTATTAA TACGATTCTGCTCTTACAACTGAACTATTTTTGTAATTAATTTAAATACACAAGCCCACACAAAGGATTAAAATCAATAA AAAACACTTATAGGTTATGTAATAATATTTATAAATTAATTAAACGAATAAAACAGTTAAACAAAAGTCCCAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Cloned cDNAs prepared from polyadenylated RNA isolated from 0-22-hour embryos.
Embryos at 0-22 hr AEL were collected from an isogenic y; cn bw sp (iso-1) strain.
RNA was poly(A)+ selected twice. cDNA made using Stratagene ZAP-cDNA synthesis kit; oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. For clones LD21101 and higher, cDNA directionally cloned into EcoRI/XhoI-digested pOT2 plasmid.
