Open Close
General Information
Symbol
Dmel\LD47466
Species
D. melanogaster
Name
LD47466
FlyBase ID
FBcl0177933
Feature type
Computed gene(s)
 
Collection Status
DGC gold BDGP
Known Problems
none
FlyBase assessment
    Library
    LD_pOT2_cDNA
    • LD cDNA library of D. melanogaster, iso-1 strain, embryo (0-22 hr), pOT2 vector.
    Strain
      Vector
      Tissue Source
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      Sequence Data of the Insert
      Full Length Sequence
      Total bases
      3,762
      NCBI
      Full Length sequence dataSequence Downloader
      CCGACTCGCGAGCACGCGCTGTGCTGCTTCTTCCTGCGTTGCGCGCTTTGCTTTCAATTCGCCTTTCGTCGTCGCGGGTG
      CGTGTGTGTTGGAGCGAGTGCGTCTATGTGCAGTGCGTGTGCCGCTATTTGTTAATTGTTAATAATCCCGATTTAATAAC
      ACCCGACAACCATGTTGCACTTCAATTGCTTCTGAACGCAAAATGCGCACTGATAAACGCAAACGACAAAAATAAGTTTC
      ATAATAATAATAATGCCGGCAAACAAAGCGGTGTATTAGTGTGCGTATTTCAGTGTGGGTGCCTGCGTTTGCCAAAATTA
      TTTACCGCAAAAAGAACAAACACAGTCAAAACGATTTGAATCCCAATCGAAACTAAACGACAACAAACGCCGGTGAAATT
      AGCCCTGTCTCTCGCTCTCTCTTTCCCCTTAAAATAAAGAAAGAGTTATCAAAATGCTACATCAGCAGCAACAACAACAA
      CATCTGCATTGCAGAAGAAAAGCTACAGCAACAACAACTGCCCGCCTTGTGATATTCAGCAGTCCTCTTCTACTATTACT
      ACTCACCACCCACTTGCCGCCCACCTTACAAGCGGACAATGGCCCCGCCCCCCAAGTGGCCGCCCTTGCCGCCCCCAATC
      CGGCGGGTGGCGTAGCAGGGTCTTCGATCATCGATCAATTCAGTCCGAATTGCAGTGCTGTGACACACATTTTCCAAGCG
      AGAGGAATCGATGCCATCGAAATTCCCCAAAAACCCAGTAACGAACGAGTCCTACGATATTGCGAGTCCCCTTCGGTGGG
      CACTTGTTGCACCTACAACATGGAGACCCGCATGGCGATGCAATCCCGCCAGCAGCTCGAAGGGCACACCAAGGACCAGA
      TCTCTCGTATGTCTGGAATCCTGGGCAGCAAGGCCACAAAATTCAAAGATATTTTCACGGCCCTGCTCAAAGAGTCGCGA
      ACGCAGTTCAATAGTATGTTCATCCGAACATACGGCGTCATTTACGAGAGAAATTCGTACGTCTTCTCGGATCTGTTCAA
      GGAGTTGGAAACCTACTTCGCCAACGGTCGCGTGGACTTGCTGGAGGTCATGGACAAGTTCTTCAATACGCTATACCAGA
      AGATGTTCACCGTGCTGAACACCCAGTACACATTTGATGAAAACTACATGCGATGCGTCAGCGAGCACATGAAGGAGCTG
      AAGCCTTTCGGAGATGTTCCGGACAAGCTGTCCGTGCAAATCAAGCGATCCTTTGTGGCCACGCGCACCTATGGCCAGGC
      TCTGACCACCGCTTCGGAGGTGGCGAAGAAGGTCCTCAATGTGCGACTGAATGCCGATTGTACTGGGGCTCTTACCAAGA
      TGCAGCACTGTGGTGCTTGCAAGGGATATACGGAAAAGCCCTGTACCAACTACTGTGTGAACGTCATCAAGGGATGCCTG
      CACTATCAGCACGAGTTCGACAGCGAATGGGAGAACTTTGCGATGGCCATGGACAAGGTGGCGGAACGACTGCTGGGATC
      CTTCAACATTGTGATGGTGGTGGAGCCACTCAACATCAAGATCTCCGAGGCCATCATGAACTTCCAGGATTCTGGCCAGG
      ACATCACCAACCGCGTATTCCAAGGATGCGGTAGGCCAAAGTTGAAGAAAATGAAACGGTCCATCAGCCCCAAACTGCAA
      GGAGTTCAAATCCTCAACGCAAGGAGTCCTGTGGAGGCCGATACTCTGGACATTGATGAAACGTTGGACGAGGCGATTGT
      GCTGAGAGAACGCAGAGCTGCTGAGCCCGGCTCACAGGAGACATCGGCGCAACAGTCTCAAGAGCAAGGAGTTGGAAAGA
      GTGGAAATGGAGGAGGAGGCGGTGGCGGTAACAACAGGCGGCAACAGCAACGTCGAAAGCAGCAGCAACAGCGACGCAAA
      CAGCAAAACAATCGCGACGACAACGATGACGATGACAATGAGAGCGGAGGTGGACGTGAACCGATTCTGGACAGGATCGT
      GAGGGACATTCGCCAGCGAGTGAAGGACTACAAAAAGTTCTGGTCCAATCTGCCGCACTCGGTTTGTAGCAACGAGGATA
      TAGCCTCCAGTTCCGATGTGGACGGCATGTGCTGGAATGGTCACACAATTGATAGATACATGCACTCAATCACCACAGAA
      CACGGCTCGAATCCGGAGTTTACAGGCAATCCTGCCAGTACTAAGCAAACAGCTCAAATGGCCTCTCAACTAAGTCATCT
      GAAGAATGCCATTGTCCACCTTCGAAATGCCTACAACGGCCAGGATGTTGAGTGGAGCGAACAAGAAGAACTGCCATATG
      CGGGTAGTGGAGCTGGTTCTGGATCCGGGTCCGAAGACGACGAGGACGATGACGAGGGCTCTGGCCTGGGACCCTTCGAA
      CCCAGCCATAAGCCGGACGTGGAGCGTCCTTCGGTGGATGCAGACAACGACGATGACGAGGACGCCGGTGGACGGGGTCA
      CATGCCCACGCACACATCTCGACCGACGAGCGGAGTGGACGACAAGAATCCGCTCATCCACACCACTCACTTCGACCAAG
      ATCACAACGATCTGGATGAGGATCATAGGCAGCTGGATGAGGATGAGGACACGGATGCAGGCCATGACGGGGCCAATGAC
      AATCGATCATCCGATGCCCCAGAAAAGATGACGCTGCGTCGCGCTCTGGTTGTTTATCTGCTGCCCCTTTATATGGCGTG
      GTTCGGTGGCGTCTGCGCCGATTTGCTGTGATTACTCAACGTGCGAAATGCAGCCAAGATGGAGCACGGCTGCATCCCCT
      ACACCTCTCAGTCATTCATCCGTGAAGCAAAATGTTATTAGCAGAACTACATGCTACCCCTTATATACGAAATGCGAGTC
      CAAAATTTTATAAAAACTTTTGATAGTAGCAATCAAAACGCAATCAAGAAAATAGGTAATTTTTCGAATTTTACTCTCGT
      CAGGAATTTTTTCAAATTTGAATTGGATGAAATCTCACTCTAAATCTAAAATTTAATTTTTGAAAACTACCAGATATTGT
      AAAAATCAGAAAATGCCTGATCTAAAGCGGTTCTTTAATGAGACCCGATAGGAGAAAAAGAAATTAGTGGAGGAGAGAGA
      ATACGTAACGAGCCCATTTTAGTTTAGTAAGCCCCCAAACGCCCCTGTTCCCCCACACTCTAAGTCTAAGTGCGAAATTG
      TATTTTGTAAGCCAGAGAGTGAATGAATGTAGAAGTGAGTGCGTGTATGTCGCGGAGTGAATGCTTTTACCGAGTGTGAG
      TGCGAGCGGAGTGCCTGTTCAATGTGATCGTTTAGCAAAAATTAACTATAAACACTTTAGCCCCTAGAGACCTAAGCGAA
      AAACACACCTACACCTACATATTTAATTTGTATTATATTTATGCATACGCGCATATGTAATGTAACCAGCAAATTGCCAC
      AGCACACAATTTCAATTATCACCGAAATGAAGAGCAATAACTAATTTGTAACTGTAGTCTACTGATAAACCTAAAAACTG
      AAGTCTTATTTACGTTAACATGGCCATAGAAAAGCGTCCGAGCCACGGCTTTTGTAATTTTGTATCTTTCGTAGACAGCC
      CTGACTTTTGCCAGCCATCAAACGCATTTCAAACTAAACTGTGTACACCTACATATTTATATTTATTTTTGATTTTTTAT
      CGGTTTTTATATATTGTATTAAAACAAATGGAAAAAGAAAAAATACAAAAACGTAACAAAAGAAAAAAAAAAAAAAAAAA
      AA
      
      Library Information (1)
      Library: LD_pOT2_cDNA
      Description

      Cloned cDNAs prepared from polyadenylated RNA isolated from 0-22-hour embryos.

      Sample preparation

      Embryos at 0-22 hr AEL were collected from an isogenic y; cn bw sp (iso-1) strain.

      Protocol

      RNA was poly(A)+ selected twice. cDNA made using Stratagene ZAP-cDNA synthesis kit; oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. For clones LD21101 and higher, cDNA directionally cloned into EcoRI/XhoI-digested pOT2 plasmid.

      Comment
      Reported As
      Symbol Synonyms
      LD_pOT2_cDNA
      References (1)
      Review (1)
      Rubin et al., 2000, Science 287(5461): 2222--2224
      A Drosophila complementary DNA resource. [FBrf0127297]
      External Crossreferences and Linkouts ( 2 )
      Crossreferences
      Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
      Linkouts
      DGRC - Stock center for Drosophila cDNAs, vectors, and cell lines
      Synonyms and Secondary IDs (0)
      Reported As
      Symbol Synonym
      Secondary FlyBase IDs
        References (0)