Comment: 0-22 hr AEL
ATTTCCACCCAACAGCAGCAGAATCAGCTGGCCCCCCAGCAGCGCCCGCCCAGCAGCTGTCTCTCATCCTCCTCGGCGGC CACCACGCTCTCTCAGTCGCTCTCCAACCGCACCTCGTACTGCAGCGTCGACGGCGGCAGCGCACGAAGTTTTTGCGGCG ACGAAGCTTTCGCAGCGGGACGATCGACGACGACGACGGCGGCAGCAGCAGCAGCGGCGGCGCTCTCAGTTCAGAAAACC ACCTCGCCGCGTCCACACGCATCGCTCTCGTCGCTCTGCACGGTGGAGAATTTCCGCGGCTCTGTTTCCTCGAACTCATC GATCGCCTCTCCCAAGTCGTGCAAGTCATCGGTTACCAAGACCTCTGCTGGCAAATCGCGCGTTTTGAAATCGCCAAAAT CCCCAAAATCACCAAAATCGCCGCTAAATCGCAAGGCGCACACCCCAAACAAATGGGATGCCGTGATGAATAAGATCGCT AGCAATAAGTCTCTGATCAAGACAAACTACAACGATGTAAAGTCAAAGGTCTCGACCACACGGATCATGACCCCGGCTTC CGGTTCGAGTCCGGTTTCCGGTTCCGGTTCGGGCTCCGTTTCGCGTACCGCTTCGAGTCCGCGCGCCAGTCCCAGTGTGA GTGCCAGTGCCCGGCGTTCGCCCTCGGCCACGCCCAAAGTGCCGCCCAAGATGCTTCTGGCCAAGCGACCGAGCAGCAGC AGCGGCAAGGTCAATGCCACGCCCTCGCCGCCCCCACACGCGACCCCGCCAACACGGCAGCCACTGGATAAAGGCTCAGT TGGCGCCGGGTCGGTGGGTGGTGGTGGCGCTCGTGCGGTAAAGACACCCAAGTCGCCCACCTCCCCCACCACACCTACCA CGCCCACATCGCCGCCTGCGAAGAGACTCCAGACGAGCCTCGTCAGTCGTGGACGCTCGTACAGCAAGGACAGCCACAAG AGTTCGCACACCGACTTGAGTTTAATGTGCAATGCCTCATCGTCCCGGCCATCGAACTCTGCGTCTGGAAGCGGATCGCC GAAGCTATTGGCAAACACTCAATTGCGAGCTGCCAAAAAGCGCGATGTGCGCAATCTGAGCATATCGCCAACCGATCTGG GTCCGCCACCAAAAACCCAACAGACAACCAAGGGACAATCGACGCGCAGCAAATCATCGGCCACGCCCACGCCAACGCCC ACATCCATACATAAACGTTTAAATGGCACTTTGGCCAGCACGGCAGCAATTGCAACACCCATCAAAGGAGTGGCCACCAA ATCCGGAGCCACAAACAAGCTGCAACAATCGGCAAAAACTAAATCAACCGCGAGTATCAAAAGTCCAGCAATTGTCGGCG TTTCCGAGGAATCCCTGCGCTCTGGCGTGGATCAAACCGAACTTCAGGAGCTGAACAATGGGCAATCTGCTACCGGTTGT CCGACGCCGCCGATGGCGCGGGACTCAAAGCGTACGTCCATTGGAAATGAGTTACCCTGTGAGACGGAGGCCAAGTTGAA GAGATGCGAGGACCAGCAGGATCCACAAGCATCCACGACGGCATCACCCCTACCGATGATCAATCCACAGGAACAAGAAG CCAGTGGCCAGGTCAAAGAAGCAACAATCACCACTGCAAATGAGCCAAACGCCTTGTCCTTGCCCGACGAAATCAGCAAT TCGGTCAGCTCGACGACGATGCTGGCCACGCCCCGCGTAGACATAGCCGCCCAGTATCCCGTCCTGAAACCATTCACAGA GAACGGAGAGCTGGACTGTGCCCGACTGGCCAAGTTCTTCCAGGACACCCAGTTCGAGAGGAAGGAGGAGCAACGCCAGA TTCTGGGCCTCTCCGTAATGGTGCAGTTCATGTCCAAAGAGCTGGATGCGGTTGCCTGCAAGGAGAACAAACATCAATGC GCCCGGACCAAGGGAACGCTAGAGAAGACTATATTGCTGCTACAGCATACGCAAAAGGATTGCGAGCGACTACGCGAGGA TTTGGAGGACAAGGGCCTGGAGTGGATTCAGCGGCAGCAGGAGAAGGAATATCTGCATCGCACTGAACTGAAGCAAGCTG AAGAGAAATTAATGGAGGTGCAATTGCGTGCCAAGTTAAAGTTCTGCGAATTAGAGTCCCAACTGCGTGCCAAGGACGAG GAGAGCAAACAGGCGCAGGAGGCCTACAGAATGGAGGTCTCCCACAAACTTGCACTGAAGCAGGAACATCTGCGCACTGC CGAGCAGAAAATCCAGGAACTGCAGACACGTCTTCAGCAGGTGGAGACCGAGGAGCAGGGGCACCGCGAGGAGCTGATCC GCAAGGAGAACATTCACACCGCCCGGCTGGCCGAGGCCAATCAGCGGGAGCAGGATCTGATCGACCGGGTCAAGAGCCTG ACCAAGGAGCTGAACACCCTGAAGGCCAACAAGGAGCATAACGAACGCGATTTGAGGGATCGGCTGGCGCTGTCCCAGGA CGAAATCTCTGTGCTGCGCACATCCTCACAGCGCCGCAGCCCCTGCACCAGTTTGCCGGACAACGCGTCCGCCGAACTCA ACCGCTTGACCAGCGAGGCAGACAGCTTGCGCTGTGTGCTTGAGCTCAAGCAGGCGGAGATTTCCGCCCTTAGCAAGGCC AAGGCGGATCTGATTCATGAAAGCGAAGAGCGGCTGAAACTGAGTAACCGCGTCGCCCTTTTGGAGGCCCAAAACGAAAT GCTGCGCACCGAACTGGAGGCCAAGACCGAAAAGGAGAAGGAAATCCAGCAAAAGATGGAGGAACTTCAGAAGGCCTACA AATACGAGAGCATTAAACGCACGCGGCTGACGTATGACAAAGAGGAGCTACAGTACCACCTTAAGCAGAGATCATTGCAG CTCCAGTCGGCTGAGTCAAAGTTGCAAGATCTTTCCACTGGTTCCCACGATAACAGCCTATCTAGCCACAGCCGATGCAG CCTGGGTCGCAGTGGCCTGGAGATCGCCGTGACCACCTCCTCGCCCACTTCCCCAGTGATGAAGGGAATGATTGAACGGA ATGATTCCGTCAGCTGGACGCTAGAGATCGAAGATGAGTCGTTCAAGGGTAACACTTCAAAGATTGTGCGCAGGGCGGGG TCACTGCGAAGCAACAACGAGCGGTGCCCCATCCAGCGGCGACAGACATTGACAAGCAATGGACACTCCAATGGATCGGC CACAAATGGCGGCTCTTCCCCCGCTCATCCTAATCCCCTTAGCCAGAGCATGTCGGCCACAGCCCTTCTGAGAAGCAGCA GTAATTCCGGGGAGTCAGAGGGTCGTCCTTTGTCCCGGGCTCGCTCAAAATCGATGTGCATCAAAGCGTCCGCCACCAGT TCGGCGGTGTGCGAATCGTCTGGCCAAAGGAAGAAACCACAAGACGAGTTGAGTCTGGCCGACTGGCCCGAGGATATTCC CCTGTGCTCCAGTTCACCACAAGCGCCGGGAATAGAGATGCGTCCGCGCAGCTCCACCATGAAGCTAATGTCCAGCGAGG CAAAGAAGTTCCAGGAAATCCAGGAGTCCGCCGGCGAGGCCATGGTCTCCGGTGCTAACTCGGAGGACGAAAGCTGTTCG GCCTCATCCGAGGACATAATGCGCAGCTCTTCCGCCTCCAGCACCGCATCGGGTGGATCCCTATCCAAGAGGCCAAAGCA ACCACCATCCCGCATGTCCATCGAGGAGGCTCTGCCCTGCACCCCCATGGAGGTAAGCTGGTCAGAAGACGCTGCCGATG CCAACGGACTGGCATGACATGCTGCCTACGAGGAGCACGTCGACCAGATTGCCGAGGCGGCTGAAGAGGCTTACGAGAGC GAGGACATTGAGGAGGATCACGATGAGCTGATCGTGGGCGAGGAGCAGCCGGAGCACAGCGACAGCGATGATAGCTTCTA AAGCGCTAAAACCAATTTGTAAAACAAAATAATGAGGCAGTTAGAGTCGGCACTTGCGGCGAAGGTGGAAGTCATAGTCG GAAGCATAGCTTAGCGTTTTCACTTCTATAGACCCCGTACATAAACTATTAAGATACACAAAAATATATAATGCTAATTC GTACGTTCTTAACTGAAATGATTGTGTAAATAATTGCATTTAGCAGTATGGTAGCTCATAGAAGGCGCTCATGAAATATA ATAATAATCTAACAATTAGTCGAGCACAGCAAGCGGAGCCGTCACAGCAACTGGGGGGGAATTGAAAAACATAAATGAGG GAAGTACTTTTTTAAACGGGCGCGGACGAGGATCGAAGCCACACACAAAGCTAAACCAAGTTTAGAGCGGTATAGCTGCG TATTTAGAACAGTTTCAAACTACACAGAAATCAGTAATGGGGGTAGCATTTAGATGAAGCAGTATGAATATTTGCATAAA ACCAACCAACTTATTACTATACCACACACTATAAGTATTTGTATGACTAGCGATATTGATATTGTATTCTAACCGCAACT CGCAATCTCCGATGTCGAACATGTTACTCGATCCAATCCAGATGCTAACCTCTCTTGAGCTCAAATATAGCACTCACTTG ATGTCCTTAATTGAAAACCTAATGCCCGCTATTTGTGGACCAGGTGAGGTATCCGCCCCCTGCCACGCCTATATTATAAT GTACCAATTAGAGTGTCAGAGGTCTGAAGCTGCATCGCCGACCGAGCTAAGCTTTAATTTGTGTTTTATATATGTATGTA TTTATTATTTAGTCTTTAATGCCTTATGAAAGCGTTAAATAAATGCAAGAAATTGAAATCACTTAGTTCGTATGGAATGA GTTATATATTAGTTCTAGCCTAACTACATTAGTTTATGTTACGTTTTTATTTTGTTATCATTGAATTGTGTCCGAAATAT GCGAGTTTTATATGTATAGGTTCAGGATACGCTAATTTTGTAACATGCAGCATTGTCATTAACTTTTAAGTATGTTATTT TTATTATGTGTGAGATATTAAGAGCTCCCGGCACCCAACAAAATGAATATTATGTAAGAGACGGTGCGGAAACTGTCAGA AAAAAAGTTGCAAGAGAAAATAAGTAAAATGGTACAGTTAAAAAATCACAATAAATATTTTTTGCTAAATAATTTCAAAA CCCTTAAATAAATAAGTAATTGTTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Cloned cDNAs prepared from polyadenylated RNA isolated from 0-22-hour embryos.
Embryos at 0-22 hr AEL were collected from an isogenic y; cn bw sp (iso-1) strain.
RNA was poly(A)+ selected twice. cDNA made using Stratagene ZAP-cDNA synthesis kit; oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. For clones LD21101 and higher, cDNA directionally cloned into EcoRI/XhoI-digested pOT2 plasmid.
