FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Clone: Dmel\LP04393
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General Information
Symbol
Dmel\LP04393
Species
D. melanogaster
Name
LP04393
FlyBase ID
FBcl0179765
Feature type
cDNA_clone
Computed gene(s)
Dmel\mbl
 
Collection Status
BDGP
Known Problems
contains transposon sequences
FlyBase assessment
    Library
    LP_cDNA
    • LP cDNA library of D. melanogaster, iso-1 strain, larva and pupa.
    Strain
      Vector
      Tissue Source
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      larval stage
      organism
      (Rubin et al., 2000)
      pupal stage
      organism

      Comment: early pupae

      (Rubin et al., 2000)
      Sequence Data of the Insert
      5' Sequence
      Total bases
      691
      NCBI
      AI260504
      5' sequence dataSequence Downloader
      CCAAGATTCCTTCTTTTCTTCCTTGTACGTTTTTGCTGTGAGTAGGTCGTGGTGCTGGTGTTGCAGTTGAAATAACTTAA
AATATAAATCATAAAACTCAAACATAAACTTGACTATTTATTTATTTATTAAGAAAGGAAATATAAATTATAAATTACAA
CAGGTTATGGGCCCAGTCCATGCCTAATAAACAATTAAATTGTGAATTAAAGATTGTGAAAATAAATTGTGAAATAGCAT
TTTTTCACATTCTTGTGAAATAGCTTTTTTTTTCACATTCTTGTGAAATTATTTCCTTCTCAGAATTTGAGTGAAAAATG
GACAAGGCTAAACGTAATATTAAGCCGTTTGATGGCGAGAAGTACGCGATTTGGAAATTTAGAATTAGGGCTCTTTTAGC
CGAGCAAGATGTGCTTAAAGTAGTTGATTGTTTAATGCCTAACGAGGTAGATGACTCCTGGAAAAAGGCAGAGCGTTGTG
CAAAAAGTACAATAATAGAGTACCTAAGCGACTCGTTTTTAAATTTCGCAACAAGCGACATTACCGCGCGTCAGATTCTT
GAGAATTTGGACGCCGTTTATGAACGAAAAAGTTTGGCGTCGCAACTGGCGCTGCGAAAACGTTTGCTTTCTCTGAAGCT
ATCGAGTGAGATGTCACTATTAAGCCATTTTCATATTTTTGACCAACTTAT
      Library Information (1)
      Library: LP_cDNA
      Description

      Cloned cDNAs prepared from polyadenylated RNA isolated from larvae and early pupae.

      (Rubin et al., 2000)
      Sample preparation

      Larvae and early pupae at various stages of development were collected from an isogenic y; cn bw sp (iso-1) strain.

      Protocol

      RNA was poly(A)+ selected once. Oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. cDNA directionally cloned into EcoRI/XhoI-digested pOT2 plasmid. ~500,000 colonies were plated out and a mass plasmid DNA prep was done using Qiagen Plasmids containing the cDNA inserts were size selected on Sephacryl S-500 column in order to exclude plasmids with no insert. Early elutions were collected and transformed into DH5 alpha strain (Gibco/BRL).

      (Rubin et al., 2000)
      Comment
      Reported As
      Symbol Synonyms
      LP
      (Rubin et al., 2000)
      LP_cDNA
      References (1)
      Review (1)
      Rubin et al., 2000, Science 287(5461): 2222--2224
      A Drosophila complementary DNA resource. [FBrf0127297]
      External Crossreferences and Linkouts ( 1 )
      Linkouts
      DGRC - Stock center for Drosophila cDNAs, vectors, and cell lines
      Synonyms and Secondary IDs (0)
      Reported As
      Symbol Synonym
      Secondary FlyBase IDs
        References (0)