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FB2026_03
,
released September 17, 2026
Comment: early pupae
TTGAATCGTTCGCATCGGGTGCTCGCGTTTGAATGTTGTACGAGTATCTCAACCCTCTCTTTCTCTCGCTGGACTGAAAA CCAAGTGAAACGATCGCACAAAACCCAATTAAAGTTTATAATTAATCGGCGAAAAGGTCACGCTGGTCGAGAGTTGAAAG TGCCACCAGCAAATTCGCTAATCTAGTGGATCGATCTGAAAACTCCAATCCATCTCGCCCCGTGTGGCTGTCTGTATACT GTGTTCGAGTGTACCTTCGAGGTATCGAGAGGCATCGAGGTATCTAAGCGCACATCTACACTGGGTCTGGCTGCCTGTTG GACCTGGAGTTAAAGTTGGAAACTCGTTTTTCAAAACCAAAACGACGAAAAAAAAAAAGTCTGCACAAAACGAGAAAATA AGCGCGGCCGCGAAGAAAACAACAGCAGCAACTAAAGTTAAAAGTGGGGGGGAAAAAATAAAGCACAATAATATAGGAAA AGCAAAAGCAGATCCAGAACCAAAACCAAAACCTGACGGCCAGGCTTCCAGTGATTTGGTTCGGTTTGCTTTTTGGCCGT CGCTCGCCCTTCTGCATTCTTTTCGCTTGTTTACTTATTTTCGTGTGCTGTGCGGTTGCTTCACTTGTTGCAGCTGCACA AACGTTCGCCCAAACATATACGAGTATATCCCGCTCGCAACATACGTATGTATATATATTTATTCGGACTCGGTTTCGGG TTCGGTCTGTATGGCATAGATATAGATACGGTGCCTGCCAGAGCCTGTGCGGAAAACGAAAAACGAAATACGAATACGAA TACGAAAACCTGTTCCTCCGGCCATTTGCGAGTGTATCGGTGGCGGTGAGAGCCTGTATACGGAATTATTCAAATAAGAA AATCGCCGTCTTGGTCGTCGTAGTCGTGGCCACACAAATGTTCAACAACGATGGCCACATTGGGCCTGTCAAAAGTCTTC ATACTGGATAAATACTTTACCGAATTGCAAAAATTTTGGGAGACGGAGAAGAAGCTGCAGGATGCATCTTCATCCAACGA GGCAGTTCATCTGCAGCAGCGGCTCAAAAGCCTGAGCACCGAATTGGTGACTCTGCGAAACCGCCTGCATGTGGGTCATG GACCGGGTCAGGGTCAGAGCCAGGGAAATGGCGCGCAGCCCGTCGCCCCAGCTCCCAATGCGGGTCCCAAGGCCAACAAC TTTGACCTAAACGCATCCAGTCCGAATCTCAATCTGAGTTCCAGCGGCGCAGGACTTTCAGCCAGTGCAGTTCAGCAACA CACCAATGGACATCACACGACATCGAAGAATCACAGCTTCAGCCACACATTGCCCGCAAACTCGGGCTCTGGTGGAGGAG GAGGCGCTGGAGGAGGCGCCGTTGTGTCGGCGAGGAACACGTCGATTCCACATCCACTTCCGCATCAGTTGGCCGAGAAG CCGGGCCTGTCGCATCAGCAGAGTGGCAGCGGACATGGTCAGTCCACCGGGACACTGCCACATATGAGCGGCATGGGAAG TATCCTGGGGCAAAATTCCCATTCCCATGCACCGGTCAACAACAACAGCAACAACTCCAACACGCTGCCCATGCGCACCT CCAACTCCGGACACCTGGGCATCAATGGCGGAGGCGTTGGCCACCATCTCAGCCACGCCCACAGCCAGCAATTGCCATTC ATCCCGCAGTCGAAGCACACGAATCCCTGCCAGAGTGTGAAGACTCTGCCCTTTGGATTCGGATTCTCCGAAAGTCAGCA GAAGATGCAACAGCAGCAGCAACAGGGCAACTCCTCGCCCAAGGACATGCAGGATCTGATCCACTTGTCGGGACCGCTCA CCGAACATGCCGTGATGCGAACGCTCCAGGCCCGATTCAACGAGCGGCGGTACTTTACGAACGTGGGTCCAATCTTGCTC TCCATCAATCCGTACCTGGACGTGGGCAACCCGCTGACCCTCACCTCCACCCGCGCCATGCCGCTGGCCCCGCAGCTGCA GAAGATCGTCCAGGAGGCAGTGCGTCAGCAGAGCGAGACGGGCTATCCGCAGGCCATCATTCTCTCGGGAACATCGGGCG CTGGAAAGACGGCCAATGCGATGCTGATGCTGCGCCAGCTCTTCGCCATCGCCGGCGGAGGTCCAGAGACTGATGCCTTC AAGCACCTGGCAGCTGCATTCACAGTACTTCGATCCCTGGGATCCGCCAAAACTACGACCAACTCGGAGTCCAGTCGCAT TGGCCAGTTCATCGAGGTGCAGGTCACGGATGGGGCACTCTACCGCACCAAGATACACTGCTACTTCCTGGACCAGACTC GCGTCATTCGTCCACTGCCGAAGGAGAAGAACTATCACATCTTCTATCAATTACTGGCTGGTCTCAGCCGGGAGGAGCGC CAGAAGCTACACCTGGATGGCTACTCGCCGGCCAATCTGCGCTACCTGCGAGGTGACATTGGCCAGAATGAGCAGGAGGA TGCGGCGCGCTTTCAGGCATGGAAGACCTGCCTCGGCATCCTGGGCATCCCATTCCTGGACGTCGTTCGTGTCCTGGCCG CCGTTTTGCTGCTGGGAAATGTTCAGTTTATTGATGGAGGGGGTCTCGAGGTGGATGTGAAGGGTGAAACGGAGCTGAAC TCTGTGGCCAGTCTACTTGGCGTTCCGCCGGCGGCCCTCTTCCGTGGATTGACCACCCGTACCCACAACGTTCGTGGCCA GCTGGTGAAGTCCGTGTGCGGCGATGGCGATGCCAATATGACGCGGGACTGCCTGGCAAAGGCGCTCTACTGCCGCACGG TGGCCACTATTGTGCGGCGGGCCAACAGCCTGAAGCGCCTGGGATCCACGTTGGGCACCCTCAGCTCGGACTCAAACGAA TCGGTGCACAACCAGGCGGATGTCGCCTCTCAGCACGCCTCCACCATTGGCGGTGGCAATGCGGGCTCCAAGTCGATGGC GGCACTCAATAATGCAGTGCGCCATGCCACCTCCGATGGCTTCATTGGAATCCTGGACATGTTCGGTTTCGAGGAGCCCT CGCCACATGCCCATCTGGAGCACTTGTGCATCAACCTGTGCGCCGAGACCATGCAGCACTTCTACAACACGCACATCTTC AAGTCCTCCGTGGAATCCTGTCGCGACGAGGGCATCGTTTGCGACACCGAGGTGGACTATGTGGACAATGTGCCCTGCAT CGATCTGATATCCTCGCTGCGAACCGGTCTGCTCAGCATGCTGGATGCAGAGTGTTCGGTTAGGGGCACTGCCGAGAGTT ATGTGACCAAGTTGAAGGTGCAGCATCGCAGCTCCACTCGCTTGGAGACGAAACCCACAGCCGAACCACACGATCCACGC ATGTTTCTCATCCGTCACTTTGCCGGTCGGGTGGAGTACGATACCACGGACTTTCTGGACACCAACCGTGATGTGGTGCC CGACGATCTGGTGGGTGTCTTCTACAAGCACACCTGCAACTTTGGCTTCGCCACCCATCTCTTTGGATCCGAGCTGAAAG CCCTGTATGCTCAGCAACAGGCTCCAAGGGGTCTGAGCTTCCGGATCTCGCCCACATCACACTCGGATCTGCTGAACGGG GACGAGCCGGTGTCCACGCTGACCCAAGACTTCCACACCAGGCTAGACAATTTGCTGAGAACCCTGGTGCACGCCAGGCC GCACTTTGTGCGCTGCATCCGAAGCAATGGAACCGAGGCGGCTCGCAGCTTTGATAGGGCTACGGTGGTGCGCCAGATTA GATCCCTGCAGGTCCTGGAGACGGTCAATCTAATGGCCAGTGGGTTCCCGCATCGCATGCGCTTTAAGCAGTTCAATGCC CGCTACCGTATGCTAGCCCCATTCCGCCTCTTGCGCCGCAGCGAGGACAAGGCTCTGGAGGATTGCCAGTTGATTCTGAA GTACGCAATGGAGCAGCCGCCAGTGCTGGATGGCTCGGTGACCCTGGCCTGGGCACCTGGCAAGCGTCATGTCTTCCTTA GCGAGGGCATTCGCCAGCACTTGGAGCACTTGCGCACCGAGATCAGGCACAAGAGCGCCACCCTGATGCAGGCCACCTGG CGAGGCTGGTGGTGGCGCAAGAAGATGGGCAATGGCGGGGCCAAGCGCTCCAAAATCCCAGGTCTTCAACAGGCGCCACT GCCGAACAACAAGACCACGCCGAACTCCGCCGCACAGAACAAGGCCGCATCCTCCACAATGGCCGCCCTTGCCGCTGTGG CAGCTGCTGCTCCCATTTCTGTACCACGCCTCTCGGCCAAGACAACGAGCCTGGGCATTGGAGGCACTGTGGCCAGACCA AGGCCACAGCCCATTGCTGGGACTCCTCCGCCGGATCCGCAGGAGAAGTGCGACCAGAAGATCATTCAGCAGACCTGTAA CCTGTTTGGACTCGATTTGGAACGTCCGCCGCCAGTTCCGCCGTCTCGTTCCTACACGATCACCGGGAATTCGAAAATAA GCTATCCCCAAAACCGGGTCATGAAGATGAACTTTCCAGAGGAGGGACAGTCGGAGGCGCCGCAGCTGAAGAAGGGCGAA GCGGTGACCGTAGTGGGGGCCTCCACCGTTCGGGGTCACCTGATGGTGGAGCACAAGGGGCAGAGCTTCCACGTTCCCTT CCAGTTCATGACGCTCAGTAAATAAGGACTACTGGTGGAAACAAACTGGTTATGATTCCATTCATTCCATCTGTTCAACT CTTCCTGATTTTAGACATCCCATTTCTAAAAGACTGTAAATATTCCATTTGGTAGATTAAATAACAAGCATTCTGTCTAC GAAGCGCATGCTAACTGAGGTGCTCTAATTGGCCCCTAACTTACGTAGTATTATTATTGTCGCGTTAATAAATAAACGCC AATCGATAGGTTGTAGCTTCAATTACATACCCACAAGTGCCAATCAAATAAGTTCTTCCACCTAATTCATAAGTATTTTA CATGTTACTGAGATTACATTATTCGCCGTTAAGTAATTAGTTAAGTAAGTTTCAACAACGACATTCATTTTTTATTTTTG AGTTCACTTAAGAATTGTGAATTTTATTAGACTAAGGTTCATTTTGTCATTCAAATAAACGAAGCATGCATTGAAATATT TAAAAAAAAAAAAAAAAAAAAAAAA
Cloned cDNAs prepared from polyadenylated RNA isolated from larvae and early pupae.
Larvae and early pupae at various stages of development were collected from an isogenic y; cn bw sp (iso-1) strain.
RNA was poly(A)+ selected once. Oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. cDNA directionally cloned into EcoRI/XhoI-digested pOT2 plasmid. ~500,000 colonies were plated out and a mass plasmid DNA prep was done using Qiagen Plasmids containing the cDNA inserts were size selected on Sephacryl S-500 column in order to exclude plasmids with no insert. Early elutions were collected and transformed into DH5 alpha strain (Gibco/BRL).