ATTAACTTGGGCAAAATGCAGCGAGCACGACTGTTGCGGAACGTGTGGCTCTGCAGGCAGCGCTTGGGCCATCTGCCAGG ACGCAGCGCTTCCTCCGCCAGCCTGGCATTCCGTTATGCAACGAAGCCTGCGGCGCCAGCGGTGCCGTTCTCGCTGCTCC AGATGCGATCCCTGGGAACGGGTGGCGTCCTCAGTGCGGTGGAGCGAAGAAGCCGGCGCCCCGCCTCCTCCTCTGAAAAA CCGAAAGCCAGCGTGGCGGATGCGTGGACACAGCTGGAGGCGTACTACCGGCAGCACGATCTGATCAACTTTGAGCACTG CCAGCGCCTGCTGGAGCTCCTGCAGGAGTCTGCGTCGCCGCTAGGAGTCGGCATCGAAGAGGTGCACTTCCTTCTAGGGT CCTGTGTGCCCGAACTGCTTCCTGCTCAGAGCAGCCAGGAGCGTCTTCAGCTCTTCAAGGGACTCTGGGCTCAACTGATC AAGTTGGGCCAGCCCACGCTGGCACATTACCACACGAAGCTCCAGGTGCTGCGCCAGAACCGTTTGCCTCTGACTGACTA TCGCTCGTTGCTGGCGGAGATTGCCGTCTACCATGGAGCAGCCGATTCCACCTTGTACGGCGCTCTTTTGGACGTGGCCG GCGCCGCCGGAAACATGCGCATGGCCACTGAGATTTTGTCGGAGATGCGCGAGCGCAGCCTCGCCCTCACGGAGCACAAC TTTCACGCCCTGCTTTTGGGTCACGCCCGCAGCGGTGATCTGCCCGGGGCGGAGTCCGTCCTAGCCAGCATGCGGGCCGC AGGAATACAGCAAGGCAGCAGCACTCAGGCCATTTGGTTCGAGGCTCTGGTGGAAAACGGTCAGTTGGCACAGGCCAAGG AGCTGCTGCAGGTGGAGCACGGATTCACCGCCCCTCAGCTGCTCCAGATGCTGCGGGGTCTGCTCGCCAGCAAGGATGTC GACACGGATCTAATCCAACTGCTTGTCAAGGAACTGCCCCGCGAGTTTCTAACAGGTATCGACCTGCCGCCCGCCATCAG AAGCCTGTGCATTCAGCTGGTGCACCAAGGACGCACCGAAGTCGTCATACAGCTCGTAGGTGCGTTGCCCGCTCCGAAGT TCGAGTTGCAGAATCAGAACGTGGATGAGTACGGTGCACTTCTGCTGCAGGAGTTCTTCCGCGCCCAAGTTCCGTTGGAC CAAACAATCCAGTTCGCCGGTCAACTAGTGCAGCGGAACCAGAACGTCCGCGCCCTGCATTTGGTGGCGGAGTTGGCCCT TCGCCGTCAGCCATCATCCGCGCTACGCTGCCTGGAGGATCTATCTGCAGCCGGCGAGGAACTTCGTCCGCATTACTTCT GGCCCCTGATCTTGCACCATTACCGGCGGGAGGGCGAAAGCGGCATGCTACGCCTGGTTTCTGAAATGCGGCGCCTGCGC GTTGAGTGCGACGAGGAAACGTTGCGGCACTACCTGTTTCCCCACCTGCGCCAAACCCTGGTGCAGCCCTTGGAGGCCGT GAAGGCACTTGAATCGGCGGGGGTCAAGCCTTCCCAGACCCTGTGCCCGGTTGTGAGCCACTTGCTGCAGCAGCATCAGA TGTCCGAGGTGCTGGACTTGCTTGATCGGTATCCGACACGCTTGGACCTGGCCACGCTGCTCCAACCACTTTCTTCGCTG GCGGTCAATGCCCGTGCCACGAAGCGTTTCGAGGTATTTGCCAAAGTGATCCAAGCCCTGCAGCAGAAGGCACTCAAACG GGGAGAAGATTTTGTGGGCTCCTTGCTGCTGCAAATGACTGGACCTCAGACGCGTCTGCGCCAGGATGCCACCTCGTTGC GACGTTTTCTGCACGAACTGAGACGCCTAGAGCTACAAATCTCACCCGCTGCCTCCGAAGCTCTACTCTCCGTGGTCCGG CACGCCACTGATGACGTTGACCTCATCCAGGATCTGGCCAAAACGCTGCAGAAGATGAAAAACCCACAGCTCGCCCTGCC AGCTGACACGGTCTCGCTCCACGGAGGCTTCATCAAGCACCCGCGCGATATGAGCATAGACGAGCTCGAGTGCCACCTAG TCGAGCTGGAGTCCAAGCAGCTGAACCCGCGAGGCGTGTTGCGTCGCTTGCTGCAGCTCTCGGTGCGCAGTGGGCGCTTG GAGCGCGCACAGCAGCTGTTGGCCAAGTGCCAAGTTCTGAAGGTTCAGGAAAGCGCAGGCATGATGGCCTCCATATTCGA CCTACACATCAAGCTGCGCGACTTGCCGCGCGCCCAGGAGAGCTTGGAGCGACTGAGCAGCATGTATCCAGCCTTTCAGA TTGACGAGCACAAAGTGATCGACTATGCCGCCCTCCTGGTCCAAGGCCAGCAGCTGGAGGCCGCAAAGCAGCTGCTCCAG CGACGCGCGGAGCAGCATAAGGTTGTCGGTGGCGACTATGTGATGAAGAACGTATGGCAATTGCTGACCAACGTGGGCCA GCTGGCCGCCTCTTCACATGCAGGTGGCGAGAGCTCCAAACAACTTAACCAGACTCGCGAGTGGTTGGACTTCCTCAGGG GTCTGGGCTACTGTCAATCGCAAAATGCCCTACTGGGACCAGTGGTCCGCGAGTGGCTGTTGAAGGGCAACTTGGACGCT GCGGTGGCCGAGTTTCAGCGACTGGCTAACACTCACAAGCACACGCCTCTGCAGTTCGAGCTCTTGTCCCTGCTGGTGCG ACTGGGCAACGGGGACGAGCAGGAGCTGACCCGTTTTCCCGGCGTCACGGCCGAGTCCGCGCAGCAGCACCTAGCGGCGG TTACTGCGACTGTGAGTCGCATCCACGGAGCGGCCAACATGAACAGCGCTCTGCTGCTCGCCCTGGCCGAATCCGGAAAG GAGACACAGCTGCGCCGGCTGATCATCAACCCAGAGTTCCGCATCAACCACGAGCTCCTCGTGAAGAATTGCGAGCACTT GGGACAGGAGGGCGCTGTGCGGACGCTCCTGCGGCTGGCGCGCGGAGTGAGGGGTGTCCAGCGGACCATAGACGAGCAGA AGATCTACGACCTCCTGTTGGCCCAGTTCTGCCGTAGCAACGACTACGAGGCGGCGTTGGATCTCTTTGAGCGCTTGGAG GCCGACGACGAGCTGAAGGTGTCGCATGAGTTCCTTCGCAACCTTGTGCAGCTGCTGCAGGTGAACCAGGTTGAAATTCC CAGTACAATCGCCCTGCGCGCCCACGTTAGATAGCCGGGCGACGAGATAGATAGATCGTCGCTCTTATCCTTAGTTATAC ACTTGGTATGTATGTACACACATAAATTAGTGCAGTAAATACGAGACGGTATACCTGCAAAAAAAAAAAAAAAAAA
Cloned cDNAs prepared from polyadenylated RNA isolated from adult male testis. The strain used was not the iso-1 sequenced strain.
As a source for stem cell-enriched tissue, testis were dissected from adult males 0-3 days old that overexpressed upd (P{UAS-os.C}) in germ cells from the nos-VP16 GAL4 driver (P{GAL4::VP16-nos.UTR}).
RNA isolated from dissected testis overexpressing upd to enrich for stem cells was kindly provided by M. Fuller. cDNA directionally cloned directly into EcoRI/XhoI-digested pOTB7 plasmid. cDNAs were transformed into DH5-alpha TonA.