FB2026_03 , released September 17, 2026
Clone: Dmel\AT25231
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General Information
Symbol
Dmel\AT25231
Species
D. melanogaster
Name
AT25231
FlyBase ID
FBcl0296660
Feature type
cDNA_clone
Computed gene(s)
Dmel\Strip
 
Collection Status
DGC gold BDGP
Known Problems
none
FlyBase assessment
    Library
    AT_cDNA
    • AT cDNA library of D. melanogaster, Oregon-R strain, adult male, testis and seminal vesicle.
    Strain
      Vector
      Tissue Source
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      adult stage | male
      testis
      (Stapleton et al., 2002)
      seminal vesicle
      (Stapleton et al., 2002)
      Sequence Data of the Insert
      Full Length Sequence
      Total bases
      3,167
      NCBI
      BT003798
      Full Length sequence dataSequence Downloader
      ATATGGCTTGGAAACAGAAAAATAAACTCCTTTTTGACACTTGTAAAAACCAGTTAAGCTGTGGCGCCGTAAACGCCAGA
TCCTCGATGATGCAGTCCATCCAGTCTAGGATCAACGTGCTTAACACCATTGCTGCTTCCAGCAAGCGGATTGAACGCGC
CAGCGTTGACACGGACACCCACGATGCGGACGCCAACTGCGACGGGCCCGACCTGGACTTCGTGTACGCGGACGTGGATG
GCTATCAGAACGAGATCGCTGAACTGTATAGCTACACGGAGTTCAGCGAGTTTCAGATGAACGTGAAGGCCTTCGAGGAC
CAGATGGAGCTGTACGACCTGCCGCCCAACTGGCAGAAGCAGGACTCCGCCTCCCAGCGCGGCATCGTGATGAAGCTGGT
GGACCAGCTGGAGGTCTCCGATCGATCCTTTCGCATGCAGGCCGCCCGGTGCATCCTTTACCTGGCCCAAGGCTGCTGGG
CGGAGGTTCAGTCGGACGAGGAGCAGCACCAGATCACCAGGGACAATGTGCTGGTGCTCTACCAACTCGGCGTCTTCGCC
TCCTTCATCGACCTGCTCAACATGGAGATCGAAAGCGCCTGCTCTCCGGACATTGTGGCCGTCAAGATCACTAACGTCAC
GCTTGTGGACTCCACGGACATTCGGGTGATCCTATCTGTGCTCTACATCATCACAGAAACGATTCGCGATGAAAAGGAAA
AGGGCAGCGAAGACTACAGAGAGCTGGCCGACTCCTTTGTGCAAGAAATCAACTCACCACTACCAGATGGCGAACTTCTG
GCCGTCAAGCTGTTGGGCATGATCACACGGTTTTGCAGTGGCGCAGCACCGCAGTTCCCCATGAAGAAGGTTGTCCTCCT
GCTGTGGAAAGTTTCGCTGCTGGGACTGGGAGGCATGGATGTGCTCAAGAATCTCAAGAACGAGTATCGATTGAAGGTGG
GTTTGGAACCCATCACCGAGGACACGCTGGAGGTTACCAAGTGCATGCGAGCCAGCTCACCGCCGGCCACAGCTACTGAT
ATACTGGAGAACCAGTATCCCAAGCGGAACTTCAAACGCTCCCTGATGAAGCAGCGTTTTTTGGACGAGCCCGAGCAGAT
AGACATGGAGATGGGCGGCAACGAGGGTCAAAATAATGCCAACAATGGCAGCGGCAATGGCGGAAACGGGGAGGAGGAAC
TCTCTCAGTACTACAGACCCTACGATGACCCCTCTTCGTCCGCATCCACCACCTCGACGGCAAATCCAAACAACCAGCCT
AGCCTAACACAGCCACCCGCTGCTGTGCCTCCGGTACAGATCACGGCTCGCCTGCCCTGGACGCCCAAGGTGCGACAAAA
GGACATTGACCAGTTTCTGGACATTTCGCGGAATAAGTTCATCGGGTATTCGCTGATTGGTGACCACGAGAGTCTGGCTG
GACTGCCGCAACCCATTCACGAGGGCTTTAAGACGCTGCAGCGGCACATGTATATGAGCCTGGCCGATGTGCAAATCAAG
AAGGAGGAGGACATAGCTAGGAACCCCATTTCCACACACGAAGACGAAATCAAACTGACCCCGGCTGAGGTGTTGTACCA
AGCCATCCTGCCAAACCTGCCGCAGTATATGATCGCCCTCCTTAAGGTCCTGCTGGCAGCTTCGCCAACGTCCAAGTCCA
AGACGGAGAGCATTAACATAATGGCCGATGTGCTGCCTAGGAAGATGCCGCTGACGGCCACCCAATCAACAAAGCTAACC
GTCGACATTGGTCGGCACAAGGAGATCATTGTGAAGGCTGTCTCGGCCATCATCCTGCTATATTTAAAGCATTTCAAGAT
AAACCATGTTTACCAGTTTGAGTTCATGTCGCAGCATCTCGTGTTCGCCAACTGCATCCCGCTGGTTTTGAAGTTCTTCA
ATCAGACAATCACGGAGTACGTGAACACCAAGAACTCCATTCCTCTGCTCGATTTCCCGTCGTGTGTGATTGGTGAGCAA
CCGGATTTGAGTGGCGATAGCTTTGTGTATGGCGGAGAGATGTCCGATAAGTCCTACTCTTGGCGGAACGTGTTCTCCTG
CATAAACCTGCTGCGCATCCTCAACAAGCTGATCAAGTGGAAGCACTCGCGAGTGATGATGCTGGTGGTATTCAAGTCGG
CACCAATCCTGAAGAAGACGCTTAAGGTGCGCCACGCCATGATGCAGCTGTACGTGCTGAAGCTGCTCAAGATGCAGACC
AAGTACTTGGGCCGCCAGTGGCGGAAGTCCAATATGAAGACCATGAGTGCAATTTACGCCAAGGTGCGGCATCGTCTAAA
CGACGATTGGGCCTTTGGCAACGACTTGGAGTCGCGTCCCTGGGACTTCCAGGCGGAGGAGTGTACGCTGCGGGCCTGCG
TGGATCGATTCAACCTGCGACGCTATCCGGAAGCCACCCAGAAGTGTGGAAACAACGGCACGGCAGCTCAGAATGCGGAG
AACGCCCAGCAATCCAACATGATGGCGGGCAACAATGGATGTGGCAATAATGGCGACGCAAATGGCTATGGAAACAACGG
TGGCGGTGGCAATGGCGGCCACAACCAGCAGCAGCAGCTCAACGACTATGGTGTGGAGGGTGGCTTTCCCAGCGACGAGA
TCCTTACCCACTCGGACTTTGCCTTCTTCGGTCACTCGGGTTGGTGGGATCGCAAGGTCGAGCTGACGGATTACTTCAAG
GCCAATTACGCGGTCTGGCTGGAGGAGGAGGTCTACAACAGTCAGACCGACTGGGACGCCCTCTAGGGCAGGGCTGCCAT
CGCTGCTCTAATATCCACATGCGCGTCATAAAAGCAAGAAGTTATTAACGTAATTGTAAGCATTAAGAAGTACATTGATA
TAATCCATGTAGCCCGACCACGATTGAAACACCAACTACTCACTGAATTGGCAATAATTTGGCAAGCCACGGGCCTCCGC
TGCGCTCCTCTTCACTAACTAGCATACGCGAATACCATACCCATAGCGATGACAAGCCTGTATATGTAATGATTGTATTT
TTTAATTTATATATTTATTATTTTACCTGTTCTGCGAGCCCTTGAAACGGATCGCGGCAATCGGCTTCTGTAGCGGAATA
AACAAATTTGCTAGTTCGAAAACGAAAACAAAAAAAAAAAAAAAAAA
      Library Information (1)
      Library: AT_cDNA
      Description

      Cloned cDNAs prepared from polyadenylated RNA isolated from adult male testes and seminal vesicles.

      (Stapleton et al., 2002)
      Sample preparation

      Adult male testes and seminal vesicles were hand dissected from 0-3 day old non-isogenic Oregon-R males from a population cage.

      Protocol

      RNA was extracted from dissected testis and poly(A)+ selected twice (kindly provided by J. Pringle and M. Fuller). cDNA made using Stratagene ZAP-cDNA synthesis kit; oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; cDNA size fractionated on Sephacryl S-500--approximately 1-6kb. cDNA directionally cloned directly into EcoRI/XhoI-digested pOTB7 plasmid. cDNAs were transformed into DH5-alpha strain (Gibco/BRL), or for AT121nn clones, into DH5-alpha TonA.

      (Stapleton et al., 2002)
      Comment
      Reported As
      Symbol Synonyms
      AT
      (Stapleton et al., 2002)
      AT_cDNA
      References (1)
      Research paper (1)
      Stapleton et al., 2002, Genome Res. 12(8): 1294--1300
      The Drosophila Gene Collection: identification of putative full-length cDNAs for 70% of D. melanogaster genes. [FBrf0152058]
      External Crossreferences and Linkouts ( 1 )
      Linkouts
      DGRC - Stock center for Drosophila cDNAs, vectors, and cell lines
      Synonyms and Secondary IDs (0)
      Reported As
      Symbol Synonym
      Secondary FlyBase IDs
        References (0)