TTTTCGATCGCGGATGTGTGCTTTTCGATCTCGCGATCTCTAATTTCTTTAGCAATCTATATATATGTACATATTGCACG GTGGCAGCAGAGAAAGAGAATAACAACTGCATATCATATGCGATATAGCAAGCGTTTGTCTTAGTGTGCGTGGGTGTGCA AATAACAAATAATACGACATGAACGACCAAGAGAAACAACACCAGCAACAACAACAACACTACTTCCACAGCGAGTGGAA GACTACCGCTAATAGTCATGGAAATTCGTTATCAGACAGCCGGGATTATGATACCAACTCGTCGCGCAATTCGCTTTCCG GCTCCCCGCCCGTCACATCCAATTGCTCCTCCTCTGGTTCCCTGGACTTCGACAGGCCACTAGCTCAGTTGCTCGAACCG AAATTGGAGGCCGACGGGCTGGGCATCATCCATGGGGACAACTACAGCATCTACGGCAGCCAGACGGCGGAATCCACAAG TGGCGAGCAACAGGTGCTTAATCTGGGCCTCACCCTTGATACGGGGGACGCCCAGGCGACATATGGCGACCTATTCGGTG CCCAGGATCAACTCACTGCATCTCAAACTCATGGCCATTCACAAGTTGTTGTATCTGCGGCAGCGGACAACGCTTTTTCG CTTGCGGATCAACTAACACCATTGCCCATCACGGTCATACCCATCACATATCACCATACCACCGAGAATCTCAGCTCCAG CCACTCTGAAGTTCCGCTGATAGCCAGTCTGAGCGATATCACCGCCCAGGTGTTCAATCTGGATGACATCTGCTTCACGC TGGAATACCAGTTCGAGAACCAGAGGCTCGTCCAAGTCCAGCCGCCCACTGTGGTCTCGCTCAGCATGGTGTCCAGCCCC AACGAGGCTGCAAATCCTCCAATCAACTGTGATAACGACCAGGGAACTGGAAACAGCATCTCTAGGGACTCCACATCCAA TTCTCCAGCTTATTTCACAATTGAGACGAGAGTTCGGATGAGGCTCTCAGTCGCTATAGATGCAATTATGAGAACTGTTA TCGCAGCTACAGCACAATTGGAAATCTGCGAACGCATTTAAAAACCCACACGGGCGACTACAGCTTTAAATGCCCGGAAG ATGGATGCCACAAGGCATTCCTCACTTCCTACAGCCTGAAGATCCACGTCCGCGTCCACACGAAGGTGAAGCCATACGAA TGTGAGGTGTCCGGCTGCGATAAGGCATTCAACACGCGCTACAGATTGCACGCCCACCTTCGTCTGCACAATGGCGAGAC GTTCAACTGTGAGCTGTGCCAGAAGTGCTTCACCACGCTGAGCGACCTGAAGAAGCATATGCGCACGCACACCCAGGAGC GGCCCTACAAATGTCCGGAAGATGACTGCGGCAAAGCCTTCACCGCCTCGCATCACCTGAAGACCCACCGAAGGACACAT ACAGGCGAAAAGCCGTATCCCTGCCAGGAGGACAGCTGCCAAAAGTCGTTTAGCACATCGCATAGTTTGAAATCCCACAA AAAGACTCACCAGCGACAATTGCAAAACAAGGGTCGCAAGAAAAGGCCACTAAAGACGCAGCAGACCAAGTGCAGCGATC AGGAGCAGAAGGATCCACAGCAGGAGGAGCAAGAGGAAGAGGAGTTCATTAAAGAGGATCAACCCGAAATGACGTTGTTA AATCCAGGCAGTCACTGCTCCGAAACCACCAGCACGGACAGCGGAGTGGTGCTGCAAACTCTCACGCCGCAGGATCACTT GTCCAATGTGTTTATCCTACAGGGCAACGAGCCTCTGATCCTTCCAGAAACATCACAGGCTTACCAGCTCTCCTACGCCG CCGAGGAGGAAATACCCAGTCCGTGGATTGACGCCGGCGTCCTTGTCTCTAAGCCCATCATTCCCATGGCTCCGCTGACG GATGCCTGCGTGGCCTTGCCCACCGAGATGCCCAGTTTCGTTAACCTGAAGCCCACATTCGGCAATGCAGTAAGCGGCAA TATGGGGGATCCGCAGCCGGAGACCATGGATGTGGATCCCACAGTGGAAACCTCACTGCCCACGCCCACCTTGGAGTTGA ATCAGCCAAATATCGAAGAGCTGCTCAAGCAGGACAGTTTCGAGAACGAGGAGGACATGGAAACGGAGTCGCTTCTAAAT GACATCCTCATGACCATCGACAACAACAGTGCGCTGCTGCAGGCCACACTCCAGCAGGCCTCACAGGTGCCAAGCGATGC CTCCGGCTTGGTGGAACTGGACATACGGGACAACAAGCCGACTCTGAAGCAGATCACAGCGGATGCGGGCATCTGCAATT GCACCAACTGCAAGTGCGATCAGACAAAGAGCTGTCATGGGGGTGACTGTGGTGCTGCAGCTGCTCAACAGACCACCAGG ACTGGCGCACCCACAACAACAACAACGACAACAACGATGAATTCCAGCGGTGGCAAACGAATTTGCGGCAGCGTGGTGCC CACCAAAAAAGTGAGTAAACGCGAGACGGAAATGAACCAGAACATCGAGGATGTTGCTTTGCTGCTGCAAAACCTTGCTT CCATGAGTAGCGGTGGCTCCTCCGGCGGTGGCGGCGGTTGCTGCGGCGGTGGTGCCGTCAAGCCAGCACCCTCAGGTGGT GGCTGCTGTGGAGAACCCAAAGCTCCGAAGCCCGTGAATGCCGGTTGTGGTTGTGCCCGCCCAAGTGCTCCGTCCTCTGG TGGTTGTTGCGGCAGTGGAGTTGCTTCTACTCCAAAACCAACTGGTGGTTGCTGTTCAGGCAACCAAAAGCCAGCTCCAA CAGCTACCGCTGCCTCGTCACCCTCGTGTTGCTGCTCTGGCAAGGGCGAAGTCCAGGAGCCGCCGGTCATTAGCAGCGCT TCTTCAGCTCCCGCTGCCGCCGTCAAGGGCAACGCCTGCACATGCAAGAGTCCGGCTGAGGGCGTGGCCAACGGCTGCTG CGTAGTGATCTGCATTAAAACGCTGCAAGCTCTACGCAAAGTTCTGACGCGCCGGAATCTAAATCTAATGCTTTGTCCAC AGCAGAACTAAACTAAGAACTACCTATGTATGTACACTAATCCCGATGACCCAAACCAAATCCACGAGCCCAGAGAATCC GATCATCAGAAATTTGAAACTGGTATTGGGCCTGTAAAAAGTATTTTTCCCATTAAGAAACCAAAAAAAATTTTTACAAA AAATATCAAAGTGCATTTAAGTGAAACGATTCAATCATATGTGATCCCAGCGGAGGCTGGCCGCGCTATCCCAAAACCTA CTATAATCATTGTAAACCCAACATTATCTAGAATCCCAAAGTAGCTTCTAGTGGAGCAGACAGAGAGATAAATCAAACAT TGCCATAAATAAAGTTTGTGAGGATATGTGTCGCCTTATCGGAAAATAAAACATGTCAGACTACGAAGTATACGCCCAAT TGATACACATGCACAACTGGACAGTTGATAAATGACCGTCTGTTCCCTTTAATCTAAAGCTCTTACTTGTTTTTTGAATC ATTTTTAATATTTAACACGTTGAATATCTTTGAGTTTATCTACAACTAACAAATGTAATTTATTACCACCCCAGATCAAA AGCGATCCATGCCTTAGGGACGTAATAATATATTTATATGTGGGGGACTTTCATTTGTTAAACTGTATTAATCAAAAATA ATAGAATACTATTATACTATATTATGTTTTTTTGTATGGAGTTTATGACGCTTTATATCATGCCAAATATGAACAGAAAC ATATTTTCTACTGTTTTCTTATATACCCAAGTTAAATCATTTGTATAAAGTCGAATAAAACATAAAACTAAAAAAAAAAA AAAAAAA
Clones isolated by an inverse-PCR screen (SLIP) using a mixture of the GH, LD, LP, and SD cDNA libraries.
Adult heads were collected from an isogenic y; cn bw sp (iso-1) strain.
These clones have been isolated a directed screening process called SLIP (Self-Ligation of Inverse PCR Products). Clones isolated from this screen have been PCR-amplified using a mixture of 4 different plasmid cDNA libraries as template. These include the GH, LD, LP, and SD cDNA libraries.
