AGCAACCAACAACAGCAGCAGCAGCAGCAGCAGCCACATTTCAGTTACAGCTCCAGACTCCCAGGTTGCAGACTCCCAAA GCAAACAGACTCCAGTCCACGATCCAGCTCCAGTTCCACCGATCCGATCCACCTCCAGCGTGCTCGAGTGCCATAGATCC TCACCAAGTGCCAAAATCCGCATCCTGATCCCAAGAGCTCAAGGCACCCCGGCCCAAAATTGAGCTGAGAACGAAACGAA GGAAGTTCCTTAGTGCCATAGAAAGCAGTTAATGAAACAACGACTAAGACGAAGATCGACCATCCAGAACCGGAGGGAGC TAATTGCGAACGAAAGAAACCACAAAGTGCCTTCCATCAATCCGTTGATAAGTGATATTTATTATGTTTATACTTGCCAG CAGCCGAGGCAGCAACAGCAATAGCAACAACCATAGGGGATCACGGCATCGATGATCAGTCCACGACCAAGTCCTAGTGC AATCCGGAATCCAGTTCAAATTAGTTCAATAAGCCGTATCTACCACGTATAATGTCCACATCCACCGCCACAACGAGCGT TATCACGTCCAACGAGCTCTCGCTGTCCGGCCACGCCCACGGTCACGGTCACGCCCACCAGTTGCACCAGCACACCCACA GCCGCCTAGGAGTTGGCGTTGGTGTTGGCATCCTTAGCGACGCATCCCTATCGCCCATCCAACAAGGCAGTGGCGGCCAC AGCGGCGGAGGTAACACAAACAGTTCACCACTGGCGCCCAACGGAGTGCCACTTCTCACAACAATGCACCGATCACCGGA CTCACCGCAGCCAGAATTGGCCACCATGACGAACGTCAACGTGCTGGATCTGCACACGGATAACTCCAAGCTGTACGACA AGGAGGCTGTATTTATATACGAAACGCCCAAGGTGGTGATGCCAGCGGATGGCGGGGGTGGCAATAATTCCGATGAAGGT CATGCCATCGATGCGCGGATTGCGGCCCAAATGGGCAACCAAGCCCAGCAACAGCAGCAGCAGCAACAGCAGACGGAACA CCAGCCGCTGGCCAAGATCGAGTTCGATGAGAACCAGATAATCCGGGTGGTGGGACCAAATGGCGAGCAACAGCAAATCA TCTCGCGGGAGATCATCAATGGGGAGCATCATATCCTGTCGCGAAACGAGGCTGGTGAGCACATTCTCACACGGATCGTC AGTGATCCCTCCAAGTTGATGCCCAATGACAATGCAGTGGCCACGGCCATGTACAACCAGGCCCAAAAGATGAACAATGA TCACGGGCAGGCGGTATATCAGACATCACCATTGCCGCTAGACGCGTCTGTATTGCATTATAGTGGCGGCAATGATTCGA ATGTAATTAAGACGGAGGCCGATATCTACGAGGATCACAAGAAACATGCGGCTGCAGCAGCAGCTGCTGCCGGCGGAGGA TCCATCATATACACCACATCCGATCCGAACGGAGTGAATGTGAAACAACTGCCCCATTTGACGGTACCCCAAAAACTTGA TCCCGACCTCTATCAAGCCGATAAGCATATAGATTTGATCTACAACGATGGCAGCAAGACGGTGATTTACTCCACTACGG ATCAGAAGAGTTTGGAAATATACTCGGGCGGCGACATCGGCAGCCTGGTGTCCGACGGCCAAGTGGTGGTCCAGGCGGGA CTGCCGTATGCCACCACCACCGGAGCCGGCGGCCAGCCCGTCTATATCGTGGCCGACGGTGCCTTGCCAGCGGGAGTCGA GGAGCATCTGCAGAGTGGAAAGCTCAATGGCCAGACCACACCTATCGATGTCTCTGGCCTATCGCAAAATGAGATTCAAG GCTTTTTGCTCGGCTCACACCCCTCGTCATCGGCGACGGTAAGCACAACCGGCGTTGTCTCCACGACAACGATCTCGCAT CACCAGCAACAGCAGCAGCAGCAGCAACAGCAACAGCAGCAGCAGCAGCAGCAACACCAGCAGCAGCAGCAACATCCCGG CGACATTGTTAGTGCCGCTGGCGTGGGGAGCACGGGCTCCATTGTCTCCTCTGCGGCGCAACAGCAGCAGCAGCAGCAAC TAATTAGCATCAAACGAGAGCCCGAAGACTTGCGCAAGGATCCCAAGAATGGCAACATTGCCGGTGCAGCAACAGCAAAT GGACCCGGTTCGGTCATAACCCAAAAGTCCTTTGATTATACGGAATTGTGCCAGCCGGGCACGCTGATCGATGCCAATGG CAGCATACCCGTCAGCGTGAACAGCATCCAGCAGAGAACGGCGGTCCATGGCAGCCAGAACAGTCCCACCACATCGCTGG TGGACACCAGCACCAATGGATCCACGCGATCGCGGCCCTGGCACGACTTTGGACGTCAGAATGATGCCGACAAAATACAA ATACCAAAAATCTTCACAAACGTGGGCTTCCGATATCACCTGGAGAGCCCCATCAGTTCATCGCAGAGGCGCGAGGACGA TCGCATCACCTACATCAACAAGGGTCAATTCTATGGAATAACGCTGGAGTATGTGCACGATGCGGAAAAGCCCATTAAGA ACACCACCGTCAAGAGTGTGATCATGCTAATGTTCCGCGAGGAGAAGAGTCCCGAGGATGAGATCAAGGCCTGGCAATTC TGGCACAGTCGTCAGCATTCCGTGAAGCAGAGAATCTTGGATGCAGATACGAAGAACTCGGTTGGCCTCGTTGGCTGCAT CGAGGAAGTGTCGCACAATGCCATCGCCGTCTACTGGAATCCGCTGGAGAGCTCCGCCAAGATCAACATTGCGGTTCAGT GCTTGAGCACGGATTTCAGCAGTCAAAAGGGAGTTAAGGGCCTGCCGCTGCACGTACAAATCGACACATTTGAGGACCCC AGAGATACGGCGGTCTTCCACCGCGGCTACTGTCAGATAAAGGTCTTCTGCGATAAGGGCGCCGAACGAAAGACGCGCGA TGAAGAGCGGCGGGCCGCCAAACGAAAGATGACAGCCACGGGCAGAAAGAAGCTGGACGAGCTTTACCATCCGGTAACGG ATCGGTCCGAGTTCTATGGCATGCAGGACTTCGCCAAGCCGCCGGTGCTATTCTCGCCCGCCGAGGACATGGAGAAGAGC TTCTACGGCCATGAGACTGACTCGCCGGACCTGAAGGGGGCCTCACCGTTCCTGCTCCACGGCCAGAAGGTGGCCACGCC GACGCTCAAGTTCCACAACCATTTTCCGCCCGACATGCAGACCGATAAGAAGGATCACATACTGGACCAGAACATGTTGA CCAGCACACCCCTGACCGACTTTGGTCCGCCGATGAAGCGCGGCAGGATGACGCCGCCGACCTCGGAACGCGTGATGCTG TACGTGCGGCAGGAGAACGAGGAGGTGTATACACCGTTGCACGTGGTGCCGCCCACCACGATCGGCCTGCTAAATGCGAT TGAAAACAAATACAAAATCTCAACAACGAGCATAAATAACATTTATCGCACAAACAAGAAGGGGATTACTGCGAAAATTG ACGATGACATGATATCGTTCTACTGCAACGAGGACATCTTTCTGCTGGAGGTGCAACAGATCGAGGACGACCTGTACGAT GTGACGCTCACGGAGCTGCCCAATCAGTAGCGCTGGCAGTACGGGTAGCACCCGCTAACCGCACTCAAAAAAAAAAAAAA AAA
Clones isolated by an inverse-PCR screen (SLIP) using a mixture of the GH, LD, LP, and SD cDNA libraries.
Adult heads were collected from an isogenic y; cn bw sp (iso-1) strain.
These clones have been isolated a directed screening process called SLIP (Self-Ligation of Inverse PCR Products). Clones isolated from this screen have been PCR-amplified using a mixture of 4 different plasmid cDNA libraries as template. These include the GH, LD, LP, and SD cDNA libraries.
