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FB2026_01
,
released March 12, 2026
Comment: 0-22 hr AEL
CCGATGTCTCCACCAGCTCCAGTTCTTGACGCGTTCTGACACGGACGCGGTTTTCAGACAGAGCATTGAATTGTGTGTGT TGCTTCTTAATTCGCTTTGTTCTGTGGCCAATTTTATTGGGCCAACTGCGAAATCAGTTTTGTGCGGTGTCGGCCAAAAA AAAGAAGAAAAAGTGTCGCAAACAGCCGCATTAGCATAATTGCAAGCCGCCTTCTGCATACGTGTGTGAGTGTTGGTTTG TTTGAAAGCATCAGCAGAAAAGGCGGCAAGGAAAGAAGCAGAGAGGCGAAGCGGAGCGAAGAAAACAAAAACAAAAAAAA CTGCAACGGCAAACATAATTATTTGCGCAATGCATTCAACGTGAAGTTGCTCCAAAAACACCGACACTCGGGAAAAACGC ATACAACGACGAATCGGCAATTCATTTTACATGCTTTTTCGCCGTTTCCATCAGGAATTGACTGCAATACAGGCAAAACC GTTTAACTTGACCACCAGACGTCGAAAAGAATATATACGAAAGCCTTTTGTGCGCGCGACGCAATTATCTGACAGCACTT GAAAGTATGCTACACAAATTTGTTTCAACTTGTTACTGCAACAATAACAACAACCAAAACAACGGCAACCATCCCACGCA ATTTATCATATAGACTGCGTAGCAAGCAAGAGCGAGACAGCAACACGGCGCAACCAGCAGCGAGAAACAACAACAACAAG CAGAAATCAGGAAACCAGGAAACGAACTGGAATATATAGAAAATTCTACAACAAACTACAACAACAAAGCAGTAAAGCTG TTACTCCTAAAGGTAAACGGAGCCACCGACGGCACTTACAACATAAAGATGGCGTCCTCACCGACACCATCTCTGGACTC GATGCGGGGCGGGGCGAACTCGATTGAATCGTACGAACACGCCGGCTATCTATCAGACTCTCCGCTCACGTTGAGCGGTT CATCGCCACCGGCAAGCGATTCGGCAATCTGCAGTGATGAGTACACGGGCGGCAGCAGCGTTAAATCACGCTCTGAGGTG ACTGTAATCAATGGCCATCATCCCATTTCGGCCTCCGTATCGTCCAGCTCTTCGGCCAGCTCTTCATCCTGCTCGTCGTC ATCGTCCTCATCCTCGTCTTCGTCATCGTCCAGCTCCTCGACCAGCGGCCTCAGCGGTTGCGGCAGTACCAGCAGCAGTG TGATCAGCGCCAACAACGTTGCGAGCAGCAATGGACCAGGCGTTATCGGCAGCAATCTTCAGTCCTCAAACAATGGCGGC AACTCGGGCATCAGCAGTCTGGTCGTGGGTGCGGGCAAGGGAAGCAACAGCAGCAGCAACAGCTCCTCGAGCAACACATC CGCCAATGGATCTCCGCCACGTTGCACCGCCTGCAAGAGCAAGTGCAGCGATGCGGTGGCCAAGTGCTTCGAATGCCAGA GCTACCTGTGCGCCAATTGTGTAACTGCTCATGAGTTCATGCACTGCTTCAACGGACACAATGTGTGCCTGATTAAGGGT TTTGAGGCGAGCACCACTGGCACTCCGCTGTCGGTTGGATCGCCTAGCAACAATCCGGCGTCGAATGAGTTCAAGTACGC CAGTTCGCTGACCATGATGCTGCAGCAGCAGCAACAGCTCGACTCGCAACAACAGCAACAGCAGCAACAATTGCTGCCGG CGCAGCCTATGTCGCAACTCTCGAAGATTGTACTGGCAGCCGCCGCGCAGGCAAATTCCCAGGAGCAACAGCGAGAGGAT AGCATCTATGGTTCACTGCACCCACAACAACAACAACAGCAGCAGCAACAGCAGCAGCGACAACTCTTCTGCCCCCGACA CAAACAGGAGCTGCTCAAGTTCAGTTGCCGCACCTGCTGCATCCTGGTGTGCAAGGAGTGCATTGTACTGGAGCACTCCA CAGGTCTCCACGAACTGGAGAACGTGCAGTCCCCCGGAATGACAACCTCTACGGGATCCACAGCCAACGAGTCAGCTTTG CAGACTCTGCTCGCCGATATGCGTGGCAAAATTGGCGAGATTGTCGGCATTGCTGGAAACTCGGACCAGAATCTCACCAA GGTGAAGCTGCAGTACCAAAAGGCTCACAACGAGCTGAACGAGACGCACCAGTTCTTCGCTTCCATGCTGGACGAGCGCA AGACCGAATTGCTCAAGGAACTGGAGACGCTGTACACGGCCAAGGTTAACAGCAACAATTCGTGGCAGCAACGCTCCCGC GACTTGATCGACAAGGGACTGGCCACCTGCGAGGCGGTCGAGCGGAGTCCTGCTCCGCCATCATCCTTGCTGACAGAGGC CCTTCTCCTGCGCAAATCCCTGGAGCAACAACTACAGACGGGCATTCAGGAGATGCAGCTACCATTCGAGATCGAATTTA TGTCCAACTATCAGTCGATTCAGGCCGGCGTGCGCAACACCTTTGGTTACATTCGGGCAAACAGCTCAGATGGTGGACCT ACTGGCATGAGCCTGACGAGCAATGGCCACGGAAAGCAGCCGCCAATTGCCCGACCAACACAGAGCGCCAGCAACAGCAG TGCGAGCAGCGCGGGCAGTGGTCACCATGGCCACCACCAACAGTCACACCATCATGGCCACCACAATCATCACCAGACGG CCCATCATCAACAGCTGCAGGCGCAATCGTCGCTGCATGGCTTGGGTCTTGGATTGAGTGGCGCCAGTTTGTTGGATAGC AGCTCTAGCGCAGGCGGAGCAGTGGGAGCCTTCAGCAACGGAGGACTTCTGCTGGGCGGTCGCGATCGGAACGCTCTGGC AGTGGAACAGCATTTCGGTGAGCTGATGCCCAAGCGAGGAGGAGGTGGCTACACGGGCAGCAATGGATCGGCCACCAGTG CCGTGGCCCACTACAATCCCTACGAGAAGTGGAGCAACGGCGGTAGCGACAATCTGTTTTCATCGGTGACAAGCGGCGTG TCCGGTAGCTCTGCAGTGGCCGATGCATTTGCCAGCCTGTCTGCTGTTGGAGGATCCGTGGTTAGTGGCGCTGGAGCTGG CGGAAGTACTGTGAGCTCAGAGTCGTTGCTGGACCTCACCAACAAGCTGCTTTCGGCCACTATCTATCCGCCCAAGTCGC AGATCAAGCGACAGAAGATGATTTACCACTGCAAGTTTGGCGAATTCGGCGTGATGGAGGGCCAGTTCACGGAGCCCAGC GGTGTGGCGGTAAATGCCCAGAATGACATCATTGTTGCGGATACGAACAACCATCGCATTCAGATCTTCGACAAGGAGGG ACGCTTCAAGTTCCAGTTTGGCGAGTGCGGCAAGCGTGACTCGCAGCTGCTCTATCCGAACCGCGTGGCCGTGGTGCGCA ATTCCGGCGATATTATTGTCACCGAGCGCTCGCCCACACACCAGATACAGATCTACAATCAGTACGGCCAGTTTGTGAGG AAATTCGGGGCCACCATTCTGCAGCATCCTCGCGGCGTGACCGTGGACAACAAGGGACGGATCATTGTGGTGGAATGCAA GGTGATGCGTGTGATCATCTTTGATCAGAACGGCAATGTGCTGCACAAGTTCGGGTGCTCTAAGCACCTCGAGTTCCCCA ACGGCGTGGTGGTCAACGACAAGCAGGAGATCTTCATCAGCGACAATCGGGCGCACTGTGTCAAGGTGTTCAACTACGAG GGCCAGTATCTGCGGCAGATTGGCGGCGAGGGTATTACCAACTACCCAATTGGCGTCGGGATCAACTCGAACGGGGAGAT CCTCATCGCGGACAACCACAACAACTTCAACCTGACGATCTTCACGCAGGACGGGCAGCTGATCTCGGCGCTCGAGTCGA AGGTGAAGCACGCGCAGTGCTTCGATGTGGCGCTCATGGACGACGGCAGTGTGGTGCTGGCCAGCAAGGACTATCGGCTC TACATATATCGCTATGTCCAACTGGCGCCAGTGGGTATGTAAACACACAGACACACACACTCCATGGATGTGAGTCCGGA AAGGAGGAGGATCTGTGGTCCGGCACTCACTACTTCTATAGGCTAAGTTCTCAATAGTCTGACTTGCTCGGTCTCTCCAG CTCTAATTCTAAATCTACACAATCGAAGTTGAAATCGAAATCTATAAGATCCCTACTATCTCCCCCGATCTGTCATCTCT CATAGCCTTAGACTTTGCTCGCTATGTAATAACACACACGCACACTGTTGGAATGATTTTTATTATCTTTTAAATTATTG TTGAATTTTGTTTTATTTTTTTTATATATATTTACACTGTTAAGCTTAGTTTTTATTTGTGCGTACTTGTTTATGATAAA TATATATTATGTGTATTCATGTTCATGTTCAAATCGGATAGTGCCGCCGCCTCTCTATATCTTTGCACACTTCTCACTTC AAACCCTCGTATCTCTATCTCGCGTGCAAGTCGTATTTTCAACAAACACAAGCAAACAACCGAAACAAACAAAAAAAATG ACACTTTTTAACTATATATAGTAGATACCAGAAGAAATCCCTCCACTTTTTTTATGTTCGTTGCCGACTGCAAGAAAAAA AAAAAAAAAAAAA
Cloned cDNAs prepared from polyadenylated RNA isolated from 0-22-hour embryos.
Embryos at 0-22 hr AEL were collected from an isogenic y; cn bw sp (iso-1) strain.
RNA was poly(A)+ selected twice. cDNA made using Stratagene ZAP-cDNA synthesis kit; oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. For clones LD21101 and higher, cDNA directionally cloned into EcoRI/XhoI-digested pOT2 plasmid.