TAACACTGACCAGTTTCTCATCGTTAAAAAATCCATTTGGAATTCGGTTTATTTAATTAAAAGCAGCCGGGACACCTATG CGAAGGGCAAAAAGCGGCCAGAGTTGTTGTTGCTGCTGCTGCCCAGCCGCCGTGCGATCGATCGGGTCTGGCGGCCCCAA CTAAATAATGTGGAACATGATGTGCGACGTAATGCCCACGATATCCGAGGATAGCATCTCGCAGCGCTCCTCGCAGTTTA GCGGGGAGGATGCCAACTTTGAGCAGCTGATGGTCTCCATGCTGGACGAGCGGGACAAGCTGATGGACAGCCTGAGGGAG GCGCAGGAGCGGCTTAACGAGACGGAGAACAAGCTGCGTGACGTCGAGAAGGAGCGGGACAGCCTGCAGCGACAGATTAA CGCTAATCTGCCGCAAGAGTTTGCCACCCTGACCAAGGAGCTGACGCAAGCGCGGGAGACCCTTTTGGAACGTGACGAGG AGATTGGGGAGCTCAAGGCGGAGAGGAATAATACCAGGCTTCTCCTCGAACATCTGGAGTGCTTGGTGTCCCGCCACGAG CGTTCCCTGCGTATGACCGTTGTGAAGCGACAGGCTGCCGCCCAGAGCGGCGTCTCCAGCGAGGTGGAAGTGCTCAAGGC GCTAAAGTCGCTGTTCGAGCACCACAAGGCGCTGGACGAGAAGGTGCGCGAGCGGTTGCGGCTTTCCATCGAGAAGAACA ACATGATGGAAGAGGAACTCAGCTCTGCCAAGGAGGAACTGGCCCAGTACAAGGCGGGCGTGGTGCCAGCTGGCGTGGGC AGTGGCAGCGGAGCAGGGAGTGCCGCAACCACCGCCGGTGGTGGGGGCGCCGAGAACGGGCTCAAAGAGAAGATGGCGGG CGTGGGCGGATCTGGTGGCGTTAATGGCGAGGCAAATGAGCTGAACGACTACGCAGCCAAAACCCACGAGCTGCAAACCA TTATTGAGAAGCAGACCTCGGAACTTTCACAATGGCAGCGACGCGTTTCTGATTTGAACAACAAGATTAGTGAGCTTGAG GAGAACATGTCGCGAGTGCAAAAGGAACACTGCAAGGCCCAAGACCAGTGTGCCAAGCTGCAGCGGGATCTGCGCGAGAA TGTTGCCCAGAAAGAGGATCAGGAGGAGCGCATTACCACATTGGAAAAGCGATACCTGAATGCTCAGAGGGAGTCTACAT CGTTGCATGATCTCAACGAGAAACTCGAGCAGGAACTACGGCATAAAGAGGCGCAGCTCAAGCTGCACGAGGAGAAGATC GGGGCCATCGAGGAGAAGCTGGAGCTCTCCGAACAGAAGCTCGCCCAGCACGCCAAGCTGCAGCCCGACATGGAAGAGCA GCTAAAGGCACGCATGGAGGCATTGACTAAGGCTCAAGAAAGACACGGTTCTGCAGAAGACCGCATCCGGGGACTGGAGA CGAACCTCGACGAGAAGACCAACGAGGTGGTAAGACTGAACCAGCGTCTGAAGATGAACGAGGAGCACAACCTACGGCTT TCCTCCACGGTGGATAAGCTGCTCTCGGAGTCAAACGAGCGTCTGCAAGTGCACCTGAAGGAGCGTATGCATGCGCTGGA CGAGAAGAACGCCCTCACCCAGGAACTGGAAAAGGCGCGTAAGGTGGCCGAGGAGCTACACCACGAGAAGAGCGAAATCA TGAAGGAGCTTTCGAAGACGCGCCTGGAGATCGAGAACTTTAAGCGGCAGCTGCTGCAGCAGGAGATCGCCTACAACATC CAGCAAACGGAGGCGCTCACGCGGAGCCTTTCGCCCAGTAGCGTAGTGGACCCCAGTGGCGCCTTCAGCCGGAGCAATTC GCACGCCAGCTTTGAGACGCACTCCCTGCGCCGCCAGAGCAAACAGCGACTGTCCGAGGAGAACGCCCTGGTCCGCTCCA TGGCCGAGCAGGAATGGGAAAAACTGCAGCAGGCGGCGCATGCCCAGCAGCAGGCCTACGAGCTGGCCAGCGCTGCGGAT TGCGATGACTCTGATGTCCTGTACGCAGCGGCCACCGACATGATGTCTCCTTCGGGACACACAGATGCCCAGACATTGGC CATGATGCTGCAGGAACAGTTGGACGCCATTAACAATGAGATTCGCCTGATCCAGGAGGAAAAGCAGTCAACAGAGGCGC GGGCCGAGGAGCTGGAGTCGCGTGTCGGCAGTCTGGAGCACGTAAACCTGCTGGCTCGCGGTCGCTCCATGGACAGGCAG AGTCCGCCAATGAGTGGCCGGAGCACGCCCAACAGTCCGCAGCGGGATTTCATGCAGAAGTATCACACGCTCAACTTGCC CGTGCTGTCCAGCGATGCCTCTCGCGAAGAGCTCCACGGCGGCATGTCCACCACAGGTGATTCGAGTTCCGGCGGAGCGG CCTCTCCACTGACCGCCCGCTCTATGCGCCTGGAGCGTGTGGCCCAGGCCTTGGCCCACAGCCAGGAGGAGCTAAGGCGG CGTTCCATTGGCCTTAATCCCAATGCTTCAGTGGCGCCCAACCACACTGGCGGTCACATGCCCCTGAGCAGCCACAGTTA CGGACTGTCGCCGCTTAGCTCTCGTTACGGCAGCCAGGAGTCACTGCGTCACTATAACACAATGGGATCTATGTCCATGC TGCAGACACCTACTTCGGGCGTATCCAGGGAAGCGGCAGCCGCTGCTGTCCAAAAGAAGAAGGGCATTAAGTCCTCGCTG GGTAGGTTCTTTAGCAAAAAGGAGAAGGTGAAGGGCGTGAAGGATACGCTGCCCGACGGCTCGCCCAGCATGATGTCCAT TGGCAACCTGTCGATTGGACTGAGCGAGGTGGACTCCAACTATGATGCTATGAGCATGACTGGCGGAATGATGCCCCGCA TTGCCAGCTCCCAGGGATCCAAAATAAGCAGCGTTGACTACGGCAGGCAGAAGAAGGAGCATGATTACCGCAATGACTTG CTGGGGGAGGCCATGAAGGCGGGCACACCGTTTGCCCTGTGGAATGGCCCCACCATAGTCGCCTGGTTGGAACTGTGGGT GGGCATGCCAGCCTGGTATGTTGCCGCCTGTCGAGCCAATGTCAAGTCCGGGGCCATCATGAGTGCGTTAAGCGATACGG AAATTCAGCGGGAAATTGGCATCAGTAATCCCTTGCACAGGCTCAAACTTCGGCTGGCCATTCAGGAAATGGTTTCACTG ACCTCTCCCTCGGCGCCACAAACCTCTCGCACCACTCTGGCATTTGGCGATATGAACCATGAGTGGATCGGTAACTATTG GTTGCCAGGTCTTGGCCTGCCCCAATATCGCACAACCTTCATGGAGTGTCTGGTGGATGCCCGGATGCTGGACCACCTCA CCAAAAAGGACTTGCGTGGTCAGCTCAAAATGGTGGATAGTTTCCATCGCACCAGCCTACAGTACGGCATATCGATGCTC AAGCGCCTTAACTACGATCGCACGGAGTTGGAGCACAGGCGCAAGATGAGTGAAAACGGCCTGTGTGACGTACTAGTCTG GAGCAACGAGAGAGTTATCCGATGGGTTGGCAGCATCGGATTGAAGGAATATGCTAATAACTTGCTCGAATCGGGAGTGC ATGGAGGATTGATGGCCCTGGACGAGGGTTTCGATGCGAATGCCATGGGCTTGGCCCTTCAAATACCTACACAAAATGCT CAGGCTCGCCAAATACTTGACACGGAGTTTAATAATCTGCTGCAGATTGCCACAGATCGCAGGCCGGAGAATGAGCAGCG CAGTGCTTCCTGATGCAGCTACAGCCCGCCCACGTCCTCGGTGGATGAGTGAGCACCAGCCAAGGGGGGCAGGACGAAAA ACAGACAACAAAGGCAACCTACAACAATAATAACGTAAAGACCAGGGGCAAAGTTTCCTAAACAGCAACAGCAACCGTAA CGACAAGCTGAACTAGAAGAGATGAATGAAAATGCTAGCAAGCAACTAAGCCACAAAAAATCTAACAAGAAAGACGCAAC GCAAGAGACTCCCCAGCATGATGCACGTCTAATCCACATATTTAATATATATAAATACACGCATAGTCACATAATCCATA ACAAGACAATGTATACAAGACATAGCACATACTCTACATAGGGCATACGTAGTTGCATTGGCGCCAAGTCGCTGCCGCTG CCAGGCGTGTACAAGTTTTAGAAGCTAATCAGTTTTCGATTGTAAAATTATTATTGTATTTTTTTATATAGTGTAATTGG GCTTGCAATAGACTAACAGTATTTTGTTTTAGATGATGCAGAGGTGGAAAGAGATGACAGCGTGCTTCGAACCAAATTCG TAACCAAACTTGTTTGTTATATAATTTTTATGGCATTTGTACAGTTATTAATTTAATCCAATTTTAATTTCTTTAAAATT AAAACAACTAACATACGCTTTACATATGAAAAAAAAAAAAAAAAAAA
Full-length open reading frame fused to a carboxyl-terminal FLAG-HA tag cassette under the control of a metallothionein promoter.
A cDNA sequence encoding each bait molecule was cloned into the pMK33-C-TAP-FLAG-HA-BD vector as a full-length open reading frame, fused to a carboxyl-terminal FLAG-HA tag cassette under the control of a metallothionein promoter. cDNA sequences are derived from previously characterized and publicly available clones created by the Berkeley Drosophila Genome Project; all inserts are full-length sequence-validated.
