GCGCAAAAATTGGTCGGCAAGCAATTCAAAAGTAAGAGCAACCGGAGCGAACAAAAAAGGGAATAACTTACTAAAATCTC TGAAAGAAAAATAAAAAGACTAACGGGCTCGGCAAGCTGTGTTTATTTCGACAAGTAATTATATACTTGGAGTGCAAGCA AAGGCGAAGGAAGTGGAAGGACAAGCAACGAAATCGTGCTCTTATCCGTTCCTGTACTGTGTCTCTCTTTCGCTGGGAGA GTGTGTGTATTGGTGTGAGTGTAGAAATCTGCAAGAACAGCAACGCCAATAAAAGTGGAATCGAGAAAAAAAACGCAGTG GCGCGTGAATCACGAGCAATCTGAATCATCTCTCTACAAAAATACCTGTTTCTGTTGGCGCATCATTTATACCAATTAAA TCCTAAAGGATGGAACACCACGAGGACAACGCGCAGTTGGACAACTACTTGCAGAACCGCCTGGCCGAAAGTCTGCAAAT TTGCGGAGGCGCCGGCGAGCATAATCCTCATCTGGCAGACGCTACTGGGGGGAACGGCTGCGCTCCTGGCATTGCGCCCT CAAAGTCGGACGAAGTCGACGGGGAAGAAGACGAGGAGTGGAAGTACATACACGAGGTGCGGCAGAGTGAAAAGCTTCAA CAGGAAAAGCTTCCACTGACAAAGGAAACCGGCAATGGCTTCGGGCCCGGACGGGACTCAGATAATCAAGTTCACGGAAA CGGAGCTGCCGCTGTCTTCAATCTGTACGAAGAGGACGTGGAAGTGATCAAGAACGATGGTGACTTCTCGACAAATTCTA ACACCACTACTTCAACGGACGAGGTAGTTGCCAGGCAGGCCCAGGAGCCAAACCAGTTGCCGGAGCAGCTTCAACAGCAG CAGCAGATAGAATCACAAGGTGTTCACGAGGATCCCCGTCAGGAAGATGAGGATGAGCACAGCTCCGTGGCCACTACTTA TGGAACAAGCAGTCTCAGTGAGAACAACTCCTCGCCTTTAGATCAGGAGGAGGTCGTTATGGTGGCCCAAACGGTTGGTC AAGAGCAGTTAGTGGACTTCGACAACAAGGAAAACTCCTATTTTGTTAAAAACCTGGAAGAAAACCACTCGCAGCTAAAC CCCAATGCTGTGGCCTTTGTGCCTGGCGTTGGCTCACAATCATCCTCTCCCCTTCCTGCTGCTGAAGATCCACTACCTGG TGTTCAGCCCCGCCCATTTCTACCGGGAGGGACCCTTGACGATTTGGTGGCGGAAAGTCCGCGGAAAGAATTCGCCCGAA TCAACATGGATGGTATTGCCGTGCCCGACGAGCGGGAATTTGACATTGAAGCGGATATGCGACCCCACGAGTTGGAGCAG GAATCGGACACTTTTGGGGCAGGACACCTGGAGATGCAGTTGTTGAACGGTATAGGAACCGCTGACCAAGCAGCCTTAAG GGATGTTCTGGATCACGGTCCAGAGACAAGTGTCGATATGGAGTTGCCACTAGATCAGGTGCCCAACGACGCCGACATCA TGAAACAATCGATTTATGCCGAGCACAACTCCTCCATTGAAGATATCTTAAATTCCGTGCAACCTTTGCCAATTCAGACT TGTGACGATAAGGAACTAATTCATGTGGAAGAGAAAGAACATGTCTCGAAGTCGCCTTCTACAGAGGAGCTCCAGTTTCA GTCCGACTTCCCAAACAATCAGGAATCGCACACATTATTCAATAACACTGAGCAGGATCCAATGCAGGCTTCATTTTATT TGGAACATACCTCTCAAAAGGCTCAAGAGGGCTGCCAGGAGCAAATGCAGCTTCCCGCTGAGTGCTCTGATATTTTTGCC GACCAGAGTTTATTGCTAGACACCAGTGCACCCCAGTTGAGCTCCGAAGCAGACTCGCCTGTGGCGAAATTGGAGCTGGA ATCCCAGCAAGCTGGCATTGTAGACATCACACCTTCGCCCCTTTCTTCCACTGCAGAGAAACATCTGGTTGAAGACACCA AGGAGCTAGTTGAGGAGTACACGTTGGATCCGGAATCCCATTTCTTTGGGGTTGTTTCATCTCAAGCTCCTCTACAGAAC GACGAGGAAAATGCTGTTTTTGAATCAGTTTCTGGTTATGAAACTCAAAACTTTGATGAAATATCATCTCCACCGGAAGG TATTAATCCGTTTGCCCAGCCCTTCACGCCCGCCCACCTGGTGATAGAGCAGGCGAATACAATGATGGAGGATGTGGGAG GTATGCCTATACCAGCTTCCGAGGACTTTGCTATATGTGATAAAGTTGCTAGTAAGTCTTCAAATGAAGTAGAGGATCAT AGGAGTGAGCAACAAGCCTTTGTAAAGGAGGAGCTGCTGCACCCTGTTGGAGATGTTGTTGCCCAGGTTGAAAATCTTGG CACTGAAAAGAACTTCGTTGTTGAAGAAGAGCGACTTCCTATTTCTGTTTCAGATGAGATACCGCTTTCTTCGGCTTCAA AGGAGAAACTGTTGCCTGATACTACAGATGAGCAACTGCTGACTTCTGCTTTGGAGGAGAAACTGCGCTCTGTTGCTCCA GAGGAGTCAGTGTCAACTGCTGCCGATGGGCAGTCAATTTCTCAGTTTGATGAATATGTTATCGCAAGTAATAAACCTTT GGAAGATATTTTAGAACCGGAAAAAGATGTCGAAGTTGCGAAAAGTTTGTCTGAAAAAACTTCTGTTGCTACAGTGGCTG GAGGTGCTGTGGTTGGTGCTACCAAAACCCACTCAGCCACCAAAGCAGGATCTACAGCAGCTAGTGCAAAATCGAAAACA GAAACCCTTGTAATGAAGAAGACTACAGCGAGTTCTACTTCTGTTTACGGGGCAAACAAGTCGGCCGCTCCACGTCCAAG TACCGCCCGCCTTGGAATCAAAAGTACCAGTATAGCTACAAAAACTTCGACTACATCTTCACTAACTGGAAATCCCCGAA AGTCACTAAGCAGTAATGTAGGATCAACAGTTAAACCACCAACCAAGTTAAGCGGCACACGACCAGCTACAGCTCCAGTG AGCAAGGTAACTTTAGGGGCCAAGACCATAACTAACAAGCCGACTGCAAGTGGTACTGCATCTGATAATGTAACCAGGAC AACACTTCGCCCTTTGGTCAGTACCAACGCTCGCAGACCAGCAACAAGTGGAACAGGATCTGTAGCCTCCTCAACTGCTC GACGACCAGTTACAAATGCAAAAGGATCTGCTCCTGGCAGCGCTGCTTCAACAAAAGTTCGCCCTGCGGCCACAATGACA GCCCCAGTCAAGCCTAAAGTTTTGTCGCCGCGCAGCACCATTTCCTCCACCACCACTGTGCGAAAGGTGCCTAGCACAAG TACCCCTTCATTTTCTACTCGTAGCCCTAACAAGCAGCAATCCAATGGATTGGGCAAGAATACCAGCTCTACCACCACTT CCACTGCTACCGCAACTATCACAAAATCTTTCACAGCTCGCTCAGCTCCAAAGTTTACGCATTCAGCTAGCTTAACCTAT AACAATGGGAGTACATCTCGTCGTTTGCTGGTTCCTGGAAGCTCCTCTACTACAACTACGTCCTCTCTTAGGAAGTCATC ACCATTAAAGGCAGCACCAGGCAAAGCTGCTTCCAAGCCATTGACACCGCAATCCAAAGATGGCACGGCTAAGTCATCGC CGGCTGTATTAAAAGCTCGGAATTCTACTTTGATGGGCGGGGAAGGGGTAGCACCATCAAATGAATGTGTCCCAACACCT AATGGTCAGATCAATGCGGAAGAAGTGGTCAAGCTTAAGGGAAAGGGCCTAGCAGAAGAGGTTAAGATTATTGAAGAGCA AAAGCTTCACGAACAAGATGTCCCTGCCCATAATGCTGAAGTGCCCCTTTTGGATTTTTAATATGTATCCTAAATTCCAG ATCACACAAACCTAAAATTACTTTATTAATACTGAAAACATATTGAGAAAAAGTGTTTAAAACGAACAGAAAAAAATTTC GAAGACGTAATAAGAAAAACGATGAATACAATAGCATCCTGGTAACCAAATTAGGAATTTTTGCTTATTGAACTTATTTA TATACGATTATACCCTCGATTTCTTGCTACATATTATTAAATCATTTTATTTCAAAAAATAATGAACTACTGTAATGAAC GCAGAGCGAATAAATGTTTGTGCTATTTTGTAACGAAAACGAAAGATAAAAAAAAAAAAAAAAAA
Full-length open reading frame fused to a carboxyl-terminal FLAG-HA tag cassette under the control of a metallothionein promoter.
A cDNA sequence encoding each bait molecule was cloned into the pMK33-C-TAP-FLAG-HA-BD vector as a full-length open reading frame, fused to a carboxyl-terminal FLAG-HA tag cassette under the control of a metallothionein promoter. cDNA sequences are derived from previously characterized and publicly available clones created by the Berkeley Drosophila Genome Project; all inserts are full-length sequence-validated.
