ATCCGCATTAGTTTTTTTTCGGGTCCAAAATCTGTCCAAATCCGCAAATTGCACGCATATTCTAGTATCTGAAAACCAGA AATTTAGATAGACTGGGTACAGAGTCATCTGAAAAACGCAAAAATTAGGCAATCTCCGAGGAGCAACGTCCTTAAAACGA GAACGCATTACCCACTTTTGAATGCCTGTAAAGTATTATGGAAAGAGGATGCCCAGCTGCTAGCAGTGAATCCGTGACCA GTGCAGGGGAGCGTACTCAGTCAGCAGTCACCAGCTCCACCTCCACGTGGGTCAAGAGTCAGGCCTCAACCAGCCGAAAA ACAGAAGCATCAGAAGAATCGGGATTAGGAGCAGTAGACGCAGAAGTAGGAGCCGGCAGAGAAGCCTTTGTATCTATGTC AACGCTGCGGGAGGATGTGGAGGACGTGTGTGTGTCGTCAAACTCACAACATGGTTTCGCCGTAGTTTTGGACGACGAGT CCAGCACATTTGAGATTAGCTCTTCCAACTCACTGCCCACCAGTGCGGGGGCGGCGTCCACAGTGGGAGTGGTGGCCGTC GACGACAGCTCCTCCACGGATACCTTGAACGGTGGTCATCCTGACTTAGGTCACCCCGCCTCATCGGAACACAGTCGTCA GGGCTTTTTTAATGAGGATAATGAAGATCCTCCGGTTGTATGCCTTATAAACGACGATGACGATGATGAGGAACCCGAGC CGGAGGAGGACGATGAGGAGGAACTGATTGAAGACGAGGACGAGGACGCAGTGGACATTGTAACGGGCGCCATCAGCTGC CCCAACACATCACAGTTGGCTTTAGCCGACGGCACCATCATGGCGGCCGATGGATCTAAGATCTTCCTGGAGACGCCCGT CGTGGAGGAGGCCCAGCCGCATCCCGGTCAGGTAGTCACAACGGGATCCCAGTCGGAGCTCACAGGCAAGCCAAAAAGAT TGAGCGATGAGTTCTTGCTGGGTGAGGAAGACCAAGCAGAGAACCTGGCTTTGGGAAGATGTATTAAGTCTGAGCCGGTT AATCCCGTGGACGACAATCCATCTGAAGGCGACGATGGGGCTACTTGCTTCTCACTACATGACCGTCTTATGTCAGTGCG CCTGAAGCAGATGAGCCTCACGGCCAACACCGTTTCGAATCCCTCGCCAGCAGCCTCTGCCAACGCCGCCGCCCCAGAGG AGGCCTCCACTTCCAACTCCAGCTCAACCTCCAGCTCTGCCTTGTCGAGGGCAGACATTGAGTCCATGGATCTGATCGAG CGTCGCGACTTTGAGACGGAGCAGCGTCTCACGGGCGGCATAATCCTGCGTACCAGCTCCATGGTGAGCCAGAACAAACT GAATCTAAGTCTGATCAAGTCCATGGCGGGGGGAAGCAAGGCGGCCAATGGATCGGGTACGGCCAACAGTGACGACTGGC CCAGCAGCAGCAATGGGCGGACCGTCTCATCGGACAGCAAGTACACCTACAAGGATCTGTCCACGACTCCAACGAGCAGT CGCAAGTACACGAATAGCCGGCTAAGCAAGTCCACTGCCAAATTAAATCTGGGATCCTCGTTGGGCGCCTCCAGCTGCAG CCAGCACCGGAGTGGTTCCAGTTCCACTTCAAAATCCATGGAGTCATCGACCAGTTGCACTGGAGCTGCCCGAACGGACG TTTACACAAACACCAACTCGAATGATTACCCCAGTCTGGCTCCAACCACGTCTGGTTCGTCCACATCCGGGGGAAGCTGT CAACAGGATCAGGAGGAGAATGTGAGTGCATCCGTCTCGTATAGCTCGGTGGGATCGCAGACCTCTCAGGAAAGTGGTTG CAGTCGCACCACTGCCATCAATCCAACAGCTGCCTGCTCCACCGGATCGGCCTGCTTGGGTGACTCTCAGGCCTCGACAT CGGCTTCTACCTCGTCCGGAGCCGGAGCGTCCAACCGTTGCCAGTATGCCACTACATCGACGACAAAGGCAGCTCGCCAG GTTAATGCCAGCGCCCAAACCCAGGAGCGTTTCCTGACTCGCAGCAATCCTCCAGCTGCTAGTGGAGCGGGGTCGGTTGG GGCAAACCCGACAGCATCTGTTCGGCAACGGCGAAACGGTTCCAGCGATGTTGTCCACCTGGAAGTGGTGGTGGAAGAGG GAGCTGGCGGAGGGGACGGCGGCGTGGTGGAGCCCGGCGACTTTAGTGCCGAGGAACCTTGGGCCAACTGCGATGAGGAG AACAACTGTTCCGATCTGGAGGAGATTTGCACATGCCAGAACGGTAACGGAAGCAGCTACGGCGGAAGCAATGCCAGTCT CAGCGAGACTTTCGACATGGACGCCATGGATCCCGATGAGCCCATTTCCTTGTCGCTGTCCTCTGCCTCAGCTGGCTTCA CCGAATACTCGCTGACCAATCCCAGCTCGCTAATGTCTCATCAGCGCAAACGAAAGTTCAACGAGGGACGTCTGCTGGAT GGCGGCGACTACTCGGTGACTATATCTTCCAGCGGCGAAGTGGGCGGACCGGGTTCAGGTGTGAGCGATAATTGCCGCAA GCGGATTGCCTATGATTTTGCATCTACGCCGCGCTCCTCGCAACATTTGGGACCCACGGCAGTGCTATCGGTGACGCCCT CATCGCATCTGACTAGCTCCACGCCGGGATCGGCTTTGGGTCGTCGCACCCCACGTTCCGTGCCGTCTCGTGACAATCCG CCACCAGAGCTGCAGCACTGGTTGGCACAATTCCAACGATGGTCCCATGTCGAGCGATTGCTGGCCCTGGACCGTCTCAT CGATCACTGCGATCCGTCTCAGGTGCGGCACATGATGAAGGTGATTGAGCCGCAGTTCCAGCGGGACTTCATCTCCCTGC TGCCAAGAGAGCTAGCCCTCTTTGTGCTCTCGTACTTGGAGCCCAAAGACTTGCTGAGGGCAGCGCAGACCTGCCGCAGC TGGCGCTTCCTGTGCGACGACAATCTGTTGTGGAAGGAGAAGTGCCGCAAAGCCCAGATCCTCGCCGAGCCGCGTAGTGA TCGCCCGAAACGGGGACGCGATGGCAACATGCCGCCAATTGCTTCGCCCTGGAAGGCCGCCTACATGCGTCAGCACATCA TCGAAATGAACTGGCGGTCGCGACCTGTGCGCAAGCCCAAGGTACTCAAGGGCCACGACGATCATGTGATTACGTGCCTG CAGTTCTCTGGCAATCGCATTGTGTCCGGCTCCGATGACAATACACTTAAAGTATGGTCAGCGGTGAACGGAAAGTGCCT GCGCACTTTGGTTGGTCACACCGGTGGTGTTTGGTCTTCCCAAATGTCCGGAAATATCATCATTTCAGGCAGCACGGATC GCACGCTCAAAGTCTGGGACATGGACAGCGGCGCATGTGTGCACACACTTCAGGGGCATACGTCCACTGTGCGGTGTATG CATTTGCATGGCAGCAAAGTGGTCAGTGGCTCGCGAGACGCAACCTTGAGGGTTTGGGACATTGAACAAGGCTCCTGTCT GCACGTTTTGGTTGGTCACTTGGCTGCTGTGCGTTGTGTGCAGTACGATGGGAAGCTGATTGTGTCCGGAGCGTATGATT ATATGGTGAAGATCTGGCATCCGGAGCGCCAGGAGTGCCTGCACACACTGCAGGGCCACACAAATCGCGTCTACTCGCTG CAGTTTGATGGCCTGCATGTGGTATCGGGTTCATTGGACACCTCTATTCGCGTGTGGGACGTGGAGACGGGCAATTGCAA GCACACCCTGATGGGTCATCAGAGTTTGACCTCTGGCATGGAACTGCGTCAAAACATTCTTGTCTCGGGCAACGCCGACT CGACAGTCAAGGTGTGGGACATCACAACCGGACAATGTCTGCAAACGCTGTCGGGTCCAAATAAGCATCATTCTGCGGTC ACCTGCCTGCAATTTAACTCCCGATTTGTGGTGACCAGTTCCGACGATGGCACCGTGAAATTGTGGGACGTTAAGACGGG CGACTTTATACGTAATCTTGTGGCTCTGGACTCTGGTGGATCCGGCGGCGTTGTGTGGCGGATTAGAGCAAACGACACTA AACTCATTTGTGCCGTCGGTTCCCGCAACGGAACGGAGGAAACAAAGCTAATGGTCCTCGACTTTGATGTTGAGGGTGCA TGTGTGAAGTGCTCATAGGAGACGGTGGGGCACTTCCGGTGGAGCCTACGCCGCCGTCTCGCTTGGATTCAGGCGGTGAA CGGTCAGGGACGGGGCAGCTAGTATACAGCTTCAAGCGAGATTTCATTGCAAACTCATCCAAACACAGAAAAGCACACAC AGACAGATGCCGCGGATGTGAGGGTCCGCAACGTAATGTTGAATGCCTTGCCAACAAAACGCAACTGCTCACATACACCA CACACACCATTAACACCACACACACCGTAACACACACACAAAAAACATTAAACAATTAAGGGAAAGAAGAGTAGGGTAGT CGGGTGGTCGGGGTGTGGCTGTGGCTGTGAGTGTATATTGTTAATAATACCCTTTTAAATTACGTTGCTTCATAGCCAAA ATTCGTTTTCCACCGTAGAATAATTTCGCTTCTTAGTTTTTATTGCTATTGTGTATATGGCAAGAATCGATTACCTTAAT GATAATATTATGATTATGATTACGTATTATAAAGCGCAGATTTACAACTGCAGATGCAAATAATTGTTTCGCGTCGTGTG AACCTGAGAGAGCGTTTATGAGAACAGAGCTTTATATTGATTAAATTGTAATTTGTTTAAAAAAAAAAAAAAAAAA
Full-length open reading frame fused to a carboxyl-terminal FLAG-HA tag cassette under the control of a metallothionein promoter.
A cDNA sequence encoding each bait molecule was cloned into the pMK33-C-TAP-FLAG-HA-BD vector as a full-length open reading frame, fused to a carboxyl-terminal FLAG-HA tag cassette under the control of a metallothionein promoter. cDNA sequences are derived from previously characterized and publicly available clones created by the Berkeley Drosophila Genome Project; all inserts are full-length sequence-validated.
