TATAACTTTCACACCTCTACCTTGTCTTGCAGTCAGTATTTAGTCAAACAAAAGAAAAATAAACTACATTTGTTAGAGCT TGATAAGAGCTCAGTTTGTCTTCAGTGTGTGTGCCCGTCATCTAGCAACAAACCGACGTGATTATTTCGACTAAACGTTC CAATGGAAACCGAATCTTAAACCAGAAACCGACTAAACTCGAACGCAATTAAACAACAATAAATCCTCGAAACAAATCAA ATACAAACTGTTACCAAGTGTGCAATTCAAGAAAACATCGTATTGGCATTGTTTTCGCAAAGATACATTTACGTTGTAAA GGTCTGTCAATAACTAGACCTCACTGTAGTAGTAAAGATGACGCAACCACTGCCCATCCGCTTTCAGGAGCATTTACAGC TCACAAATGTCGGGATCAACGCCAATTCATTTTCATTCAGCACGCTCACGATGGAATCGGATAAGTTTATTTGCGTGCGG GAGAAGGTGAATGATACCGCCCAAGTGGTCATCATTGACATGAACGATGCCACCAATCCCACGCGGCGTCCCATCTCAGC GGATTCGGCTATCATGAATCCAGCTAGCAAGGTCATTGCGCTCAAAGCGCAAAAGACGTTGCAGATCTTCAACATTGAGA TGAAGTCGAAGATGAAGGCGCATACCATGAACGAGGATGTGGTGTTCTGGAAGTGGATCTCCCTCAATACGCTAGCTCTG GTCACAGAAACGAGTGTGTTCCATTGGTCCATGGAGGGCGATTCGATGCCGCAAAAGATGTTTGACCGGCATTCATCGCT GAACGGTTGTCAAATCATCAACTACCGCTGCAATGCCTCCCAGCAGTGGCTACTCTTGGTCGGCATTTCGGCACTGCCAA GTCGCGTTGCCGGTGCCATGCAGTTGTATTCGGTGGAGCGTAAGGTGTCCCAGGCGATCGAGGGGCATGCGGCCAGTTTT GCCACGTTTAAAATCGATGCCAACAAGGAGCCGACCACGCTGTTCTGCTTTGCAGTTCGTACGGCCACTGGGGGCAAGCT TCATATTATCGAAGTTGGTGCTCCGCCGAACGGCAATCAGCCGTTTGCCAAGAAGGCTGTCGATGTCTTCTTTCCGCCGG AAGCACAAAATGATTTCCCTGTGGCTATGCAAGTCTCTGCCAAGTACGACACCATCTACTTGATAACCAAGTATGGATAC ATACATCTGTATGACATGGAGACGGCCACGTGCATATACATGAATCGTATATCGGCCGATACGATCTTTGTTACCGCACC GCATGAGGCAAGTGGTGGCATCATTGGCGTCAATCGCAAGGGACAGGTCCTCTCCGTGACCGTCGACGAGGAGCAGATCA TTCCCTACATCAACACCGTTCTGCAGAATCCCGATTTGGCCCTTCGCATGGCCGTGCGCAACAATTTGGCTGGTGCCGAA GATCTCTTTGTGCGAAAGTTCAACAAGCTCTTTACAGCCGGCCAGTATGCTGAAGCGGCTAAAGTTGCTGCCCTGGCACC CAAGGCCATTCTGCGTACGCCACAGACGATCCAGCGTTTCCAACAGGTGCAGACACCAGCTGGCTCCACGACTCCGCCGC TGCTGCAATACTTTGGCATTCTCCTCGACCAGGGCAAGCTGAACAAGTTCGAGTCTCTCGAGCTGTGCCGTCCCGTCTTG CTGCAGGGCAAGAAGCAGCTGTGCGAGAAGTGGCTGAAGGAGGAGAAGTTGGAATGCAGCGAGGAGTTGGGTGATCTGGT CAAGGCCTCCGATCTTACACTTGCCCTGTCCATCTATCTGCGCGCAAATGTGCCCAACAAGGTTATCCAATGCTTTGCTG AGACTGGGCAGTTCCAGAAGATTGTACTCTACGCCAAGAAGGTCAACTATACGCCCGATTACGTGTTCCTGCTGCGCTCC GTGATGCGAAGCAACCCGGAGCAAGGAGCTGGTTTCGCCTCTATGTTGGTGGCCGAGGAAGAGCCACTGGCGGACATCAA TCAGATTGTGGACATCTTCATGGAGCACTCCATGGTGCAGCAGTGCACTGCATTCCTGCTGGACGCCCTCAAGCATAACC GTCCCGCCGAGGGTGCCCTCCAGACGCGCCTGCTGGAAATGAATCTGATGTCTGCTCCGCAGGTGGCCGACGCCATCCTG GGCAATGCTATGTTCACCCACTACGATCGGGCCCACATTGCCCAGCTGTGCGAGAAGGCTGGACTGCTCCAGCGCGCCCT CGAGCACTACACGGATCTGTATGACATTAAGCGGGCCGTTGTGCACACGCACATGCTGAATGCCGAATGGCTGGTCAGTT TCTTTGGCACGCTGTCGGTGGAGGACTCGCTGGAATGTCTGAAGGCAATGCTTACGGCGAATTTGCGCCAGAACTTGCAG ATCTGTGTGCAGATTGCCACCAAGTATCACGAACAGCTGACCAACAAGGCACTGATTGATCTGTTCGAAGGTTTCAAGAG CTACGACGGACTGTTCTACTTCCTGAGCAGCATTGTCAACTTCTCACAGGATCCCGAAGTGCACTTCAAATACATTCAGG CGGCATGCAAGACTAATCAGATTAAGGAGGTGGAGCGAATTTGCCGTGAATCAAACTGCTACAATCCCGAACGGGTGAAG AACTTCTTGAAGGAGGCCAAGCTGACGGATCAGCTACCATTAATTATTGTTTGTGATCGTTTTGATTTCGTGCACGACTT GGTGCTTTACCTGTATCGTAACAATCTGCAGAAGTACATTGAGATCTATGTGCAGAAAGTGAATCCATCCCGCTTGCCAG TGGTAGTGGGTGGTCTTCTTGATGTTGATTGCAGTGAGGATATAATTAAAAATCTAATTCTCGTGGTCAAGGGACAATTC TCAACCGACGAACTGGTCGAGGAGGTCGAGAAGCGCAACCGTCTCAAGCTTCTCCTTCCCTGGCTGGAGTCCCGAGTTCA CGAGGGCTGCGTCGAGCCAGCCACCCACAACGCGTTGGCCAAGATCTACATTGACTCGAACAACAATCCCGAGAGATATC TTAAGGAGAATCAGTACTACGATAGCCGTGTGGTCGGTCGCTACTGCGAGAAGCGGGATCCCCATTTGGCGTGTGTCGCC TACGAGCGTGGATTGTGCGATCGCGAGCTGATCGCCGTTTGTAACGAGAATTCTCTGTTCAAGAGCGAAGCACGCTACTT GGTTGGTCGCCGCGACGCCGAACTCTGGGCCGAGGTCCTTTCGGAGAGCAATCCATACAAGCGCCAGTTGATCGATCAGG TGGTACAGACCGCTTTGTCCGAGACCCAGGATCCCGATGACATCTCTGTAACGGTCAAAGCATTCATGACCGCCGATTTG CCCAATGAGCTGATCGAACTTCTCGAGAAGATTATTCTGGACTCGTCCGTCTTTAGCGACCATCGCAATCTGCAAAACTT GCTCATTCTCACAGCCATCAAGGCTGATCGCACCCGAGTCATGGACTACATTAACCGGCTGGAGAACTACGATGCACCGG ACATCGCGAACATTGCGATCAGTAATCAGTTGTACGAAGAAGCCTTCGCCATCTTCAAGAAGTTCGATGTGAACACATCG GCCATTCAGGTGCTCATCGATCAAGTGAACAACCTGGAGCGGGCTAACGAGTTCGCCGAGCGGTGCAATGAGCCGGCCGT TTGGTCGCAGCTGGCCAAGGCCCAACTGCAGCAGGGTCTGGTCAAGGAGGCTATCGACTCGTACATCAAGGCTGATGATC CGAGCGCTTACGTCGATGTCGTCGATGTGGCCAGCAAGGTGGAGTCTTGGGATGACCTCGTTCGCTATCTGCAAATGGCA CGCAAGAAGGCGCGCGAATCTTACATCGAGAGCGAATTGATCTATGCCTATGCGCGCACTGGACGTCTGGCCGATCTGGA GGAGTTCATTTCGGGTCCCAACCATGCCGATATCCAGAAGATTGGCAACCGTTGCTTCAGCGACGGCATGTACGATGCAG CGAAGCTACTGTACAACAATGTGAGCAACTTTGCCCGTCTGGCCATCACTTTGGTCTACCTGAAGGAGTTCCAAGGGGCC GTGGACTCGGCGCGGAAAGCCAACTCAACGCGCACATGGAAGGAGGTGTGCTTCGCCTGCGTGGACGCCGAGGAGTTTAG GCTAGCTCAGATGTGCGGCCTGCACATTGTGGTGCATGCCGATGAGCTAGAGGATCTGATTAACTACTATCAAAACCGTG GATACTTCGATGAATTGATTGCGCTACTCGAGTCGGCTTTGGGGCTGGAACGTGCGCACATGGGAATGTTCACCGAATTA GCTATACTTTATTCAAAATTCAAACCTTCCAAAATGCGCGAACACTTGGAGCTGTTCTGGTCTCGCGTTAACATTCCAAA AGTTCTGCGTGCCGCTGAATCGGCTCACTTGTGGTCGGAGCTGGTGTTCCTGTACGATAAGTACGAGGAGTACGATAACG CCGTCCTGGCCATGATGGCTCATCCCACGGAGGCGTGGCGCGAGGGGCACTTTAAGGACATTATCACCAAGGTAGCCAAC ATTGAGCTGTACTACAAGGCTATCGAATTCTATTTGGACTTCAAGCCGCTGCTGTTGAACGACATGCTGCTCGTGCTGGC ACCCAGGATGGATCACACTCGTGCTGTTAGTTACTTCTCCAAAACCGGCTATTTGCCACTCGTCAAGCCTTATCTGCGTT CAGTCCAATCTCTCAATAACAAGGCAATCAACGAAGCCCTGAACGGACTCTTAATCGACGAGGAGGACTACCAGGGTCTG CGCAATTCGATCGATGGATTTGATAACTTTGACAACATTGCGTTGGCACAGAAACTCGAAAAGCACGAACTTACCGAATT CCGTAGGATTGCCGCCTACTTGTACAAGGGAAATAATCGCTGGAAACAGAGCGTTGAGCTCTGCAAAAAGGATAAACTCT ACAAGGATGCTATGGAGTACGCCGCCGAATCTTGCAAGCAAGATATTGCCGAAGAGTTGTTGGGTTGGTTCCTAGAACGT GACGCTTACGATTGTTTTGCAGCTTGTCTTTATCAGTGTTACGACTTGCTGCGCCCTGATGTTATCTTGGAGTTGGCCTG GAAACACAAAATCGTTGACTTTGCCATGCCCTATTTGATTCAGGTTCTGCGCGAATACACAACAAAGGTGGACAAACTGG AGTTGAACGAGGCTCAGCGCGAGAAGGAGGACGATTCCACTGAGCACAAAAACATTATTCAGATGGAGCCACAACTGATG ATCACCGCTGGCCCAGCAATGGGCATTCCTCCACAATATGCACAGAATTATCCACCTGGTGCAGCAACGGTAACGGCGGC AGGAGGACGCAACATGGGCTATCCCTACTTGTAGGACTTGCGCCCGATAATGAGATCATCAAAACAACTTTAAAAACAAA TGTATCAGCATTAAAGGAATACCAATAAAATAAGAAAAATAATCTATTAAAAAAAAAAAAAAAAAA
Full-length open reading frame fused to a carboxyl-terminal FLAG-HA tag cassette under the control of a metallothionein promoter.
A cDNA sequence encoding each bait molecule was cloned into the pMK33-C-TAP-FLAG-HA-BD vector as a full-length open reading frame, fused to a carboxyl-terminal FLAG-HA tag cassette under the control of a metallothionein promoter. cDNA sequences are derived from previously characterized and publicly available clones created by the Berkeley Drosophila Genome Project; all inserts are full-length sequence-validated.
