FB2026_02 , released June 18, 2026
Clone: Dmel\CH321-59D11
Some tools are temporarily unavailable. We are completing maintenance and working to restore these tools as soon as possible. Thank you for your patience.
Open Close
General Information
Symbol
Dmel\CH321-59D11
Species
D. melanogaster
Name
CH321-59D11
FlyBase ID
FBcl0749448
Feature type
genomic_clone
Computed gene(s)
 
Collection Status
N/A
Known Problems
none
FlyBase assessment
    Library
    CHORI-321_BAC
    • Pacman genomic clone collection for D. melanogaster, iso-1 strain, adult, 83kb inserts.
    Strain
      Vector
      Tissue Source
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      adult stage
      organism
      (Venken et al., 2009)
      Sequence Data of the Insert
      Full Length Sequence
      Genomic Location
      2R:24,091,212..24,172,190 [+]
      Total bases
      80,979
      NCBI
      Full Length sequence dataSequence Downloader
      Left End Sequence
      Total bases
      640
      NCBI
      FI358629
      Left End sequence data
      GATCGCTCCTCCTCACCCAGGCGTAACAACAGCAGCAGGTCGGTAAAGGATAGGCTGGGCACCAAGATTTCCAATGATAG
AAGCCGTTCGCGAGACAAGTCGAAGGGCAGGCGCCGGGCCGCAAGGAGCTCCGACGACGATGCGAACCGCGGCAGGTCGG
ATCGTCATGGCAGCCGGAAGAGGGACAACAGATCCCGCGACAGGGCGGCGCCTTCAGAGAAGAGGCAGGAGCGTTCGTAC
AAGCGAAGCTCGCCGGAGGACGACAAGCTGAGGCGCCAGAACAAGGAACAGTCCGAATCCAAGCACGGAAAGCATGATCA
AAACAATAGCGACGACTCGGATCGGAGGGCGGCCAAAAACACCAAGTCCAGCGACAGCCGAGTGGTCTCCTCTGTAACAG
CCGTGGTTGCTCCTCCCAAACCCTGTCGTCCAGACAACCCGTTCCGCAAGTTCGTCGACACCAGTTCGTCGAGCAGCTTA
GTTGTAAAATATGATAACACGATACAGAAGGAGGGCGCGTCCTCGGACAACGGCATGGAGCACAGGAAGCAGAGGGATAA
GAAGCTGAAGAAACATTCAAAATATTCGTCAACCGATTCGTTGAAGAGCGAGAAGCGCAAGGATCCGAAGAGCAAAAAGA
      Right End Sequence
      Total bases
      669
      NCBI
      FI358630
      Right End sequence data
      GATCCACTCCAGACTCCGCTGATTGGCAGACCGCCTGCCCAGATTGGACTCCCGCAAGCCCGACTCCTGGTACTCGAATC
CGAACACCTGGGTGATGGGATTGTCCACGGACTCGTTGGTGTTGCTCAGGTTCTGTAGGTCCAGAGCATCGGGATTGTCG
CTGCTGTTTCTCACGGGAAGAGCCGGGGGCGGCACATCCAGAGCGTGTGTTAGTGGACTCTGGGCGGGCTCAGTGCTCAC
CTTGTTGGTGGTCAGAGCCAGATTGGCCGTTGGGGGCGATACGGTCTTGTAAACGTGGGTGTAGACGGTTCCAGGATTGA
ATTTGCGAATGGGATGAGCGGCGACGGATGGTGAGTTGGAATCGTAGGCATCGGAGGCGGGACTGGCGATGGTCCCCACC
ACCACGATGTGCTCGTCCAGCTTCAGCTGGGACTGCTTCTGCTGGGCATCGATGTCTATGGAGTTGTTCTCGAAGTCCTG
AGGCAGAGGAATGGCCTGGGGTGGCTTCTCCGTGGAGGCCACTGTGTCGTTCAAGGCAGCTGGAGGAACCTTCGCTGGAG
CAGCGGGAACCACATGTACCGGCTGAGGTAGGTGCAGTTTCTGACCCGCCGCCTGACGCAGCACCTCCTGGGTGGGCGGC
CACTTGCCCTTTTTGCTGCTGATGGTCTT
      Library Information (1)
      Library: CHORI-321_BAC
      Description

      A BAC library containing genomic DNA fragments (average size of 83 kb) in the attB-P[acman]-CmR-BW vector for efficient transgenesis.

      (Venken et al., 2009)
      Sample preparation
      Protocol

      DNA isolated from adult fly tissue was prepared in agarose blocks and partially digested with MboI. 80-100 kb fragments were size-selected by contour-clamped homogeneous electric field (CHEF) gel electrophoresis, and ligated into the BamHi site of the attB-P[acman]-CmR-BW vector, a special adaptation of the P[acman] vector. Libraries were transformed into the E.coli strain "EPI300-T1R".

      (Venken et al., 2009)
      Comment

      A total of 36,864 clones were generated.

      (Venken et al., 2009)
      Reported As
      Symbol Synonyms
      CHORI-321
      (Venken et al., 2009)
      CHORI-321_BAC
      References (3)
      Research paper (2)
      Venken et al., 2010, Genetics 186(4): 1111--1125
      A molecularly defined duplication set for the X chromosome of Drosophila melanogaster. [FBrf0212670]
      Venken et al., 2009, Nat. Methods 6(6): 431--434
      Versatile P[acman] BAC libraries for transgenesis studies in Drosophila melanogaster. [FBrf0209231]
      Personal communication to FlyBase (1)
      GenetiVision Corporation, 2016-, A collection of transgenic flies carrying duplications that tile the autosomal portion of the Drosophila melanogaster genome.
      A collection of transgenic flies carrying duplications that tile the autosomal portion of the Drosophila melanogaster genome. [FBrf0232631]
      External Crossreferences and Linkouts ( 1 )
      Linkouts
      PACMANFLY - The source for information and resources of tools for the Drosophila community using the P[acman] recombineering technique.
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      CH321-59D11
      Secondary FlyBase IDs
        References (1)