The R89B01 enhancer fragment is fused upstream of the Drosophila synthetic core promoter (DSCP) followed by the GAL4(DBD)::Zip- hemidriver (component of the 'Split GAL4' system, contains the GAL4 DNA-binding domain). A Hsp70 transcriptional terminator is present.
The R13F02 enhancer fragment is fused upstream of the Drosophila synthetic core promoter (DSCP) followed by the p65(AD)::Zip+ hemidriver (component of a 'split driver' system, contains the p65 activation domain). A Hsp70 transcriptional terminator is present.
In a split-GAL4 system, ScerGAL4DBD.R89B01 drives expression in the gamma main Kenyon cells and, stochastically, in the gamma dorsal Kenyon cells when combined with HsapRELAAD.R13F02.
In a split-GAL4 system, HsapRELAAD.R13F02 drives expression in the gamma dorsal Kenyon cells, gamma main Kenyon cells, alpha'/beta' anterior-posterior Kenyon cells, alpha'/beta' middle Kenyon cells, alpha/beta posterior Kenyon cells, alpha/beta surface Kenyon cells and alpha/beta core Kenyon cells when combined with ScerGAL4DBD.R52H09, in the gamma dorsal Kenyon cells, gamma main Kenyon cells, alpha/beta posterior Kenyon cells, alpha/beta surface Kenyon cells and alpha/beta core Kenyon cells when combined with ScerGAL4DBD.R21B06, in the the gamma dorsal Kenyon cells and gamma main Kenyon cells when combined with HsapRELAAD.R13F02, in the alpha/beta posterior Kenyon cells, alpha/beta surface Kenyon cells and alpha/beta core Kenyon cells when combined with ScerGAL4DBD.R44E04, in the gamma main Kenyon cells and, stochastically, in the gamma dorsal Kenyon cells when combined with ScerGAL4DBD.R89B01, in the alpha/beta posterior Kenyon cells when combined with ScerGAL4DBD.R85D07, and in the alpha'/beta' anterior-posterior Kenyon cells and alpha'/beta' middle Kenyon cells when combined with ScerGAL4DBD.R34A03 or ScerGAL4DBD.R41C07.
Labelling of gamma dorsal Kenyon cells is stochastic.