FB2026_03 , released September 17, 2026
Split System Combination: Scer\GAL4DBD.R49F03∩Hsap\RELAAD.R52G04
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General Information
Symbol
Scer\GAL4DBD.R49F03∩Hsap\RELAAD.R52G04
FlyBase ID
FBco0000083
Feature Type
split system combination
Component Alleles
Combination components
Component allele Hsap\RELAAD.R52G04
Component
Use(s)
Regulatory region(s)
GMR52G04
Encoded product / tool
p65(AD)::Zip+
Description

The R52G04 enhancer fragment is fused upstream of the Drosophila synthetic core promoter (DSCP) followed by the p65(AD)::Zip+ hemidriver (component of a 'split driver' system, contains the p65 activation domain). A Hsp70 transcriptional terminator is present.

(Aso et al., 2014)
Component allele Scer\GAL4DBD.R49F03
Component
Use(s)
Regulatory region(s)
GMR49F03
Encoded product / tool
GAL4(DBD)::Zip-
Description

The GMR49F03 enhancer fragment followed by the Drosophila synthetic core promoter (DSCP) is fused upstream of the GAL4(DBD)::Zip- hemidriver (component of the 'Split GAL4' system, contains the GAL4 DNA-binding domain).

(Rubin Laboratory at Janelia Farm, 2017)
Expression Data
Reporters Expression
distribution deduced from reporter (Gal4 UAS)
Stage
Tissue/Position (including subcellular localization)
Reference
adult stage
mushroom body output neuron 7
(Vogt et al., 2016)
medulla columnar neuron VPN-MB2 | subset
(Vogt et al., 2016)
Additional Information
Statement
Reference

In a split-GAL4 system, HsapRELAAD.R52G04 drives expression in 1-3 medulla columnar neuron VPN-MB2 as well as mushroom body output neuron 7 when combined with ScerGAL4DBD.R49F03 and in mushroom body output neuron 7 when combined with ScerGAL4DBD.R17C11. Expression overlaps with that driven by EcollexAGMR13F02 in the Kenyon cells. Positive GRASP (GFP reconstitution across synaptic partners) signals were seen between medulla columnar neuron VPN-MB2 and gamma dorsal Kenyon cells using these split-GAL4 drivers and AvicGFPspGFP11.Mef2.mb247.T:HsapCD4.

(Vogt et al., 2016)

In a split-GAL4 system, ScerGAL4DBD.R49F03 drives expression in 1-3 medulla columnar neuron VPN-MB2 as well as mushroom body output neuron 7 when combined with HsapRELAAD.R52G04. Expression overlaps with that driven by EcollexAGMR13F02 in the Kenyon cells. Positive GRASP (GFP reconstitution across synaptic partners) signals were seen between medulla columnar neuron VPN-MB2 and gamma dorsal Kenyon cells using these split-GAL4 drivers and AvicGFPspGFP11.Mef2.mb247.T:HsapCD4.

(Vogt et al., 2016)

Labels 1-3 medulla columnar neuron VPN-MB2 neurons.

(Vogt et al., 2016)
Marker for
Stocks (0)
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
MB334C
(Vogt et al., 2016)
Scer\GAL4DBD.R49F03∩Hsap\RELAAD.R52G04
Name Synonyms
Secondary FlyBase IDs
    References (1)