The R59C10 enhancer fragment and Drosophila synthetic core promoter (DSCP) drive expression of the GAL4(DBD)::Zip- hemidriver (component of the 'Split GAL4' system, contains the GAL4 DNA-binding domain). A Hsp70 transcriptional terminator is also present.
The GMR38C11 enhancer fragment followed by the Drosophila synthetic core promoter (DSCP) is fused upstream of the p65(AD)::Zip+ hemidriver (component of a 'split driver' system, contains the p65 activation domain).
In a split-GAL4 system, ScerGAL4DBD.R59C10 drives expression in transmedullary neuron Tm3a when combined with HsapRELAAD.R38C11.