The R17A04 enhancer fragment is fused upstream of the Drosophila synthetic core promoter (DSCP) followed by the p65(AD)::Zip+ hemidriver (component of a 'split driver' system, contains the p65 activation domain). A Hsp70 transcriptional terminator is present.
The R35B06 enhancer fragment is fused upstream of the Drosophila synthetic core promoter (DSCP) followed by the GAL4(DBD)::Zip- hemidriver (component of the 'Split GAL4' system, contains the GAL4 DNA-binding domain). A Hsp70 transcriptional terminator is present.
In a split-GAL4 system, HsapRELAAD.R17A04 drives expression in lobula columnar neuron LC20 and a subset of neurons in the thoracico-abdominal ganglion when combined with ScerGAL4DBD.R35B06.
In a split-GAL4 system, ScerGAL4DBD.R35B06 drives expression in lobula columnar neuron LC20 and a subset of neurons in the thoracico-abdominal ganglion when combined with HsapRELAAD.R17A04, lobula columnar neuron LC20 when combined with HsapRELAAD.R71F06, lobula columnar neuron LC21 when combined with HsapRELAAD.R50C03, and lobula columnar neuron LC22 when combined with HsapRELAAD.R64G10.