The R91B01 enhancer fragment is fused upstream of the Drosophila synthetic core promoter (DSCP) followed by the p65(AD)::Zip+ hemidriver (component of a 'split driver' system, contains the p65 activation domain). A Hsp70 transcriptional terminator is present.
The R22A07 enhancer fragment is fused upstream of the Drosophila synthetic core promoter (DSCP) followed by the GAL4(DBD)::Zip- hemidriver (component of the 'Split GAL4' system, contains the GAL4 DNA-binding domain). A Hsp70 transcriptional terminator is present.
In a split-GAL4 system, HsapRELAAD.R91B01 drives expression in lobula columnar neuron Lcn6 when combined with ScerGAL4DBD.R41C07, or Lcn6 and a subset of neurons in the thoracico-abdominal ganglion when combined with ScerGAL4DBD.R22A07.
In a split-GAL4 system, ScerGAL4DBD.R22A07 drives expression in lobula columnar neuron Lcn6 and a subset of neurons in the thoracico-abdominal ganglion when combined with HsapRELAAD.R91B01, and lobula columnar neuron LC10b, LC10c and a single LC10a neuron when combined with HsapRELAAD.R35D04.