The GMR28F07 enhancer fragment followed by the Drosophila synthetic core promoter (DSCP) is fused upstream of the p65(AD)::Zip+ hemidriver (component of a 'split driver' system, contains the p65 activation domain).
The GMR10E05 enhancer fragment followed by the Drosophila synthetic core promoter (DSCP) is fused upstream of the GAL4(DBD)::Zip- hemidriver (component of the 'Split GAL4' system, contains the GAL4 DNA-binding domain).
In a split-GAL4 system, HsapRELAAD.R28F07 drives expression in medulla columnar neuron MC63 when combined with ScerGAL4DBD.R10E05. Expression overlaps with that driven by EcollexAGMR13F02 in the Kenyon cells. Positive GRASP (GFP reconstitution across synaptic partners) signals were seen between medulla columnar neuron MC63 and gamma dorsal Kenyon cells using these split-GAL4 drivers and AvicGFPspGFP11.Mef2.mb247.T:HsapCD4.
