FlyBase Split System Combination Report: Scer\GAL4[DBD.VT049344]∩Hsap\RELA[AD.R47E07]

General Information

Symbol

Scer\GAL4^DBD.VT049344∩Hsap\RELA^AD.R47E07

FlyBase ID

FBco0000308

Feature Type

split system combination

Component Alleles

Hsap\RELA^AD.R47E07

Scer\GAL4^DBD.VT049344

Combination components

Component allele Hsap\RELA^AD.R47E07

Component

Use(s)

Regulatory region(s)

GMR47E07

Encoded product / tool

p65(AD)::Zip+

split driver - transcription activation fragment

Description

The R47E07 enhancer fragment is fused upstream of the Drosophila synthetic core promoter (DSCP) followed by the p65(AD)::Zip+ hemidriver (component of a 'split driver' system, contains the p65 activation domain). A Hsp70 transcriptional terminator is present.

(Wu et al., 2016)

Component allele Scer\GAL4^DBD.VT049344

Component

Use(s)

Regulatory region(s)

VT049344

Encoded product / tool

GAL4(DBD)::Zip-

split driver - DNA-binding fragment

Description

The VT049344 enhancer fragment is fused upstream of the Drosophila synthetic core promoter (DSCP) followed by the GAL4(DBD)::Zip- hemidriver (component of the 'Split GAL4' system, contains the GAL4 DNA-binding domain).

(Wu et al., 2016)

Expression Data

Reporters Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

lobula columnar neuron LC6

(Wu et al., 2016)

adult ventral nerve cord | restricted

(Wu et al., 2016)

Additional Information

Statement

Reference

In a split-GAL4 system, HsapRELA^AD.R47E07, inserted in P{R47E07-p65.AD}, drives expression in lobula columnar neuron Lcn6 when combined with ScerGAL4^DBD.R41C07, Lcn6 and a subset of neurons in the thoracico-abdominal ganglion when combined with ScerGAL4^DBD.VT049344, lobula columnar neuron LC10b and lobula columnar neuron LC10d when combined with ScerGAL4^DBD.R35D04 and, when inserted in PBac{R47E07-p65.AD}, lobula columnar neuron LC10c when combined with ScerGAL4^DBD.R35D04.

(Wu et al., 2016)

In a split-GAL4 system, ScerGAL4^DBD.VT049344 drives expression in lobula columnar neuron Lcn6 when combined with HsapRELA^AD.R47E07.

(Wu et al., 2016)

Marker for

Stocks (1)

Bloomington

68380

w¹¹¹⁸; P{R47E07-p65.AD}attP40; P{VT049344-GAL4.DBD}attP2

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym