The R85H06 enhancer fragment is fused upstream of the Drosophila synthetic core promoter (DSCP) followed by the GAL4(DBD)::Zip- hemidriver (component of the 'Split GAL4' system, contains the GAL4 DNA-binding domain). A Hsp70 transcriptional terminator is present.
The GMR20C05 enhancer fragment followed by the Drosophila synthetic core promoter (DSCP) is fused upstream of the p65(AD)::Zip+ hemidriver (component of a 'split driver' system, contains the p65 activation domain).
ScerGAL4DBD.R85H06 expression when combined with HsapRELAAD.R66B05 is stochastic in descending neuron of the gnathal ganglion DNg11 and when combined with HsapRELAAD.R20C05 is stochastic in descending neuron of the posterior brain DNp20.
HsapRELAAD.R20C05 expression when combined with ScerGAL4DBD.R85H06 is stochastic in descending neuron of the posterior brain DNp20.
Labelling of descending neuron of the posterior brain DNp20 is stochastic.