The R35D04 enhancer fragment is fused upstream of the Drosophila synthetic core promoter (DSCP) followed by the p65(AD)::Zip+ hemidriver (component of a 'split driver' system, contains the p65 activation domain). A Hsp70 transcriptional terminator is present.
The VT043656 enhancer fragment is fused upstream of the Drosophila synthetic core promoter (DSCP) followed by the GAL4(DBD)::Zip- hemidriver (component of the 'Split GAL4' system, contains the GAL4 DNA-binding domain).
HsapRELAAD.R35D04 labels approximately 100 cells per hemisphere - mostly LC10a with a small number of LC10b neurons - when in combination with ScerGAL4DBD.VT043656.
ScerGAL4DBD.VT043656 labels approximately 100 cells per hemisphere - mostly LC10a with a small number of LC10b neurons - when in combination with HsapRELAAD.R35D04 and HsapRELAAD.R25H09, and approximately 100 LC10a neurons per hemisphere when in combination with HsapRELAAD.VT040012, approximately 60% of which are fruitless-expressing lobula columnar neuron LC10a that EcollexAfru.P1.
Labels approximately 100 cells per hemisphere - mostly LC10a with a small number of LC10b neurons.
In a split-GAL4 system, HsapRELAAD.R35D04 drives expression in lobula columnar neuron LC10a when combined with ScerGAL4DBD.R22D06, lobula columnar neuron LC10b when combined with ScerGAL4DBD.VT043656, lobula columnar neuron LC10b, LC10c and a single LC10a neuron when combined with ScerGAL4DBD.R22A07, lobula columnar neuron LC10a and LC10d when combined with ScerGAL4DBD.R80E07 or ScerGAL4DBD.R91B01, lobula columnar neuron LC10b and LC10c when combined with ScerGAL4DBD.VT040747, lobula columnar neuron LC10a, LC10b and LC10c when combined with ScerGAL4DBD.R82D11, lobula columnar neuron LC10a, LC10b and LC10c and a subset of neurons in the thoracico-abdominal ganglion when combined with ScerGAL4DBD.VT058944, lobula columnar neuron LC10a, LC10b, LC10c and LC10d when combined with ScerGAL4DBD.R71E06, lobula columnar neuron LC10a, LC10d and a single neuron each of LC10b and LC10C when combined with ScerGAL4DBD.VT045997, lobula columnar neuron LC12 when combined with ScerGAL4DBD.R65B05 or LC12 and a subset of neurons in the thoracico-abdominal ganglion when combined with ScerGAL4DBD.R55F01.
In a split-GAL4 system, ScerGAL4DBD.VT043656 drives expression in lobula columnar neuron LC10b when combined with HsapRELAAD.R35D04.