Open Close
General Information
Symbol
Scer\GAL4DBD.VT043656
Species
S. cerevisiae
Name
FlyBase ID
FBal0326471
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

The VT043656 enhancer fragment is fused upstream of the Drosophila synthetic core promoter (DSCP) followed by the GAL4(DBD)::Zip- hemidriver (component of the 'Split GAL4' system, contains the GAL4 DNA-binding domain).

Allele components
Product class / Tool use(s)
Associated Sequence Features
Expression Data
Reporter Expression
distribution deduced from reporter (Gal4 UAS)
Stage
Tissue/Position (including subcellular localization)
Reference
lobula columnar neuron LC10a

Comment: when combined with P{R25H09-p65.AD} (combination referred to as 'SS3')

when combined with P{R35D04-p65.AD} (combination referred to as 'SS2')

when combined with P{VT040012-p65.AD} (combination referred to as 'SS1')

lobula columnar neuron LC10b | subset

Comment: when combined with P{R25H09-p65.AD} (combination referred to as 'SS3')

when combined with P{R35D04-p65.AD} (combination referred to as 'SS2')

lobula columnar neuron LC10b

Comment: when combined with P{R35D04-p65.AD} (combination referred to as 'SS02664')

Additional Information
Statement
Reference

Scer\GAL4DBD.VT043656 labels approximately 100 cells per hemisphere - mostly LC10a with a small number of LC10b neurons - when in combination with Hsap\RELAAD.R35D04 and Hsap\RELAAD.R25H09, and approximately 100 LC10a neurons per hemisphere when in combination with Hsap\RELAAD.VT040012, approximately 60% of which are fruitless-expressing lobula columnar neuron LC10a that Ecol\lexAfru.P1.

In a split-GAL4 system, Scer\GAL4DBD.VT043656 drives expression in lobula columnar neuron LC10b when combined with Hsap\RELAAD.R35D04.

 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Inhibition (using Ctet\tetXTNT-LC.UAS) of neurons targeted by the split-GAL4 pairs of Scer\GAL4DBD.VT043656 and Hsap\RELAAD.VT040012, or Scer\GAL4DBD.VT043656 and Hsap\RELAAD.R35D04, or Scer\GAL4DBD.VT043656 and Hsap\RELAAD.R25H09, reduces the ability of males to orient towards and remain close to a female, compared to controls; they also lose the bias seen in controls to extend the wing closest to, rather than furthest from, the female; following of females, wing extensions and copulation are significantly reduced, compared to controls.

Inhibition (using Ctet\tetXTNT-LC.UAS) of neurons targeted by the split-GAL4 pairs of Scer\GAL4DBD.VT043656 and Hsap\RELAAD.VT040012, or Scer\GAL4DBD.VT043656 and Hsap\RELAAD.R35D04, in adult males does not reduce courtship of decapitated (immobile) females, compared to controls; this inhibition does not affect the ability of adult males to track a rotating grating or a bar, but they are less able to track small fly-sized objects, with no effect on the overall distribution of turning and walking speeds and no effect on movement in the absence of motion stimuli, compared to controls.

Feminization (using traUAS.cFa) of neurons targeted by the split-GAL4 pairs of Scer\GAL4DBD.VT043656 and Hsap\RELAAD.VT040012, or Scer\GAL4DBD.VT043656 and Hsap\RELAAD.R35D04, in adult males does not affect tracking or courtship of females, compared to controls.

Optogenetic activation (using Cnoc\ChR1::Csub\ChRCsChrimson.20xUAS.FRT.Venus with Scer\FLP1G5.hs.Tag:PEST(mOdc) and 627 nm (red) light) of unilateral subsets of neurons targeted by the split-GAL4 pair of Scer\GAL4DBD.VT043656 and Hsap\RELAAD.VT040012 in adult males results in increased ipsilateral turning and significantly increased ipsilateral wing extensions, compared to controls, with no effect on walking speed.

Unilateral optogenetic activation (using Cnoc\ChR1::Csub\ChRCsChrimson.IVS.20xUAS.Venus with 627 nm (red) light) of neurons targeted by the split-GAL4 pair of Scer\GAL4DBD.VT043656 and Hsap\RELAAD.VT040012 in adult males results in increased ipsilateral turning, compared to controls.

Optogenetic activation (using Cnoc\ChR1::Csub\ChRCsChrimson.IVS.20xUAS.Venus with 627 nm (red) light) of neurons targeted by the split-GAL4 pair of Scer\GAL4DBD.VT043656 and Hsap\RELAAD.R35D04 in adult males results in significantly increased wing extensions, compared to controls, with no increase in turning.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
Comments
Comments

Split-GAL4 transgene, constructed as described in FBrf0212052.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
Scer\GAL4DBD.VT043656.T:Zzzz\Zip-
Scer\GAL4DBD.VT043656
Name Synonyms
Secondary FlyBase IDs
    References (5)