The VT043920 enhancer fragment is fused upstream of the Drosophila synthetic core promoter (DSCP) followed by the p65(AD)::Zip+ hemidriver (component of a 'split driver' system, contains the p65 activation domain).
The R91B01 enhancer fragment is fused upstream of the Drosophila synthetic core promoter (DSCP) followed by the GAL4(DBD)::Zip- hemidriver (component of the 'Split GAL4' system, contains the GAL4 DNA-binding domain). A Hsp70 transcriptional terminator is present.
In a split-GAL4 system, HsapRELAAD.VT043920 drives expression in lobula columnar neuron LC10d when combined with ScerGAL4DBD.R91B01.
In a split-GAL4 system, ScerGAL4DBD.R91B01 drives expression in lobula columnar neuron LC10d when combined with HsapRELAAD.VT043920, lobula columnar neuron LC10a and lobula columnar neuron LC10d when combined with HsapRELAAD.R35D04, and lobula columnar neuron LC15 when combined with HsapRELAAD.R24A02.