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General Information
Symbol
Scer\GAL4DBD.R91B01
Species
S. cerevisiae
Name
FlyBase ID
FBal0326462
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

The R91B01 enhancer fragment is fused upstream of the Drosophila synthetic core promoter (DSCP) followed by the GAL4(DBD)::Zip- hemidriver (component of the 'Split GAL4' system, contains the GAL4 DNA-binding domain). A Hsp70 transcriptional terminator is present.

Allele components
Product class / Tool use(s)
Associated Sequence Features
Expression Data
Reporter Expression
distribution deduced from reporter (Gal4 UAS)
Stage
Tissue/Position (including subcellular localization)
Reference
lobula columnar neuron LC15

Comment: when combined with P{R24A02-p65.AD} (combination referred to as 'OL0004B')

lobula columnar neuron LC10a

Comment: when combined with P{R35D04-p65.AD} (combination referred to as 'OL0022B')

lobula columnar neuron LC10d

Comment: when combined with P{R35D04-p65.AD} (combination referred to as 'OL0022B')

when combined with P{VT043920-p65.AD} (combination referred to as 'SS03822')

Additional Information
Statement
Reference

In a split-GAL4 system, Scer\GAL4DBD.R91B01 drives expression in lobula columnar neuron LC10d when combined with Hsap\RELAAD.VT043920, lobula columnar neuron LC10a and lobula columnar neuron LC10d when combined with Hsap\RELAAD.R35D04, and lobula columnar neuron LC15 when combined with Hsap\RELAAD.R24A02.

 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Inhibition (using Ctet\tetXTNT-LC.UAS) of neurons targeted by the split-GAL4 pair of Scer\GAL4DBD.R91B01 and Hsap\RELAAD.VT043920, or Scer\GAL4DBD.R91B01 and Hsap\RELAAD.R35D04, in adult males does not affect their ability to orient towards and remain close to a female, compared to controls; in the case of Scer\GAL4DBD.R91B01 and Hsap\RELAAD.R35D04, but not Scer\GAL4DBD.R91B01 and Hsap\RELAAD.VT043920, wing extensions are significantly reduced, compared to controls, with no effect on following of females.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (3)
Notes on Origin
Discoverer
Comments
Comments

Split-GAL4 transgene, constructed as described in FBrf0212052.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
Scer\GAL4DBD.R91B01.T:Zzzz\Zip-
Scer\GAL4DBD.R91B01
Name Synonyms
Secondary FlyBase IDs
    References (4)