FlyBase Split System Combination Report: Scer\GAL4[DBD.Mef2.247]∩Hsap\RELA[AD.12xCRE]

General Information

Symbol

Scer\GAL4^DBD.Mef2.247∩Hsap\RELA^AD.12xCRE

FlyBase ID

FBco0001691

Feature Type

split system combination

Component Alleles

Scer\GAL4^DBD.Mef2.247

Hsap\RELA^AD.12xCRE

Combination components

Component allele Scer\GAL4^DBD.Mef2.247

Component

Use(s)

Regulatory region(s)

Mef2

Encoded product / tool

GAL4(DBD)::Zip-

split driver - DNA-binding fragment

Description

A 247 bp sequence of Mef2 encoding the MB247 enhancer and the hsp70 core promoter regulate expression of a GAL4(DBD)::Zip- hemidriver (component of the 'Split GAL4' system, contains the GAL4 DNA-binding domain).

(Yamazaki et al., 2018)

Component allele Hsap\RELA^AD.12xCRE

Component

Use(s)

Encoded product / tool

p65(AD)::Zip+

split driver - transcription activation fragment

Description

p65(AD)::Zip+ is under the control of 12 cAMP response element (CRE, TGACGTCA) repeats. A yeast-derived degron (CL1) is attached, to accelerate protein turnover and avoid toxicity.

(Yamazaki et al., 2018)

Expression Data

Reporters Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

alpha'/beta' Kenyon cell | subset

(Yamazaki et al., 2018)

alpha/beta Kenyon cell | subset

(Yamazaki et al., 2018)