The GMR70H02 enhancer fragment followed by the Drosophila synthetic core promoter (DSCP) is fused upstream of the p65(AD)::Zip+ hemidriver (component of a 'split driver' system, contains the p65 activation domain).
The GMR76F12 enhancer fragment followed by the Drosophila synthetic core promoter (DSCP) is fused upstream of the GAL4(DBD)::Zip- hemidriver (component of the 'Split GAL4' system, contains the GAL4 DNA-binding domain).
In a split-GAL4 system, HsapRELAAD.R70H02 drives expression in antennal grooming descending neuron 2, the prothoracic, mesothoracic and metathoracic neuromeres, dorsolateral part of the brain and ascending neurons in the thoracico-abdominal ganglion when combined with ScerGAL4DBD.R18C11, and in antennal grooming descending neuron 2, the adult pars intercerebralis, the lateral part of the brain and in the thoracico-abdominal ganglion when combined with ScerGAL4DBD.R76F12.
In a split-GAL4 system, ScerGAL4DBD.R76F12 drives expression in antennal grooming descending neuron 2, the adult pars intercerebralis, the lateral part of the brain and in the thoracico-abdominal ganglion when combined with HsapRELAAD.R70H02.