FlyBase Split System Combination Report: Scer\GAL4[DBD.R29G11]∩Hsap\RELA[AD.R53G02]

General Information

Symbol

Scer\GAL4^DBD.R29G11∩Hsap\RELA^AD.R53G02

FlyBase ID

FBco0001773

Feature Type

split system combination

Component Alleles

Scer\GAL4^DBD.R29G11

Hsap\RELA^AD.R53G02

Combination components

Component allele Scer\GAL4^DBD.R29G11

Component

Use(s)

Regulatory region(s)

GMR29G11

Encoded product / tool

GAL4(DBD)::Zip-

split driver - DNA-binding fragment

Description

The R29G11 enhancer fragment is fused upstream of the Drosophila synthetic core promoter (DSCP) followed by the GAL4(DBD)::Zip- hemidriver (component of the 'Split GAL4' system, contains the GAL4 DNA-binding domain). An Hsp70 transcriptional terminator is present.

(Chen et al., 2014)

Component allele Hsap\RELA^AD.R53G02

Component

Use(s)

Regulatory region(s)

GMR53G02

Encoded product / tool

p65(AD)::Zip+

split driver - transcription activation fragment

Description

The R53G02 enhancer fragment is fused upstream of the Drosophila synthetic core promoter (DSCP) followed by the p65(AD)::Zip+ hemidriver (component of a 'split driver' system, contains the p65 activation domain). A Hsp70 transcriptional terminator is present.

(Tuthill et al., 2013)

Expression Data

Reporters Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

lamina monopolar neuron L2

(Lillvis et al., 2022, Kind et al., 2021, Drews et al., 2020, Tuthill et al., 2013)

lamina monopolar neuron L2 | subset

(Yuan et al., 2021)

Additional Information

Statement

Reference

Employing the intersectional Split-Gal4 method ScerGAL4^DBD.R29G11 drives expression in a subset of lamina monopolar neurons L1 and L2 when combined with HsapRELA^AD.R75H09 or HsapRELA^AD.R48A08. In combination with HsapRELA^AD.R53G02 expression is exclusively seen in lamina monopolar neuron L2. In combination with HsapRELA^AD.R35A03 expression is driven in centrifugal neurons C3 and in less than 20 lobula plate intrinsic neurons. In combination with HsapRELA^AD.R26H02 expression is driven exclusively in centrifugal neurons C3.

(Tuthill et al., 2013)

Marker for

lamina monopolar neuron L2

(Tuthill et al., 2013)

Stocks (1)

Bloomington

86946

w¹¹¹⁸; P{R53G02-p65.AD}attP40/CyO; P{R29G11-GAL4.DBD}attP2

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym