The GMR31H10 enhancer fragment followed by the Drosophila synthetic core promoter (DSCP) is fused upstream of the p65(AD)::Zip+ hemidriver (component of a 'split driver' system, contains the p65 activation domain).
The GMR34E03 enhancer fragment followed by the Drosophila synthetic core promoter (DSCP) is fused upstream of the GAL4(DBD)::Zip- hemidriver (component of the 'Split GAL4' system, contains the GAL4 DNA-binding domain).
In a split-GAL4 system, HsapRELAAD.R31H10 drives expression in sensillum campaniformium neurons in the proximal part of the wing, campaniformium neurons in the distal part of the wing, sensillum campaniformium neurons in the halteres and mechanosensory neurons that are part of the wing margin bristle when combined with ScerGAL4DBD.R34E03. Neurons on the wings and halteres send projections to the thoracico-abdominal ganglion, where some ascend to the adult subesophageal zone.