2A-GAL4(DBD)::Zip- is introduced into the attP site in the TI{TI}unc-4FRT progenitor. The Trojan insertion introduces a stop codon, truncating the unc-4 protein after its first exon.
The RMCE cassette of the Mi{MIC}TfAP-2MI04611 insertion has been replaced with a Trojan-p65AD cassette.