The VT026999 enhancer fragment followed by the Drosophila synthetic core promoter (DSCP) is fused upstream of the p65(AD)::Zip+ hemidriver (component of a 'split driver' system, contains the p65 activation domain).

A sequence cassette that encodes the T2A peptide plus the GAL4(DBD)::Zip- hemidriver has been inserted into the endogenous NPF locus, allowing the separate expression of the hemidriver under the control of endogenous NPF regulatory sequences. A loxP cassette encoding a Disc\RFP^3xP3.cUa marker has also been inserted during the mutagenesis event, downstream of the split driver sequence.