FlyBase Split System Combination Report: Scer\GAL4[dsx-GAL4(DBD)::Zip-]∩Hsap\RELA[AD.VT059780]

General Information

Symbol

Scer\GAL4^{dsx-GAL4(DBD)::Zip-}∩Hsap\RELA^AD.VT059780

FlyBase ID

FBco0002400

Feature Type

split system combination

Component Alleles

Hsap\RELA^AD.VT059780

Scer\GAL4^{dsx-GAL4(DBD)::Zip-}

Combination components

Component allele Hsap\RELA^AD.VT059780

Component

Use(s)

Regulatory region(s)

VT059780

Encoded product / tool

p65(AD)::Zip+

split driver - transcription activation fragment

Description

The VT059780 enhancer fragment followed by the Drosophila synthetic core promoter (DSCP) is fused upstream of the p65(AD)::Zip+ hemidriver (component of a 'split driver' system, contains the p65 activation domain).

(Rubin Laboratory at Janelia Farm, 2017)

Component allele Scer\GAL4^{dsx-GAL4(DBD)::Zip-}

Component

Use(s)

Inserted element

TI{GAL4(DBD)::Zip-}

promoter trap

Encoded product / tool

GAL4(DBD)::Zip-

split driver - DNA-binding fragment

Description

A GAL4(DBD)::Zip- hemidriver (component of the 'Split GAL4' system, contains the GAL4 DNA-binding domain) has been inserted at the start codon within exon 2 of dsx. This results in the hemidriver being expressed under the control of endogenous dsx sequences, along with deletion of exon 2.

(Shirangi et al., 2016)

Expression Data

Reporters Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult doublesex TN1C (male) neuron

(Lillvis et al., 2024)

neuron | subset of adult brain

(Lillvis et al., 2024)