CG5321 and CG14630 are likely to be mitochondrial based on MitoFates and TargetP mitochondrial targeting sequence (MTS) prediction and have been detected in the mitochondrion in a high-throughput proteomics study (FBrf0224590). CG10814 has no predicted MTS or experimental data supporting mitochondrial localization. FBrf0233151 suggests that CG10814 is the functional ortholog of human BBOX1 (gamma-butyrobetaine hydroxylase 1; HGNC:964), which acts in the cytosol in the carnitine biosynthesis pathway, due to its lack of a MTS (FBrf0247638).