FlyBase Gene Report: Dmel\Lap-D

General Information

Symbol

Dmel\Lap-D

Species

D. melanogaster

Name

Leucine aminopeptidase D

Annotation Symbol

Feature Type

gene

FlyBase ID

FBgn0002530

Gene Model Status

Unannotated

Stock Availability

2 publicly available

Gene Summary

Contribute a Gene Snapshot for this gene.

All Summaries

Alliance
Auto summary
Red Book
Interactive Fly

Also Known As

LapD, LAP, Leucine aminopeptidase P

Function

Gene Ontology (GO) Annotations (2 terms)

Molecular Function (1 term)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

enables aminopeptidase activity

inferred from direct assay

(Hall, 1986)

Terms Based on Predictions or Assertions (0 terms)

Biological Process (0 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (0 terms)

Cellular Component (1 term)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

located_in cytosol

inferred from direct assay

(Hall, 1986)

Terms Based on Predictions or Assertions (0 terms)

Gene Group / Complex (FlyBase)

Protein Family (UniProt)

Protein Signatures (InterPro)

Summaries

Phenotypic Description (Red Book; Lindsley and Zimm 1992)

Lap-D: Leucine aminopeptidase D

Structural gene for leucine aminopeptidase D[LAP-D(E.C.3.4.1.1)], one of six such activities revealed by starch-gel electrophoresis and a staining with L-leucil-B-napthylamide and Fast Black K salt; enzyme monomeric; molecular weight = 280,000 daltons; pH optimum 7.6; may be Zn metalloenzyme. Found in larvae and pupae, but not adults; highest in young larvae. Localized to larval midgut where levels are induced by dietary substrate.

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Transcript Data

Annotated Transcripts

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Polypeptides with Identical Sequences

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Lap-D using the Feature Mapper tool.

External Data

Crossreferences

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

NA

Transcript Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

Additional Descriptive Data

The LapP protein in earlyembryos is present in an insoluble form and is thought to be membrane orvesicle associated.

Activity is highest in larvae and adults. It is found mainly in the gut but is also found in the Malpighian tubules.

LapG activity is lowin early embryos and rises from 15 hours through hatching. A rise inLapG activity after eclosion appears to be dependent upon ingestion ofdietary protein.

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in cytosol

inferred from direct assay

(Hall, 1986)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Lap-D in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Lap-D

View exonic expression by tissue for Dmel\Lap-D

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 2 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 0 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Lap-D

Transgenic constructs containing regulatory region of Lap-D

Aberrations (Deficiencies and Duplications) ( 0 )

Inferred from experimentation ( 0 )

Inferred from location ( 0 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Other Phenotypes

Allele

electrophoretic variant

Phenotype manifest in

Allele

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (0)

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (0)

Mus musculus (laboratory mouse) (0)

Xenopus tropicalis (Western clawed frog) (0)

Danio rerio (Zebrafish) (0)

Caenorhabditis elegans (Nematode, roundworm) (0)

Anopheles gambiae (African malaria mosquito) (0)

Arabidopsis thaliana (thale-cress) (0)

Saccharomyces cerevisiae (Brewer's yeast) (0)

Schizosaccharomyces pombe (Fission yeast) (0)

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:Lap-D. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (0)

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

Summary of Genetic Interactions

Interaction Browsers

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Linkouts

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

Class of Gene

gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

Cytogenetic map

Sequence location

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

(Choudhary et al., 1992)

3-98.3

(Falke and MacIntyre, 1966)

Left of (cM)

Right of (cM)

Notes

No recombinants with Lap-A.

Stocks and Reagents

Stocks (2)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

4016

Est-6^S α-Est5^F Fdh^S Lap-D^F Acph-1^A Tpi⁶/TM6, Est-6^F α-Est5^S Fdh^F Lap-D^S Acph-1^B Tpi⁴

Kyoto

107556

Est-6^S α-Est5^F Fdh^S Lap-D^F Acph-1^A Tpi⁶/TM6, Est-6^F α-Est5^S Fdh^F Lap-D^S Acph-1^B Tpi⁴

Genomic Clones (0)

cDNA Clones (0)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

A screen to identify modifiers for the Leucine aminopeptidase locates them on the X, second and third chromosomes.

(Hiraizumi et al., 1993)

Lap-A and Lap-D gene products may generate tetramers and dimers of polypeptide subunits. The gut form (LapG) may be a tetramer, in 5 forms due to different combinations of Lap-A and Lap-D subunits. The smaller LapP (pupal hemolymph form) proposed to be a dimer in three forms. Monomers may account for the more highly mobile activities.

(Seymour-Jones and Collett, 1992)

No difference in allele fixed in lines selected over 700 generations for high (negative) and low (positive) geotaxis.

(Stoltenberg et al., 1992)

The developmental variation and tissue distributions of LapP and LapG have been determined.

(Hall, 1988)

LapP and LapG expression is regulated during development: they represent part of a coordinated system for mobilizing stored protein from yolk.

(Hall, 1988)

Lap-D consists of two isozymes, designated LapP and LapG. In pupae most LapP activity results from LapP (pupal) and in larvae and adults from LapG (gut). LapP and LapG may be separated by electrophoresis, large differences in net electrical charge exist between LapP and LapG.

(Hall, 1986)

Structural gene for leucine aminopeptidase D (LAP-D), one of six such activities revealed by starch-gel electrophoresis and a staining with L-leucil-B-napthylamide and Fast Black K salt; enzyme monomeric; molecular weight = 280,000 daltons; pH optimum 7.6; may be Zn metalloenzyme. Found in larvae and pupae, but not adults; highest in young larvae. Localized to larval midgut where levels are induced by dietary substrate.

Relationship to Other Genes

Source for database merge of

Additional comments

Characterization of LAP genes "Lap-A" and "Lap-D" from the distribution and enzymatic activity of their proteins reveals a striking resemblance to the description of Jonah genes in 99C ("Jon99Co", "Jon99Ci", "Jon99Cii" and "Jon99Ciii") and 99F ("Jon99Fi" and "Jon99Fii") and the pattern of expression of their transcripts. At least one of the Jonah genes in 99C or 99F may correspond to "Lap-A" or "Lap-D".

(Collett and Seymour-Jones, 1992)

A third, unnamed and uncharacterized allele is alluded to by Sakai et al. (1968).

Nomenclature History

Source for database identify of

Nomenclature comments

Etymology

Synonyms and Secondary IDs (9)

Reported As

Symbol Synonym

LAP

(Bondinas et al., 2002, Bondinas et al., 2001)

LAP-D

(Choudhary et al., 1992)

Lap

(Matthews and Munstermann, 1994)

Lap-D

LapD

(Adrion et al., 2015, Hall, 1986)

Name Synonyms

Leucine aminopeptidase D

Leucine aminopeptidase P

(Hall, 1988, Hall, 1988)

leucine aminopeptidase

(Bondinas et al., 2001)

leucyl aminopeptidase

(Cornwell and Beckingham, 2002)

Secondary FlyBase IDs

FBgn0013289
FBgn0013290

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 0 )

References (24)

NA

Report Sections