FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
aPKC, l(2)gl
FlyBase ID
FBig0000000103
Interaction Network
Interactions Browser links
aPKC network
l(2)gl network
Reported Interactions
FBrf0157155-13.coIP.WB
Reference
Betschinger et al., 2003
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
l(2)gl 

Lgl

bait
aPKC 

aPKC

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Phosphorylated isoforms of l(2)gl are absent when aPKC is knocked down by RNAi.

(Betschinger et al., 2003)

Source was cell extract of S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

(Betschinger et al., 2003)
FBrf0157155-3.coIP.WB
Reference
Betschinger et al., 2003
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage 6embryonic stage 11embryonic stage 9early extended germ band stageembryonic stage 8embryonic stage 10embryonic stage 7
Cell line used
Participants
Corresponds to
Reported as
Role
Note
l(2)gl 

Lgl

bait
aPKC 

aPKC

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Betschinger et al., 2003)
FBrf0203052-2.coIP.WB
Reference
Tian and Deng, 2008
Description
physical association
Assay
Collection
Source/Stage
ovary / femaleadult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
l(2)gl 

Lgl

bait
aPKC 

aPKC

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was ovaries of transgenic fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Tian and Deng, 2008)
FBrf0206088-8.coIP.WB
Reference
Wirtz-Peitz et al., 2008
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
l(2)gl 

Lgl

bait
aPKC 

aPKC

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

baz and l(2)gl assemble into distinct complexes with aPKC. No interaction of baz with l(2)gl was observed.

(Wirtz-Peitz et al., 2008)

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Wirtz-Peitz et al., 2008)
FBrf0218395-623.DPiM
Reference
Guruharsha et al., 2011
Description
physical association
Assay
Collection
DPiM-2
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
l(2)gl 
unspecified role
l(2)gl 
unspecified role
l(2)gl 
unspecified role
aPKC 
unspecified role
aPKC 
unspecified role
aPKC 
unspecified role
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

HGScore = 137.18883

FBrf0222476-4.coIP.WB
Reference
Goh et al., 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
ML-DmBG3-c2
Participants
Corresponds to
Reported as
Role
Note
aPKC 

aPKC

bait
l(2)gl 

Lgl

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of BG3 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

(Goh et al., 2013)
FBrf0233695-1.coIP.MS
Reference
Calero-Cuenca et al., 2016
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
aPKC 

aPKC

bait

Tetra tag

l(2)gl 

Lgl

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Calero-Cuenca et al., 2016)
FBrf0258800-11.PLA
Reference
Portela et al., 2024
Description
physical association
Assay
Collection
Source/Stage
salivary gland / third instar larval stageeye disc / third instar larval stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
l(2)gl 

Lgl

neutral component

GFP

l(2)gl 

Lgl

neutral component

GFP

l(2)gl 

Lgl

neutral component

GFP

aPKC 

aPKC

neutral component
aPKC 

aPKC

neutral component
aPKC 

aPKC

neutral component
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was eye discs and larval salivary glands of transgenic fly line; aPKC produced from endogenous gene; l(2)gl produced from transgenic construct.

(Portela et al., 2024)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (7)