mago, tsu Physical Interaction report

General Information

Interaction Type

physical association

Interacting Genes

mago, tsu

FlyBase ID

FBig0000003783

Interaction Network

Interactions Browser links

mago network
tsu network

Reported Interactions

FBrf0157191-1.XC

Reference

Shi and Xu, 2003

Description

physical association

Assay

x-ray crystallography

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

mago

Mago

neutral component

mago

Mago

neutral component

mago

Mago

neutral component

tsu

Y14

neutral component

tsu

Y14

neutral component

tsu

Y14

neutral component

tsu

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

tsu

central region

sufficient binding region

aa 47-155

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Interaction in vitro; proteins produced as a recombinant fusion proteins in bacterial system.

(Shi and Xu, 2003)

Complex was treated with endoproteinase C before crystallization, trimming tsu (Y14) while leaving mago intact.

(Shi and Xu, 2003)

FBrf0160529-1.PD

Reference

Fribourg et al., 2003

Description

physical association

Assay

pull down

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

mago

Mago

bait

His tag

tsu

Y14

prey

tsu

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

tsu

N-terminal region

sufficient binding region

aa 1-154

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion proteins in bacterial system; prey produced as a recombinant fusion proteins in bacterial system.

(Fribourg et al., 2003)

mago and tsu were co-expressed on a bicistronic construct.

(Fribourg et al., 2003)

FBrf0160529-2.CH

Reference

Fribourg et al., 2003

Description

physical association

Assay

molecular sieving

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

mago

Mago

neutral component

mago

Mago

neutral component

mago

Mago

neutral component

tsu

Y14

neutral component

tsu

Y14

neutral component

tsu

Y14

neutral component

tsu

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

tsu

N-terminal region

sufficient binding region

aa 1-154

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Interaction in vitro; proteins produced as a recombinant fusion proteins in bacterial system.

(Fribourg et al., 2003)

Recombinant mago and tsu co-purify as a heterodimer by size-exclusion chromatography.

(Fribourg et al., 2003)

FBrf0160529-3.XC

Reference

Fribourg et al., 2003

Description

physical association

Assay

x-ray crystallography

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

mago

Mago

neutral component

mago

Mago

neutral component

mago

Mago

neutral component

tsu

Y14

neutral component

tsu

Y14

neutral component

tsu

Y14

neutral component

tsu

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

tsu

N-terminal region

sufficient binding region

aa 1-154

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Interaction in vitro; proteins produced as a recombinant fusion proteins in bacterial system.

(Fribourg et al., 2003)

mago and tsu were co-expressed on a bicistronic construct.

(Fribourg et al., 2003)

FBrf0174607-5.XC

Reference

Bono et al., 2004

Description

physical association

Assay

x-ray crystallography

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

tsu

Y14

neutral component

tsu

Y14

neutral component

tsu

Y14

neutral component

tsu

mago

Mago

neutral component

mago

Mago

neutral component

mago

Mago

neutral component

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

tsu

RNA-binding-like domain

sufficient binding region

aa 67-154

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Interaction in vitro; proteins produced as a recombinant fusion proteins in bacterial system.

(Bono et al., 2004)

Pym (residues 1-58) binds at a surface formed by both mago and the tsu RBD domain (residues 67-154) to form a ternary complex.

(Bono et al., 2004)

FBrf0209868-3.XC

Reference

Bono et al., 2010

Description

physical association

Assay

x-ray crystallography

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

mago

Mago

neutral component

mago

Mago

neutral component

mago

Mago

neutral component

tsu

Y14

neutral component

tsu

Y14

neutral component

tsu

Y14

neutral component

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Interaction in vitro; proteins produced as a recombinant fusion proteins in bacterial system.

(Bono et al., 2010)

The crystal structure of a ternary complex containing cdm, tsu and mago was captured as 3 pairwise interactions.

(Bono et al., 2010)

FBrf0209868-7.PD.MW

Reference

Bono et al., 2010

Description

physical association

Assay

pull down

molecular weight estimation by staining

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

mago

Mago

bait

GST tag

tsu

Y14

prey

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

(Bono et al., 2010)

FBrf0210119-2.coIP.WB

Reference

Lewandowski et al., 2010

Description

physical association

Assay

anti tag coimmunoprecipitation

anti tag western blot

Collection

Source/Stage

ovary / adult stage female

Cell line used

Participants

Corresponds to

Reported as

Role

Note

mago

Mago

bait

GFP tag

tsu

Tsu

prey

FLAG tag

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was ovary extract from transgenic line carrying tagged tsu and GAL4/UAS-mago constructs.

(Lewandowski et al., 2010)

FBrf0218395-1308.DPiM

Reference

Guruharsha et al., 2011

Description

physical association

Assay

experimental knowledge based

Collection

DPiM-2

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

mago

unspecified role

mago

unspecified role

mago

unspecified role

tsu

unspecified role

tsu

unspecified role

tsu

unspecified role

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

HGScore = 292.45315

FBrf0225497-8.coIP.WB

Reference

Ghosh et al., 2014

Description

physical association

Assay

anti tag coimmunoprecipitation

western blot

Collection

Source/Stage

ovary / adult stage female

Cell line used

Participants

Corresponds to

Reported as

Role

Note

mago

Mago

bait

GFP tag

tsu

Y14

prey

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was adult female ovaries of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Ghosh et al., 2014)

FBrf0243074-1.coIP.WB

Reference

Obrdlik et al., 2019

Description

physical association

Assay

anti tag coimmunoprecipitation

western blot

Collection

Source/Stage

organism / adult stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

mago

Mago

bait

GFP tag

tsu

Y14

prey

Myc tag

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was adults of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene or tagged transgenic construct.

(Obrdlik et al., 2019)

FBrf0243074-4.coIP.WB

Reference

Obrdlik et al., 2019

Description

physical association

Assay

anti tag coimmunoprecipitation

western blot

Collection

Source/Stage

organism / adult stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

tsu

Y14

bait

Myc tag

mago

Mago

prey

GFP tag

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was adults of transgenic fly line; bait produced from tagged transgenic construct; prey produced from tagged transgenic construct.

(Obrdlik et al., 2019)

External Crossreferences and Linkouts ( 1 )

Linkouts

MIST - An integrated Molecular Interaction Database

mago,tsu

References (8)

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