FlyBase Gene Report: Dmel\tsu

General Information

Symbol

Dmel\tsu

Species

D. melanogaster

Name

tsunagi

Annotation Symbol

CG8781

Feature Type

protein_coding_gene

FlyBase ID

FBgn0033378

Gene Model Status

Stock Availability

9 publicly available

Gene Summary

Core component of the splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junctions on mRNAs (PubMed:14973490, PubMed:24967911). Involved in exon definition of genes containing long introns, including the rolled/MAPK gene (PubMed:20946982, PubMed:20946983). The mago-tsu heterodimer interacts with the EJC key regulator Pym leading to EJC disassembly in the cytoplasm (PubMed:24967911). Has a role in oskar mRNA localization to the posterior pole of the developing oocyte (PubMed:11691839). (UniProt, Q9V535)

Contribute a Gene Snapshot for this gene.

All Summaries

Alliance
Auto summary
Gene Group
UniProtKB
Interactive Fly

Also Known As

Y14, Y14/Tsunagi

Key Links

Genomic Location

Cytogenetic map

Sequence location

2R:9,110,799..9,111,578 [+]

Recombination map

2-60

RefSeq locus

NT_033778 REGION:9110799..9111578

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse GBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

GO Summary Ribbons

Gene Ontology (GO) Annotations (17 terms)

Molecular Function (2 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

enables protein binding

inferred from physical interaction with UniProtKB:P82804

(assigned by UniProt )

(Bono et al., 2004, Forler et al., 2003)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

enables mRNA binding

inferred from biological aspect of ancestor with PANTHER:PTN000566947

(assigned by GO_Central )

(Gaudet et al., 2011)

inferred from sequence or structural similarity with HGNC:9905

(Mohr et al., 2001)

inferred from sequence or structural similarity

(Lasko, 2000)

Biological Process (11 terms)

Terms Based on Experimental Evidence (8 terms)

CV Term

Evidence

References

involved_in germarium-derived oocyte fate determination

inferred from mutant phenotype

(Parma et al., 2007)

involved_in microtubule cytoskeleton organization

inferred from mutant phenotype

(Mohr et al., 2001)

involved_in negative regulation of transposition, DNA-mediated

inferred from mutant phenotype

(Hayashi et al., 2014)

involved_in oocyte anterior/posterior axis specification

inferred from mutant phenotype

(Mohr et al., 2001)

involved_in oocyte dorsal/ventral axis specification

inferred from mutant phenotype

(Mohr et al., 2001)

involved_in oocyte nucleus localization involved in oocyte dorsal/ventral axis specification

inferred from mutant phenotype

(Mohr et al., 2001)

involved_in regulation of pole plasm oskar mRNA localization

inferred from mutant phenotype

(Mohr et al., 2001)

inferred from physical interaction with FLYBASE:mago; FB:FBgn0002736

(Mohr et al., 2001)

involved_in RNA splicing

inferred from mutant phenotype

(Hayashi et al., 2014)

Terms Based on Predictions or Assertions (4 terms)

CV Term

Evidence

References

involved_in establishment of pole plasm mRNA localization

traceable author statement

(Farina and Singer, 2002)

involved_in mRNA splicing, via spliceosome

inferred by curator from GO:0071013

(Herold et al., 2009)

involved_in pole plasm oskar mRNA localization

traceable author statement

(Farina and Singer, 2002, Tekotte and Davis, 2002)

involved_in RNA splicing

inferred from biological aspect of ancestor with PANTHER:PTN000566947

(assigned by GO_Central )

(Gaudet et al., 2011)

Cellular Component (4 terms)

Terms Based on Experimental Evidence (4 terms)

CV Term

Evidence

References

part_of catalytic step 2 spliceosome

inferred from high throughput direct assay

(Herold et al., 2009)

located_in cytoplasm

inferred from direct assay

(Parma et al., 2007, Mohr et al., 2001)

part_of exon-exon junction complex

inferred from physical interaction with FLYBASE:mago; FB:FBgn0002736

(Lewandowski et al., 2010)

located_in nucleus

inferred from direct assay

(Parma et al., 2007, Le Hir et al., 2001, Mohr et al., 2001)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

part_of exon-exon junction complex

inferred from biological aspect of ancestor with PANTHER:PTN000566947

(assigned by GO_Central )

(Gaudet et al., 2011)

Gene Group (FlyBase)

SPLICEOSOME COMPLEX C

SPLICEOSOME COMPLEX P

Protein Family (UniProt)

Belongs to the RBM8A family. (Q9V535)

Protein Signatures (InterPro)

Summaries

Gene Group (FlyBase)

SPLICEOSOME COMPLEX C -: Nuclear pre-mRNA splicing is catalyzed by the spliceosome which assembles in a step-wise manner. Complex B undergoes a number of structural rearrangements and U4 and U1 snRNPs dissociate to generate complex C, the catalytic spliceosome. (Adapted from PMID:24452469 and PMID:23118483).
SPLICEOSOME COMPLEX P -: Nuclear pre-mRNA splicing is catalyzed by the spliceosome which assembles in a step-wise manner. Complex P, the post-spliceosomal complex, is formed after the catalytic removal of the intron by complex C. Complex P contains the lariat intron and spliced exons. (Adapted from PMID:24452469 and PMID:23118483).

Protein Function (UniProtKB)

Core component of the splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junctions on mRNAs (PubMed:14973490, PubMed:24967911). Involved in exon definition of genes containing long introns, including the rolled/MAPK gene (PubMed:20946982, PubMed:20946983). The mago-tsu heterodimer interacts with the EJC key regulator Pym leading to EJC disassembly in the cytoplasm (PubMed:24967911). Has a role in oskar mRNA localization to the posterior pole of the developing oocyte (PubMed:11691839).

(UniProt, Q9V535)

Gene Model and Products

Number of Transcripts

1

Number of Unique Polypeptides

1

Please see the JBrowse view of Dmel\tsu for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Comments on Gene Model

Gene model reviewed during 5.44

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.49

Sequence Ontology: Class of Gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

FBtr0088567

691

165

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank

FBpp0087648

19.0

165

4.92

Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Subunit Structure (UniProtKB)

Heterodimer (via RRM domain) with mago (PubMed:11691839, PubMed:12730685, PubMed:12704080). Part of the mRNA splicing-dependent exon junction complex (EJC) complex; the core complex contains btz/CASC3, eIF4AIII, mago and tsu/RBM8A (PubMed:14973490). Interacts with Pym (via N-terminus); the interaction is direct (PubMed:24967911, PubMed:14968132). Interacts with eIF4AIII (PubMed:14973490).

(UniProt, Q9V535)

Domain

The RNA recognition motif (RRM) is involved in the interaction with mago. The RNA-binding activity of such domains is therefore uncertain.

(UniProt, Q9V535)

Crossreferences

InterPro - A database of protein families, domains and functional sites

PDB - An information portal to biological macromolecular structures

1HL6

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

Q9V535

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\tsu using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

tsu_1

Linkouts

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 4 -- 6

(Fisher et al., 2012)

embryonic stage 7 -- 8

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 9 -- 10

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 11 -- 12

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 13 -- 16

embryonic brain

(Fisher et al., 2012)

larval ventral nerve cord

(Fisher et al., 2012)

northern blot

Stage

Tissue/Position (including subcellular localization)

Reference

Comment: expression assayed in cultured cells

(Gatfield and Izaurralde, 2002)

Additional Descriptive Data

tsu transcripts are expressed in S2 cells.

(Gatfield and Izaurralde, 2002)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

oogenesis & adult stage | female

(Parma et al., 2007)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

part_of catalytic step 2 spliceosome

inferred from high throughput direct assay

(Herold et al., 2009)

located_in cytoplasm

inferred from direct assay

(Parma et al., 2007, Mohr et al., 2001)

part_of exon-exon junction complex

inferred from physical interaction with FLYBASE:mago; FB:FBgn0002736

(Lewandowski et al., 2010)

located_in nucleus

inferred from direct assay

(Parma et al., 2007, Le Hir et al., 2001, Mohr et al., 2001)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\tsu in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\tsu

View exonic expression by tissue for Dmel\tsu

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0033378

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0033378

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG8781-RA

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG8781

Flygut - An atlas of the Drosophila adult midgut

FBgn0033378

Images

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 10 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 13 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of tsu

Transgenic constructs containing regulatory region of tsu

Aberrations (Deficiencies and Duplications) ( 9 )

Inferred from experimentation ( 9 )

Gene disrupted in

(Kahsai and Cook, 2018, Kahsai et al., 2016, Christensen and Cook, 2007.3.22)

(Mohr and Boswell, 2002, Hachet and Ephrussi, 2001, Mohr et al., 2001)

(Hachet and Ephrussi, 2001)

(Mohr and Boswell, 2002, Hachet and Ephrussi, 2001, Mohr et al., 2001)

(Mohr and Boswell, 2002)

Gene not disrupted in

(Mohr and Boswell, 2002)

(Mohr and Boswell, 2002)

(Mohr and Boswell, 2002)

(Mohr and Boswell, 2002)

Inferred from location ( 0 )

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

tsu^GD14025

lethal, with Scer\GAL4^nub-AC-62

tsu^HMC04055

lethal (with Df(2R)BSC271)

tsu^EP567

lethal (with Df(2R)Np5)

lethal | female limited, with Scer\GAL4^dome-PG14

tsu^HMC04055

lethal | recessive

lethal | recessive, with Scer\GAL4^- :

tsu^EP567

lethal - all die before end of pupal stage, with Scer\GAL4^pnr-MD237

tsu^GD14025

lethal - all die during embryonic stage | germline clone | recessive, with Scer\GAL4^- :

tsu^EP567

lethal - all die during P-stage, with Scer\GAL4^Mef2.PR

tsu^GD14025

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

tsu^GD14025

viable, with Scer\GAL4^tin.CΔ4

tsu^GD14025

viable (with tsu⁵)

viable (with tsu⁶)

viable (with tsu⁷)

Sterility

Allele

female sterile, with Scer\GAL4^VP16.otu

female sterile (with tsu⁵)

female sterile (with tsu⁶)

female sterile (with tsu⁷)

female sterile | recessive

Other Phenotypes

Allele

short lived | heat sensitive, with Scer\GAL4^tin.CΔ4

tsu^GD14025

some die during embryonic stage | recessive, with Scer\GAL4^- :

tsu^EP567

some die during pupal stage, with Scer\GAL4^pnr-MD237

tsu^GD14025

Phenotype manifest in

Allele

dorsal appendage

tsu⁷

dorsal appendage (with tsu⁶)

tsu⁷

dorsal appendage (with tsu⁷)

tsu⁶

tsu⁷

egg (with tsu⁶)

tsu⁷

egg (with tsu⁷)

tsu⁶

egg | germline clone, with Scer\GAL4^- :

tsu^EP567

egg chamber (with tsu⁶)

tsu⁷

egg chamber (with tsu⁷)

tsu⁶

egg chamber | germline clone

tsu^Δ3-5

embryonic/larval brain | larval stage

tsu^GD14025

germarium region 2b | germline clone

tsu^Δ3-5

germarium region 3 | germline clone

tsu^Δ3-5

tsu⁷

ovary, with Scer\GAL4^nos.PG

tsu^{dsRNA.shRNA.UAS}

ovary, with Scer\GAL4^NP1624

tsu^{dsRNA.shRNA.UAS}

synaptonemal complex | germline clone

tsu^Δ3-5

Orthologs

Downloads

Download All DIOPT Orthologs

Download All OrthoDB Orthologs

Human Orthologs (via DIOPT v8.0)

Homo sapiens (Human) (2)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

13 of 15

Yes

Yes

1 of 15

No

Yes

Model Organism Orthologs (via DIOPT v8.0)

Mus musculus (laboratory mouse) (2)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

11 of 15

Yes

Yes

8 of 15

No

Yes

Rattus norvegicus (Norway rat) (1)

12 of 13

Yes

Yes

Xenopus tropicalis (Western clawed frog) (1)

7 of 12

Yes

Yes

Danio rerio (Zebrafish) (1)

14 of 15

Yes

Yes

Caenorhabditis elegans (Nematode, roundworm) (1)

11 of 15

Yes

Yes

Arabidopsis thaliana (thale-cress) (1)

7 of 9

Yes

Yes

Saccharomyces cerevisiae (Brewer's yeast) (0)

No records found.

Schizosaccharomyces pombe (Fission yeast) (1)

Spom\SPAC23A1.09

9 of 12

Yes

Yes

Ortholog(s) in Drosophila Species (via OrthoDB v9.1) ( EOG09190GYJ )

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila suzukii

Spotted wing Drosophila

Dsuz\DS10_00003312

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Drosophila persimilis

Drosophila willistoni

Drosophila virilis

Drosophila mojavensis

Drosophila grimshawi

Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG09150D5Y )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Musca domestica

House fly

Glossina morsitans

Tsetse fly

Gmor\GMOY006434

Lucilia cuprina

Australian sheep blowfly

Lcup\FF38_10799

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL006748

Anopheles darlingi

American malaria mosquito

Adar\ADAC005688

Anopheles gambiae

Malaria mosquito

Agam\AGAP006365

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ007339

Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W0GVL )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Danaus plexippus

Monarch butterfly

Dple\DPOGS204008-PA

Heliconius melpomene

Postman butterfly

Hmel\HMEL011247

Apis florea

Little honeybee

Aflo\2419220500

Apis mellifera

Western honey bee

Bombus impatiens

Common eastern bumble bee

Bombus terrestris

Buff-tailed bumblebee

Linepithema humile

Argentine ant

Megachile rotundata

Alfalfa leafcutting bee

Mrot\MROTU:000cfa

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG012726

Dendroctonus ponderosae

Mountain pine beetle

Tribolium castaneum

Red flour beetle

Pediculus humanus

Human body louse

Phum\PHUM390470

Rhodnius prolixus

Kissing bug

Rpro\RPRC006902

Cimex lectularius

Bed bug

Clec\CLEC008085

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI007376

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI007226

Zootermopsis nevadensis

Nevada dampwood termite

Znev\Znev_15970

Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X0GWU )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strigamia maritima

European centipede

Smar\SMAR015235

Strigamia maritima

European centipede

Smar\SMAR008546

Strigamia maritima

European centipede

Smar\SMAR009070

Strigamia maritima

European centipede

Smar\SMAR012088

Ixodes scapularis

Black-legged tick

Isca\ISCW016642

Stegodyphus mimosarum

African social velvet spider

Smim\KFM78351.1

Tetranychus urticae

Two-spotted spider mite

Turt\tetur06g02870

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_347007

Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G0PLI )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strongylocentrotus purpuratus

Purple sea urchin

Ciona intestinalis

Vase tunicate

Cint\LOC100185146

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v8.0)

Drosophila melanogaster (Fruit fly) (0)

No records found.

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Model Summary Ribbon

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 1 )

Human Ortholog

Disease

Evidence

References

RBM8A; RNA binding motif protein 8A

model of thrombocytopenia-absent radius syndrome

IEA

(FlyBase, 2019-)

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

RBM8A; RNA binding motif protein 8A

13 of 15

THROMBOCYTOPENIA-ABSENT RADIUS SYNDROME; TAR

thrombocytopenia-absent radius syndrome

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

View in Interactions Browser

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

pull down, molecular weight estimation by staining, anti bait coimmunoprecipitation, western blot, anti tag coimmunoprecipitation

(Obrdlik et al., 2019, Ghosh et al., 2014, Palacios et al., 2004)

pull down, molecular weight estimation by staining, experimental knowledge based, anti bait coimmunoprecipitation, western blot, anti tag coimmunoprecipitation, x-ray crystallography

(Ghosh et al., 2014, Guruharsha et al., 2011, Bono et al., 2004)

x-ray crystallography, pull down, molecular weight estimation by staining

(Bono et al., 2010)

x-ray crystallography, anti tag coimmunoprecipitation, western blot, anti tag western blot, pull down, experimental knowledge based, molecular weight estimation by staining, molecular sieving

(Obrdlik et al., 2019, Ghosh et al., 2014, Guruharsha et al., 2011, Bono et al., 2010, Lewandowski et al., 2010, Bono et al., 2004, Fribourg et al., 2003, Shi and Xu, 2003)

RNA-protein

Physical Interaction

Assay

References

anti tag coimmunoprecipitation, quantitative reverse transcription pcr

(Saulière et al., 2010)

anti tag coimmunoprecipitation, autoradiography

(Ghosh et al., 2012)

anti tag coimmunoprecipitation, autoradiography

(Ghosh et al., 2012)

anti tag coimmunoprecipitation, quantitative reverse transcription pcr

(Saulière et al., 2010)

anti tag coimmunoprecipitation, quantitative reverse transcription pcr

(Saulière et al., 2010)

Summary of Genetic Interactions

esyN Network Diagram

esyN Network Key:

Suppression

Enhancement

View in Interactions Browser

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

enhanceable

(Tuxworth et al., 2009)

enhanceable

(Vicente-Crespo et al., 2008)

suppressible

(Ashton-Beaucage et al., 2010)

External Data

Subunit Structure (UniProtKB)

Heterodimer (via RRM domain) with mago (PubMed:11691839, PubMed:12730685, PubMed:12704080). Part of the mRNA splicing-dependent exon junction complex (EJC) complex; the core complex contains btz/CASC3, eIF4AIII, mago and tsu/RBM8A (PubMed:14973490). Interacts with Pym (via N-terminus); the interaction is direct (PubMed:24967911, PubMed:14968132). Interacts with eIF4AIII (PubMed:14973490).

(UniProt, Q9V535 )

Linkouts

BioGRID - A database of protein and genetic interactions.

61762

DroID - A comprehensive database of gene and protein interactions.

FBgn0033378

InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.

35924

MIST (protein-protein) - An integrated Molecular Interaction Database

tsu

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

External Data

Linkouts

KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Genomic Location and Detailed Mapping Data

Chromosome (arm)

2R

Recombination map

2-60

Cytogenetic map

Sequence location

2R:9,110,799..9,111,578 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

45A7-45A7

Limits computationally determined from genome sequence between P{lacW}l(2)45Ai^k00116&P{PZ}Su(var)2-10⁰³⁶⁹⁷ and P{lacW}CG8788^k04512&P{lacW}l(2)k13412^k13412

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

45A-45A

(Mohr, 1999.7.30)

45A4-45A4

(determined by in situ hybridisation)

(Mohr et al., 2001)

Experimentally Determined Recombination Data

Location

2-60

(FlyBase, 2016)

2-58.1

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (9)

28955

y¹ v¹; P{TRiP.HM05166}attP2

14097

y¹; P{SUPor-P}tsu^KG04415/CyO; ry⁵⁰⁶

6597

w¹¹¹⁸; tsu^EP567 bw¹ speck¹/SM5

36585

y¹ sc^* v¹ sev²¹; P{TRiP.GL00545}attP2

55367

y¹ sc^* v¹ sev²¹; P{TRiP.HMC04055}attP40

122022

w¹¹¹⁸; P{EP}tsu^EP567

v107385

P{KK101333}VIE-260B

v36025

w¹¹¹⁸; P{GD14025}v36025

v341781

P{hsFLP}1, y¹ w¹¹¹⁸; P{HD_CFD00914}attP40/CyO-GFP

Genomic Clones (13)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (125)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

RE09075

Other clones

RE01574

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0033378

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0033378

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

35924

Antibody Information

Laboratory Generated Antibodies

monoclonal

(Na et al., 2016)

polyclonal

(Choudhury et al., 2016)

Commercially Available Antibodies

Other Information

Relationship to Other Genes

Source for database identify of

Source for identity of: tsu CG8781

(FlyBase, 1996-)

Source for database merge of

Additional comments

Source for identity of tsu CG8781 was sequence comparison ( date:011219 ).

(FlyBase, 1996-)

Other Comments

tsu is required to restrict the oocyte fate to a single cell during oogenesis.

(Parma et al., 2007)

The mago/tsu physical interaction is conserved, occurs in vitro and in vivo and is not RNA mediated. mago and tsu gene products localize within nuclear speckles. mago/tsu heterodimers associate with spliced mRNAs carrying an exon-exon junction complex.

(Le Hir et al., 2001)

mago and tsu are required for cell viability.

(Le Hir et al., 2001)

Origin and Etymology

Discoverer

Etymology

Gene is named "tsunagi", which is Japanese for "connection" or "link".

(Mohr et al., 2001)

Identification

External Crossreferences and Linkouts ( 43 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

35924

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

Q9V535

Other crossreferences

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0033378

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0033378

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

tsu_1

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG8781

Flygut - An atlas of the Drosophila adult midgut

FBgn0033378

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

35924

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC005086

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

Dmel_CG8781

modMine - A data warehouse for the modENCODE project

FBgn0033378

PDB - An information portal to biological macromolecular structures

1HL6

Linkouts

BioGRID - A database of protein and genetic interactions.

61762

DroID - A comprehensive database of gene and protein interactions.

FBgn0033378

DRSC - Results frm RNAi screens

FBgn0033378

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0033378

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG8781-RA

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0033378

FlyMine - An integrated database for Drosophila genomics

FBgn0033378

InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.

35924

KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.

MIST (protein-protein) - An integrated Molecular Interaction Database

tsu

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Synonyms and Secondary IDs (11)

Reported As

Symbol Synonym

CG8781

(Na et al., 2016, Brooks et al., 2015, Hosono et al., 2015, Ni et al., 2011.7.19, Ni et al., 2010.12.1, Herold et al., 2009, Keleman et al., 2009.8.5, Perkins et al., 2009, Gandhi et al., 2007, Gatfield et al., 2003, Gatfield and Izaurralde, 2002)

TSU

(Sofola et al., 2007)

Tsu

(Lewandowski et al., 2010)

Y14/Tsunagi

(Hachet and Ephrussi, 2004, Wilkie et al., 2003)

cg8781

(Lasko, 2000)

dY14

(Wu et al., 2019)

y14

(Ghosh et al., 2014, Besse et al., 2009, Rehwinkel et al., 2004, Herold et al., 2003, Anonymous, 2001, Hachet and Ephrussi, 2001)

Name Synonyms

Tsunagi

(Lewandowski et al., 2010, Nie et al., 2009, Tuxworth et al., 2009, Okamura et al., 2008)

Y14

(Tuxworth et al., 2009)

tsunagi

(Marie et al., 2017, Jakšić and Schlötterer, 2016, Rumpf et al., 2014, Handler et al., 2013, Tuxworth et al., 2009, Garcia-Lopez et al., 2008, Vicente-Crespo et al., 2008, Parma et al., 2007, Mohr et al., 2001)

Secondary FlyBase IDs

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

References (116)