Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.
Source was adult female ovaries of wild-type or transgenic fly line; bait produced from endogenous gene; prey produced from endogenous gene.
Source was adults of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene or tagged transgenic construct.