FlyBase Gene Report: Dmel\snRNP-U1-70K

General Information

Symbol

Dmel\snRNP-U1-70K

Species

D. melanogaster

Name

small ribonucleoprotein particle U1 subunit 70K

Annotation Symbol

CG8749

Feature Type

protein_coding_gene

FlyBase ID

FBgn0016978

Gene Model Status

Current

Stock Availability

11 publicly available

Gene Summary

small ribonucleoprotein particle U1 subunit 70K (snRNP-U1-70K) encodes a protein component of the U1 snRNP. The U1 snRNP combines with other snRNPs on the pre-mRNA to assemble a spliceosome. [Date last reviewed: 2019-03-14] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Gene Group
UniProtKB
Interactive Fly

Also Known As

U1-70K, snRNP70K, U1 snRNP, snRNP27D, dU1-70K

Key Links

Genomic Location

Cytogenetic map

27C7-27D1

Sequence location

2L:6,968,711..6,971,737 [-]

Recombination map

2-24

RefSeq locus

NT_033779 REGION:6968711..6971737

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (16 terms)

Molecular Function (4 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

enables mRNA binding

inferred from direct assay

(Labourier et al., 2001)

Terms Based on Predictions or Assertions (4 terms)

CV Term

Evidence

References

enables mRNA binding

inferred from sequence or structural similarity

(Lasko, 2000)

inferred from biological aspect of ancestor with PANTHER:PTN000359236

(GO Reference Genome Project, 2011-)

enables nucleic acid binding

inferred from electronic annotation with InterPro:IPR035979

(InterPro Project Members, 2004-)

enables RNA binding

inferred from electronic annotation with InterPro:IPR000504

(InterPro Project Members, 2004-)

inferred from sequence or structural similarity with UniProtKB:P08621

(Gene Ontology Curators, 2002-)

enables U1 snRNA binding

inferred from electronic annotation with InterPro:IPR034143

(InterPro Project Members, 2004-)

inferred from sequence or structural similarity with UniProtKB:P08621

(Gene Ontology Curators, 2002-)

inferred from biological aspect of ancestor with PANTHER:PTN000359237

(GO Reference Genome Project, 2011-)

Biological Process (5 terms)

Terms Based on Experimental Evidence (3 terms)

CV Term

Evidence

References

involved_in negative regulation of mRNA splicing, via spliceosome

inferred from physical interaction with FLYBASE:Psi; FB:FBgn0014870

(Labourier et al., 2001)

involved_in oogenesis

inferred from mutant phenotype

(Klusza et al., 2013)

involved_in regulation of alternative mRNA splicing, via spliceosome

inferred from high throughput mutant phenotype

(Park et al., 2004)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

involved_in mRNA splicing, via spliceosome

inferred from sequence or structural similarity with SGD:S000001323

(Mount and Salz, 2000)

inferred by curator from GO:0071011,GO:0071013

(Herold et al., 2009)

inferred from sequence or structural similarity with UniProtKB:P08621

(Gene Ontology Curators, 2002-)

inferred from biological aspect of ancestor with PANTHER:PTN000359236

(GO Reference Genome Project, 2011-)

involved_in regulation of RNA splicing

inferred from sequence or structural similarity with UniProtKB:P08621

(Gene Ontology Curators, 2002-)

Cellular Component (7 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

part_of catalytic step 2 spliceosome

inferred from high throughput direct assay

(Herold et al., 2009)

part_of precatalytic spliceosome

inferred from high throughput direct assay

(Herold et al., 2009)

Terms Based on Predictions or Assertions (5 terms)

CV Term

Evidence

References

located_in nuclear speck

inferred from sequence or structural similarity with UniProtKB:Q62376

(Gene Ontology Curators, 2002-)

located_in nucleus

inferred by curator from GO:0000381

(Park et al., 2004)

part_of spliceosomal complex

inferred from sequence or structural similarity with UniProtKB:P08621

(Gene Ontology Curators, 2002-)

part_of U1 snRNP

inferred from sequence or structural similarity with SGD:S000001323

(Mount and Salz, 2000)

inferred from sequence or structural similarity with UniProtKB:P08621

(Gene Ontology Curators, 2002-)

inferred from biological aspect of ancestor with PANTHER:PTN000359237

(GO Reference Genome Project, 2011-)

part_of U2-type prespliceosome

inferred from biological aspect of ancestor with PANTHER:PTN000359237

(GO Reference Genome Project, 2011-)

Gene Group (FlyBase)

U1 SMALL NUCLEAR RIBONUCLEOPROTEIN PARTICLE

SPLICEOSOME COMPLEX A

SPLICEOSOME COMPLEX B

Protein Family (UniProt)

Protein Signatures (InterPro)

Summaries

Gene Snapshot

Gene Group (FlyBase)

U1 SMALL NUCLEAR RIBONUCLEOPROTEIN PARTICLE -: The U1 small nuclear ribonucleoprotein particle (U1 snRNP) contains U1 snRNA and initiates spliceosome assembly by binding to the 5' splice site in pre-mRNA. (Adapted from PMID:11206553 and PMID:21441581).
SPLICEOSOME COMPLEX A -: Nuclear pre-mRNA splicing is catalyzed by the spliceosome which assembles in a step-wise manner. Complex A, the pre-spliceosome, is composed of U1 and U2 snRNPs and proteins involved in recognition of the 5' splice site and branch point. (Adapted from PMID:24452469 and PMID:23118483).
SPLICEOSOME COMPLEX B -: Nuclear pre-mRNA splicing is catalyzed by the spliceosome which assembles in a step-wise manner. After the assembly of complex A on pre-mRNA, the U4-U6 and U5 snRNPs are recruited as a preassembled tri-snRNP to form complex B. U5 snRNP binds exons at the 5' site. (Adapted from PMID:24452469 and PMID:23118483).

Protein Function (UniProtKB)

Mediates the splicing of pre-mRNA by binding to the stem loop I region of U1-snRNA (PubMed:15611175). Required during oogenesis for nurse cell chromatin dispersal (PubMed:24244416).

(UniProt, P17133)

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\snRNP-U1-70K for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry P17133)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

PDB - An information portal to biological macromolecular structures

2BN5

Comments on Gene Model

Gene model reviewed during 5.44

Gene model reviewed during 5.49

Low-frequency RNA-Seq exon junction(s) not annotated.

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

snRNP-U1-70K-RA

FBtr0079355

NM_057857

1834

448

snRNP-U1-70K-RC

FBtr0331375

NM_001273244

2948

448

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

snRNP-U1-70K-PA

FBpp0078983

52.9

448

10.65

FBpp0303793

52.9

448

10.65

P17133

NP_001260173

AGB92709

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

448 aa isoforms: snRNP-U1-70K-PA, snRNP-U1-70K-PC

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Subunit Structure (UniProtKB)

Component of the U1 snRNP (By similarity). Interacts with Psi; essential for alternative splicing of P-element transposase. Interacts with the SMN complex.

(UniProt, P17133)

Domain

Protein interactions mediated by the Arg-rich domain are not essential for viability, but do contribute to an essential U1 snRNP function.

The RRM domain mediates interaction with U1 RNA.

(UniProt, P17133)

Crossreferences

InterPro - A database of protein families, domains and functional sites

PDB - An information portal to biological macromolecular structures

2BN5

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\snRNP-U1-70K using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

snRNP-U1-70K_1

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

0.05

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism | ubiquitous

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 4 -- 6

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 7 -- 8

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 9 -- 10

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 11 -- 12

anterior midgut primordium

(Fisher et al., 2012)

central brain primordium

(Fisher et al., 2012)

head mesoderm

(Fisher et al., 2012)

organism | ubiquitous

(Fisher et al., 2012)

posterior midgut primordium

(Fisher et al., 2012)

trunk mesoderm

(Fisher et al., 2012)

ventral nerve cord primordium

(Fisher et al., 2012)

embryonic stage 13 -- 16

dorsal pharyngeal muscle cell

(Fisher et al., 2012)

embryonic brain

(Fisher et al., 2012)

embryonic/larval midgut

(Fisher et al., 2012)

larval muscle system

(Fisher et al., 2012)

larval nervous system

(Fisher et al., 2012)

organism | ubiquitous

(Fisher et al., 2012)

larval ventral nerve cord

(Fisher et al., 2012)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

part_of catalytic step 2 spliceosome

inferred from high throughput direct assay

(Herold et al., 2009)

part_of precatalytic spliceosome

inferred from high throughput direct assay

(Herold et al., 2009)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\snRNP-U1-70K in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\snRNP-U1-70K

View exonic expression by tissue for Dmel\snRNP-U1-70K

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0016978

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

33982/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0016978

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0016978

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0016978

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG8749

Flygut - An atlas of the Drosophila adult midgut

FBgn0016978

Images

FlyExpress - Embryonic expression images (BDGP data)

Show all FlyExpress processed images for Dmel\snRNP-U1-70K

Stages(s) 1-3
Stages(s) 4-6
Stages(s) 7-8
Stages(s) 9-10
Stages(s) 11-12
Stages(s) 13-16

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 6 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 8 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of snRNP-U1-70K

Transgenic constructs containing regulatory region of snRNP-U1-70K

Aberrations (Deficiencies and Duplications) ( 4 )

Inferred from experimentation ( 4 )

Gene disrupted in

Df(2L)Exel7029

(Ryder, 2004.4.26)

Df(2L)spd^j2

(Spradling et al., 1999)

Df(2L)spd

(BDGP Project Members, 1994-1999)

Gene not disrupted in

Df(2L)J-H

(Spradling et al., 1999, Beaton, 1997.3.4, BDGP Project Members, 1994-1999)

Inferred from location ( 5 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal, with Scer\GAL4^insc-Mz1407

snRNP-U1-70K^GD13212

lethal, with Scer\GAL4^VP16.otu

snRNP-U1-70K^HMS00274

lethal (with snRNP-U1-70K²)

lethal (with snRNP-U1-70K⁰²¹⁰⁷)

snRNP-U1-70K²

lethal (with snRNP-U1-70K^ON35)

snRNP-U1-70K²

lethal | recessive

snRNP-U1-70K⁰²¹⁰⁷

lethal - all die before end of pupal stage, with Scer\GAL4^pnr-MD237

snRNP-U1-70K^GD13212

lethal - all die during embryonic stage | recessive

lethal - all die during P-stage, with Scer\GAL4^Mef2.PR

snRNP-U1-70K^GD13212

viable, with Scer\GAL4^tin.CΔ4

snRNP-U1-70K^GD13212

Sterility

Allele

male semi-sterile, with Scer\GAL4^VP16.bam

snRNP-U1-70K^HMS00274

Other Phenotypes

Allele

abnormal neuroanatomy | larval stage, with Scer\GAL4^insc-Mz1407

snRNP-U1-70K^GD13212

abnormal neurophysiology | adult stage | dominant

snRNP-U1-70K²

abnormal size | third instar larval stage, with Scer\GAL4^Cg.PA

snRNP-U1-70K^HMS00274

increased cell death, with Scer\GAL4^{VP16.mat.αTub67C}

snRNP-U1-70K^HMS00274

some die during embryonic stage | recessive

some die during pupal stage, with Scer\GAL4^pnr-MD237

snRNP-U1-70K^GD13212

Phenotype manifest in

Allele

chromatin, with Scer\GAL4^{VP16.mat.αTub67C}

snRNP-U1-70K^HMS00274

egg chamber, with Scer\GAL4^{VP16.mat.αTub67C}

snRNP-U1-70K^HMS00274

embryonic/larval fat body | third instar larval stage, with Scer\GAL4^Cg.PA

snRNP-U1-70K^HMS00274

embryonic hypodermal muscle

snRNP-U1-70K⁰²¹⁰⁷

ganglion mother cell | larval stage, with Scer\GAL4^insc-Mz1407

snRNP-U1-70K^GD13212

larval neuroblast | larval stage, with Scer\GAL4^insc-Mz1407

snRNP-U1-70K^GD13212

mitochondrion | third instar larval stage, with Scer\GAL4^Cg.PA

snRNP-U1-70K^HMS00274

nurse cell, with Scer\GAL4^{VP16.mat.αTub67C}

snRNP-U1-70K^HMS00274

retina

snRNP-U1-70K²

spermatogonium, with Scer\GAL4^VP16.bam

snRNP-U1-70K^HMS00274

spermatozoon, with Scer\GAL4^VP16.bam

snRNP-U1-70K^HMS00274

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (50)

Hsap\SNRNP70

11 of 14

Yes

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Hsap\RBMY1A1

1 of 14

Hsap\RBMY1B

1 of 14

Hsap\RBMY1C

1 of 14

Yes

Hsap\RBMY1D

1 of 14

Hsap\RBMY1E

1 of 14

Yes

Hsap\RBMY1F

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (30)

Rnor\Snrnp70

9 of 14

Yes

Rnor\Cstf2

2 of 14

Rnor\Cstf2t

2 of 14

Rnor\Snrnp35

2 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Mus musculus (laboratory mouse) (43)

Mmus\Snrnp70

10 of 14

Yes

Mmus\Cstf2

2 of 14

Mmus\Snrnp35

2 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Mmus\Gm15080

1 of 14

Yes

Mmus\Gm15085

1 of 14

Yes

Mmus\Gm15091

1 of 14

Yes

Mmus\Gm15093

1 of 14

Yes

Mmus\Gm15097

1 of 14

Yes

Mmus\Gm15107

1 of 14

Yes

Mmus\Gm15114

1 of 14

Yes

Mmus\Gm15127

1 of 14

Yes

Mmus\Gm15128

1 of 14

Yes

Mmus\Hnrnpa2b1

1 of 14

Mmus\Hnrnpab

1 of 14

Mmus\Luzp4

1 of 14

Yes

Mmus\Ott

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Xenopus tropicalis (Western clawed frog) (23)

Xtro\snrnp70

9 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Xtro\LOC100490872

1 of 13

Xtro\pabpc1l

1 of 13

Xtro\raver1

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Danio rerio (Zebrafish) (38)

Drer\snrnp70

11 of 14

Yes

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Drer\rrs1

1 of 14

Drer\si:ch73-190f9.4

1 of 14

Yes

Drer\si:dkeyp-34c12.1

1 of 14

Yes

Drer\snrnp35

1 of 14

Yes

Drer\srsf2a

1 of 14

Drer\srsf2b

1 of 14

Drer\srsf10b

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (24)

Cele\rnp-7

11 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Anopheles gambiae (African malaria mosquito) (27)

Agam\AgaP_AGAP006755

9 of 12

Yes

2 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Yes

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Arabidopsis thaliana (thale-cress) (66)

Atha\U1-70K

10 of 13

Yes

2 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Atha\AT2G21440

1 of 13

Atha\AT2G21690

1 of 13

Atha\AT2G27330

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Atha\AT3G46020

1 of 13

Yes

Atha\AT3G48840

1 of 13

Atha\AT4G13860

1 of 13

Yes

Atha\AT4G20030

1 of 13

Yes

Atha\AT4G33930

1 of 13

Yes

Atha\AT5G03580

1 of 13

Atha\AT5G06210

1 of 13

Yes

Atha\AT5G09880

1 of 13

Atha\AT5G19350

1 of 13

Atha\AT5G19960

1 of 13

Yes

Atha\AT5G41690

1 of 13

Atha\AT5G52545

1 of 13

Atha\AT5G54580

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Atha\LIF2

1 of 13

Atha\ML1

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Saccharomyces cerevisiae (Brewer's yeast) (11)

Scer\SNP1

10 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Scer\NSR1

1 of 13

Yes

Scer\PAB1

1 of 13

Scer\REA1

1 of 13

Schizosaccharomyces pombe (Fission yeast) (10)

Spom\usp101

10 of 12

Yes

Spom\crp79

1 of 12

Yes

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:snRNP-U1-70K. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (20)

2 of 13

1 of 13

1 of 13

1 of 13

1 of 13

fne

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

ssx

1 of 13

Sxl

1 of 13

tra2

1 of 13

trv

1 of 13

tsu

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 2 )

Allele

Disease

Interaction

References

snRNP-U1-70K^HMS00274

exacerbates cancer

modeled by Rbf^HMS03004

(Parkhitko et al., 2021)

exacerbates tauopathy

modeled by Hsap\MAPT^V337M.UAS

(Hsieh et al., 2019)

snRNP-U1-70K^GD13212

exacerbates tauopathy

modeled by Hsap\MAPT^V337M.UAS

(Hsieh et al., 2019)

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

SNRNP70; small nuclear ribonucleoprotein U1 subunit 70

11 of 14

180740

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

snRNP-U1-70K - ApepP

experimental knowledge based

(Guruharsha et al., 2011)

snRNP-U1-70K - Arglu1

experimental knowledge based, anti bait coimmunoprecipitation, quantitative reverse transcription pcr

(Chan and Pek, 2023, Guruharsha et al., 2011)

snRNP-U1-70K - CG10657

experimental knowledge based

(Guruharsha et al., 2011)

snRNP-U1-70K - CG3107

experimental knowledge based

(Guruharsha et al., 2011)

snRNP-U1-70K - CG4119

experimental knowledge based

(Guruharsha et al., 2011)

snRNP-U1-70K - Nagk

experimental knowledge based

(Guruharsha et al., 2011)

snRNP-U1-70K - Nkap

experimental knowledge based

(Guruharsha et al., 2011)

snRNP-U1-70K - Prp40

experimental knowledge based

(Guruharsha et al., 2011)

snRNP-U1-70K - Psi

nuclear magnetic resonance, pull down, autoradiography

(Ignjatovic et al., 2005)

snRNP-U1-70K - SmD2

experimental knowledge based

(Guruharsha et al., 2011)

snRNP-U1-70K - SmF

experimental knowledge based

(Guruharsha et al., 2011)

snRNP-U1-70K - Srrm234

experimental knowledge based

(Guruharsha et al., 2011)

snRNP-U1-70K - Ssb-c31a

experimental knowledge based

(Guruharsha et al., 2011)

snRNP-U1-70K - U2A

experimental knowledge based

(Guruharsha et al., 2011)

snRNP-U1-70K - Ubx

proximity-dependent biotin identification, Identification by mass spectrometry, anti tag coimmunoprecipitation, anti tag western blot

(Carnesecchi et al., 2020)

snRNP-U1-70K - abs

experimental knowledge based

(Guruharsha et al., 2011)

snRNP-U1-70K - eIF4E1

anti bait coimmunoprecipitation, western blot

(Graham et al., 2011)

snRNP-U1-70K - fl(2)d

anti bait coimmunoprecipitation, western blot

(Penn et al., 2008)

snRNP-U1-70K - hfp

experimental knowledge based

(Guruharsha et al., 2011)

snRNP-U1-70K - pps

anti bait coimmunoprecipitation, western blot

(Johnson et al., 2010)

snRNP-U1-70K - snRNP-U1-C

experimental knowledge based

(Guruharsha et al., 2011)

snRNP-U1-70K - snf

experimental knowledge based, anti bait coimmunoprecipitation, western blot

(Guruharsha et al., 2011, Johnson et al., 2010)

RNA-protein

Physical Interaction

Assay

References

snRNP-U1-70K - Arglu1

experimental knowledge based, anti bait coimmunoprecipitation, quantitative reverse transcription pcr

(Chan and Pek, 2023, Guruharsha et al., 2011)

Summary of Genetic Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

snRNP-U1-70K

suppressible

snRNP-U1-70K::Hsap\SRSF1

(Salz et al., 2004, Cline et al., 1999)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

snf|Sxl

enhanceable

snRNP-U1-70K

(Cline et al., 1999)

External Data

Subunit Structure (UniProtKB)

Component of the U1 snRNP (By similarity). Interacts with Psi; essential for alternative splicing of P-element transposase. Interacts with the SMN complex.

(UniProt, P17133 )

Linkouts

BioGRID - A database of protein and genetic interactions.

60131

DroID - A comprehensive database of gene and protein interactions.

FBgn0016978

MIST (genetic) - An integrated Molecular Interaction Database

33982

MIST (protein-protein) - An integrated Molecular Interaction Database

33982

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

Spliceosome

Class of Gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-24

Cytogenetic map

27C7-27D1

Sequence location

2L:6,968,711..6,971,737 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

27C7-27D1

Limits computationally determined from genome sequence between P{PZ}smt3⁰⁴⁸⁴¹&P{PZ}smt3⁰⁴⁴⁹³ and P{PZ}snRNP70K⁰²¹⁰⁷

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

27D1-27D2

(determined by in situ hybridisation)

(Spradling et al., 1999, Mancebo et al., 1990)

27D1-27D2

(BDGP Project Members, 1994-1999)

Experimentally Determined Recombination Data

Location

2-24

(FlyBase, 2016)

2-22.2

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (11)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

80445

y¹ v¹; P{TRiP.HMS06025}attP40

11177

P{PZ}snRNP-U1-70K⁰²¹⁰⁷ cn¹/CyO; ry⁵⁰⁶

33396

y¹ sc^* v¹ sev²¹; P{TRiP.HMS00274}attP2

FlyORF

F001301

M{UAS-snRNP-U1-70K.ORF}ZH-86Fb

F001170

M{UAS-snRNP-U1-70K.ORF.3xHA}ZH-86Fb

Kyoto

123664

w¹¹¹⁸; P{RS3}snRNP-U1-70K^CB-5499-3

NIG-Fly

M2L-1983

y¹ w¹¹¹⁸; snRNP-U1-70K^SK7 P{neoFRT}40A/CyO-Tb

HMS00274

y¹ sc^* v¹ sev²¹; P{TRiP.HMS00274}attP2

2LG-1588

y² VhaAC39-1^cho-1 v¹; P{U6-snRNP-U1-70K.NIG.sgRNA}attP40/CyO

VDRC

v23150

w¹¹¹⁸; P{GD13212}v23150

v23151

w¹¹¹⁸; P{GD13212}v23151

Genomic Clones (14)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (48)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0016978

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0016978

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

RNAi screen using dsRNA made from templates generated with primers directed against this gene results in a long metaphase spindle with misaligned chromosomes when assayed in S2 cells. This phenotype can be observed when the screen is performed with or without Cdc27 dsRNA.

(Goshima et al., 2007)

A sequence comparison and numerical analysis of the RRM-containing (RNA recognition motif) proteins suggests that functionally related RRM-containing proteins have significant sequence similarities in their RRMs.

(Kim and Baker, 1993)

Isolated from a genomic library using a human U1 70K snRNP cDNA clone as a probe.

(Mancebo et al., 1990)

snRNP70K has been cloned and sequenced.

(Mancebo et al., 1990)

"AQ025594; BDGP:l (2)02107" was stated as revision.

Relationship to Other Genes

Source for database merge of

Source for merge of: snRNP70K snRNP27D

(Mount, 1999.5.23)

Source for merge of: snRNP27D l(2)02107

(BDGP Project Members, 1994-1999)

Additional comments

Nomenclature History

Source for database identify of

Source for identity of: snRNP70K CG8749

(Wortman, 1999.11)

Nomenclature comments

Etymology

Synonyms and Secondary IDs (25)

Reported As

Symbol Synonym

70K

(Welin Henriksson et al., 1999, Paterson et al., 1991)

CG8749

(National Institute of Genetics Fly Stocks, 2022-, Abramczuk et al., 2017, Çiçek et al., 2016, Wu et al., 2016, Brooks et al., 2015, Horan et al., 2015, Lin et al., 2015, Lu et al., 2014, Schertel et al., 2013, Page et al., 2012, Ni et al., 2010.12.1, Herold et al., 2009, Perkins et al., 2009, Goshima et al., 2007, Jensen et al., 2006, McIntyre et al., 2006, Mount and Salz, 2000)

Dm70K

(Henriksson and Pettersson, 1997, Henriksson and Pettersson, 1996)

SnRNP-U1-70K

(Zhou et al., 2019)

(Kim et al., 2010)

U1 70K

(Mancebo et al., 1990)

U1 70K snRNP

(Tseng et al., 1998)

U1 70k

(Kroiss et al., 2008)

U1 snRNP

(Nagengast et al., 2004, Chaouki et al., 2003, Nagengast and Salz, 2003, Maniatis and Tasic, 2002, Blencowe, 2000, Smith and Valcarcel, 2000)

U1-70K

(Chan and Pek, 2023, Hsieh et al., 2019, Moschall et al., 2017, Wu et al., 2016, Lu et al., 2014, Johnson et al., 2010, Herold et al., 2009, Carr and Nagengast, 2008, Park et al., 2004, Salz et al., 2004, Nagengast et al., 2003, Nagengast et al., 2002, Boucher et al., 2001, Labourier and Rio, 2001, Cline et al., 1999, Fukami-Kobayashi et al., 1993)

U1-70k

(Graham et al., 2011, Penn et al., 2008, Ignjatovic et al., 2005)

U170K

(Penn et al., 2008, Nagengast and Salz, 2004)

U1snRNP-70K

(Voelker et al., 1991)

dU1-70K

(Allemand et al., 2002, Labourier et al., 2001)

l(2)02107

snRNP

(Yannoni and White, 1997)

snRNP 70K

(Horan et al., 2015)

snRNP-U1-70K

(Dyson et al., 2025, Palu et al., 2022, Parkhitko et al., 2021, Berson et al., 2019, Gao et al., 2019, Brewer-Jensen et al., 2016, Jakšić and Schlötterer, 2016, Brooks et al., 2015, Kwong et al., 2015, Li et al., 2015, Stoiber et al., 2015, Klusza et al., 2013, Kwon et al., 2013)

snRNP27D

(Spradling et al., 1999)

snRNP70K

(Schertel et al., 2013, Taliaferro et al., 2013, Neumüller et al., 2011, Zhou et al., 2008, Goshima et al., 2007, Lecuyer et al., 2007, Sofola et al., 2007, Jensen et al., 2006, McIntyre et al., 2006)

snRNPU1-70K

(Carnesecchi et al., 2020, Singh et al., 2019)

snRnP-U1-70K

(Choudhury et al., 2024)

Name Synonyms

U1 snRNP-specific 70K protein

(Labourier et al., 2001)

small nuclear ribonucleoprotein 70K

small ribonucleoprotein particle U1 subunit 70K

Secondary FlyBase IDs

FBgn0003458
FBgn0010508

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 54 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

33982

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

P17133

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

P17133

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

M9PC89

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

P17133

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0016978

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

33982/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0016978

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0016978

FlyMine - An integrated database for Drosophila genomics

FBgn0016978

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG8749

MARRVEL_MODEL - MARRVEL (model organism gene)

33982

PDB - An information portal to biological macromolecular structures

2BN5

Linkouts

BioGRID - A database of protein and genetic interactions.

60131

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0016978

DroID - A comprehensive database of gene and protein interactions.

FBgn0016978

DRSC - Results frm RNAi screens

FBgn0016978

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

snRNP-U1-70K_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0016978

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0016978

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG8749

Flygut - An atlas of the Drosophila adult midgut

FBgn0016978

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0016978

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC002933

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

Spliceosome

MIST (genetic) - An integrated Molecular Interaction Database

33982

MIST (protein-protein) - An integrated Molecular Interaction Database

33982

References (126)

The following external sites may use different assemblies or annotations than FlyBase.

0.05

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