FlyBase Allele Report: Dmel\snRNP-U1-70K[HMS00274]

General Information

Symbol

Dmel\snRNP-U1-70K^HMS00274

Species

D. melanogaster

Name

FlyBase ID

FBal0257173

Feature type

allele

Associated gene

Dmel\snRNP-U1-70K

Associated Insertion(s)

Carried in Construct

P{TRiP.HMS00274}

Also Known As

snRNP-U1-70K RNAi, HMS00274, UAS-snRNP-U1-70K RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Ni et al., 2010.12.1)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Ni et al., 2010.12.1)

Mutagen

in vitro construct

(Ni et al., 2010.12.1)

Progenitor genotype

Carried in construct

P{TRiP.HMS00274}

Cytology

Description

UAS regulatory sequences drive expression of a short inverted repeat.

(Ni et al., 2010.12.1)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

dsRNA-HMS00274

Also carries

loxP

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates tauopathy

modeled by Hsap\MAPT^V337M.UAS

(Hsieh et al., 2019)

exacerbates cancer

modeled by Rbf^HMS03004

(Parkhitko et al., 2021)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal size | third instar larval stage, with Scer\GAL4^Cg.PA

(Zhou et al., 2019)

increased cell death, with Scer\GAL4^{VP16.mat.αTub67C}

(Klusza et al., 2013)

lethal, with Scer\GAL4^VP16.otu

(Yan and Perrimon, 2015)

male semi-sterile, with Scer\GAL4^VP16.bam

(Wu et al., 2016)

Phenotype Manifest In

Detailed Description

Statement

Reference

Expression of snRNP-U1-70K^HMS00274 under the control of Scer\GAL4^ninaE.PU does not affect electroretinogram amplitude in 5 day old adults, compared to controls.

(Hsieh et al., 2019)

Expressing snRNP-U1-70K^HMS00274 under the control of Scer\GAL4^Cg.PA leads to fragmented and clustered mitochondria in the third instar larval fat body (as assessed by MitoGFP).

(Zhou et al., 2019)

Expression of snRNP-U1-70K^HMS00274 driven by Scer\GAL4^{bam.T:Hsim\VP16} leads to almost complete male sterility, along with defects in spermatogonial differentiation and absence of mature sperm.

(Wu et al., 2016)

Expression of snRNP-U1-70K^HMS00274 under the control of Scer\GAL4^{mat.αTub67C.T:Hsim\VP16} results in late-stage egg chambers showing multiple defects, including developmental arrest and increased apoptosis, and occasional defects in nurse cell chromatin dispersal, as compared to wild type.

(Klusza et al., 2013)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

snRNP-U1-70K^HMS00274, Scer\GAL4^GMR.PU is an enhancer of abnormal size | adult stage phenotype of Hsap\MAPT^V337M.UAS, Scer\GAL4^GMR.PU

(Hsieh et al., 2019)

snRNP-U1-70K^HMS00274, Scer\GAL4^GMR.PU is an enhancer of visible | adult stage phenotype of Hsap\MAPT^V337M.UAS, Scer\GAL4^GMR.PU