FB2026_02 , released June 18, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Cdk1, CycB
FlyBase ID
FBig0000015825
Interaction Network
Interactions Browser links
Cdk1 network
CycB network
Reported Interactions
FBrf0068557-2.coIP.WB
Reference
Knoblich et al., 1994
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage 11
Cell line used
Participants
Corresponds to
Reported as
Role
Note
CycB 

cyclin B

bait
Cdk1 

PSTAIR

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Cdk1 detected using antibody to the PSTAIR epitope.

(Knoblich et al., 1994)

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Knoblich et al., 1994)
FBrf0068557-5.coIP.WB
Reference
Knoblich et al., 1994
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage 11
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Cdk1 

cdc2

bait
CycB 

Cyclin B

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Knoblich et al., 1994)
FBrf0090786-3.Y2H
Reference
Sauer et al., 1996
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Cdk1 

cdc2

bait

fused to LexA DNA-binding domain

CycB 

cyclin B

prey

fused to B42 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast LexA-BD/B42-AD

(Sauer et al., 1996)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Sauer et al., 1996)
FBrf0091107-6.coIP.WB
Reference
Lane et al., 1996
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Cdk1 

Cdk1

bait
CycB 

CycB

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Lane et al., 1996)
FBrf0105853-2.coIP.WB
Reference
Jacobs et al., 1998
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage 1embryonic stage 4embryonic stage 3embryonic cycle 6embryonic cycle 5embryonic cycle 8embryonic cycle 7embryonic cycle 3embryonic cycle 4embryonic cycle 9embryonic stage 2
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Cdk1 

CDK1

bait
CycB 

CYCB

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Bait endogenous to embryonic extract.

(Jacobs et al., 1998)

Prey endogenous to embryonic extract.

(Jacobs et al., 1998)
FBrf0139669-6.coIP.WB
Reference
Jager et al., 2001
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Cdk1 

Cdk1

bait

Myc tag

CycB 

CYCB

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Jager et al., 2001)

Positive control.

(Jager et al., 2001)
FBrf0160729-1.coIP.WB
Reference
Leismann and Lehner, 2003
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Cdk1 

Cdk1

bait

Myc tag

CycB 

CYCB

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Leismann and Lehner, 2003)
FBrf0183813-1.coIP.WB
Reference
Stumpff et al., 2004
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
CycB 

CycB

bait
Cdk1 

Cdk1

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

CycB-Cdk1 interaction not affected in an Wee1 mutant background.

(Stumpff et al., 2004)

Source was 1-2 hr embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Stumpff et al., 2004)
FBrf0187336-1.coIP.WB
Reference
Stumpff et al., 2005
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
CycB 

CycB

bait
Cdk1 

Cdk1

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

The amount of Cdk1 recovered from the extract of transgenic embryos containing six copies of CycB is about 2-fold higher than the amount recovered from wild-type or dwee1 mutant embryo extracts.

(Stumpff et al., 2005)

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Stumpff et al., 2005)
FBrf0218395-3909.DPiM
Reference
Guruharsha et al., 2011
Description
physical association
Assay
Collection
DPiM-2
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
CycB 
unspecified role
CycB 
unspecified role
CycB 
unspecified role
Cdk1 
unspecified role
Cdk1 
unspecified role
Cdk1 
unspecified role
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

HGScore = 420.65435

FBrf0218404-48.coIP.WB
Reference
Mohd-Sarip et al., 2012
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Cdk1 

CDC2

bait
CycB 

CYC-B

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Mohd-Sarip et al., 2012)
FBrf0218404-52.coIP.WB
Reference
Mohd-Sarip et al., 2012
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
CycB 

CYC-B

bait
Cdk1 

CDC2

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Mohd-Sarip et al., 2012)
FBrf0223715-2.coIP.WB
Reference
Ayeni et al., 2014
Description
physical association
Assay
Collection
Source/Stage
wing disc / third instar larval stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Cdk1 

Cdk1

bait

Venus tag

CycB 

Cyclin B

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was larval wing discs of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Ayeni et al., 2014)

Cdk1 T14A and Y15F do not disrupt the interaction of Cdk1 with CycA or CycB.

(Ayeni et al., 2014)

Positive control.

(Ayeni et al., 2014)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (11)