FB2026_02 , released June 18, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Gem3, Smn
FlyBase ID
FBig0000016622
Interaction Network
Interactions Browser links
Gem3 network
Smn network
Reported Interactions
FBrf0206300-1.coIP.WB
Reference
Cauchi et al., 2008
Description
physical association
Assay
Collection
Source/Stage
hypodermal muscle cell of A1-7 / third instar larval stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Smn 

SMN

bait
Gem3 

Gemin3

prey

CFP tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of transgenic fly line; bait produced from endogenous gene; prey produced from tagged transgenic construct.

(Cauchi et al., 2008)
FBrf0206864-1.coIP.WB
Reference
Shpargel et al., 2009
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
Gem3 

dGem3

bait

Myc tag

Gem3 
Smn 

dSmn

prey

GFP tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Gem3 
C terminal
unspecified role
aa 572-1028
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was S2 cell line transfected with tagged Smn and Gem3 constructs.

(Shpargel et al., 2009)
FBrf0206864-2.PD.WB
Reference
Shpargel et al., 2009
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Smn 

dSmn

bait

GST pull down

Gem3 

dGem3

prey

His tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Cloned reagents; interaction in solution.

(Shpargel et al., 2009)
FBrf0206864-3.coIP.WB
Reference
Shpargel et al., 2009
Description
physical association
Assay
Collection
Source/Stage
organism / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Gem3 

dGem3

bait

Flag tag

Smn 

dSmn

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult extract from a transgenic line carrying tagged GAL4/UAS-Gem3 constructs in a mutant Gem3 background.

(Shpargel et al., 2009)
FBrf0218395-1589.DPiM
Reference
Guruharsha et al., 2011
Description
physical association
Assay
Collection
DPiM-2
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Gem3 
unspecified role
Gem3 
unspecified role
Gem3 
unspecified role
Smn 
unspecified role
Smn 
unspecified role
Smn 
unspecified role
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

HGScore = 63.42118

FBrf0226016-3.coIP.WB
Reference
Praveen et al., 2014
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
Gem3 

dGem3

bait

Myc tag

Smn 

dSMN

prey

FLAG tag

Smn 
Smn 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Smn 
YG box self-oligomerization domain residue 203
mutation disrupting interaction
aa 203

Y203C

Smn 
YG box self-oligomerization domain residue 206
mutation disrupting interaction
aa 206

G206S

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Praveen et al., 2014)
FBrf0233145-1.Y2H
Reference
Borg et al., 2016
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Smn 

SMN

bait

fused to GAL4 DNA-binding domain

Gem3 

Gem3

prey

fused to GAL4 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Borg et al., 2016)

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Borg et al., 2016)
FBrf0245892-11.coIP.MS
Reference
Maccallini et al., 2020
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Smn 

Smn

bait

GFP tag

Gem3 

Gem3

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Maccallini et al., 2020)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (6)