FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
vir, Hakai
FlyBase ID
FBig0000033629
Interaction Network
Interactions Browser links
vir network
Hakai network
Reported Interactions
FBrf0218395-3109.DPiM
Reference
Guruharsha et al., 2011
Description
physical association
Assay
Collection
DPiM-2
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
vir 
unspecified role
vir 
unspecified role
vir 
unspecified role
Hakai 
unspecified role
Hakai 
unspecified role
Hakai 
unspecified role
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

HGScore = 76.32402

FBrf0249274-16.coIP.MS
Reference
Bawankar et al., 2021
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2R+
Participants
Corresponds to
Reported as
Role
Note
Hakai 

Hakai

bait

Myc tag

vir 

Vir

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2R+ cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Bawankar et al., 2021)

The 473aa isoform of Hakai, corresponding to Hakai-PF, was used.

(Bawankar et al., 2021)
FBrf0249274-5.coIP.WB
Reference
Bawankar et al., 2021
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2R+
Participants
Corresponds to
Reported as
Role
Note
vir 

Vir

bait

Myc tag

vir 
Hakai 

Hakai

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
vir 
N-terminal domain
sufficient binding region
aa 1-130

coordinates relative to vir-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

The 473aa isoform of Hakai, corresponding to Hakai-PF, was used.

(Bawankar et al., 2021)

Source was cell extract of S2R+ cell line; bait produced from transfected construct; prey produced from transfected construct.

(Bawankar et al., 2021)
FBrf0249274-6.coIP.WB
Reference
Bawankar et al., 2021
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2R+
Participants
Corresponds to
Reported as
Role
Note
Hakai 

Hakai

bait

Myc tag

Hakai 
Hakai 
vir 

Vir

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Hakai 
N-terminal domain
sufficient binding region
aa 1-295

coordinates relative to Hakai-PF

Hakai 
RING domain
mutation disrupting interaction
aa 180,183,196,198,201,204,213,216

C180A,C183A,C196A,H198A,C201A,C204A,C213A,C216A, coordinates relative to Hakai-PF

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

The 473aa isoform of Hakai, corresponding to Hakai-PF, was used.

(Bawankar et al., 2021)

Source was cell extract of S2R+ cell line; bait produced from transfected construct; prey produced from transfected construct.

(Bawankar et al., 2021)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)